BioFlo® 310 Setup

Summary

This video demonstrates the step-by-step process of setting up the New Brunswick Bioflow 310 fermentor. Key tasks include installing the sparge line, triport liquid edition assembly, and harvest line, followed by securing the vessel’s head plate and baffle. The pH and DO sensors are calibrated and installed, and proper protective measures are taken for the autoclave cycle. Additional steps include securing filters, ensuring proper ventilation, and safeguarding equipment from heat damage. The setup concludes with installing sensors like the level sensor for specific culture needs, offering a comprehensive guide to the fermentor preparation.

Takeaways

• 😀 Install the sparge line, triport liquid edition assembly, and harvest line in the head plate before starting the setup.
• 😀 Install the baffle inside the glass vessel for proper setup.
• 😀 Secure the vessel head plate with a top metal ring and threaded screws.
• 😀 Ensure the bottom metal ring is properly secured after setting up the vessel.
• 😀 Add media to the vessel, ensuring the pH and DO sensor tips are submerged.
• 😀 Calibrate the pH sensor using two external buffer standards of known pH before starting the autoclave cycle.
• 😀 Install the pH sensor on the head plate using a compression fitting into a PG13.5 headplate port.
• 😀 Protect both pH and DO sensors with protective caps before the autoclave cycle.
• 😀 Install the sampling assembly and exhaust condenser properly.
• 😀 Secure sparge and exhaust filters with zip ties and autoclave-resistant clamps, leaving the exhaust line open for ventilation during the autoclave cycle.
• 😀 Protect the sample bottle and bearing housing from heat damage during the autoclave cycle by loosening the sample bottle and covering it with aluminum foil.

Q & A

What is the first step in setting up the New Brunswick Bioflow 310 fermentor?

The first step is to install the sparge line, the triport liquid edition assembly, and the harvest line in the head plate.

Why is it important to carefully install the baffle inside the glass vessel?

The baffle needs to be carefully installed to ensure proper mixing and to maintain the fermentation process inside the vessel.

How should the vessel head plate be secured?

The vessel head plate should be carefully installed and secured using a top metal ring with threaded screws.

What is the purpose of securing the bottom metal ring?

The bottom metal ring is secured to ensure that the glass vessel is properly sealed and stable during operation.

Where should the media be placed inside the vessel, and why?

The media should be added to the vessel at a level where the pH and DO sensors are submerged in the solution to ensure proper sensor readings.

Why is it necessary to calibrate the pH sensor before starting the autoclave cycle?

The pH sensor must be calibrated to ensure accurate pH readings during the fermentation process, which is essential for maintaining proper conditions inside the vessel.

What are compression fittings used for when installing the pH sensor?

Compression fittings are used to adjust the sensor length inside the vessel, ensuring it is properly positioned for accurate measurements.

How should the pH and DO sensors be protected during the autoclave cycle?

Both the pH and DO sensors should be covered with protective caps to prevent damage during the autoclave cycle.

What should be done to the sampling assembly before the autoclave cycle?

The sampling assembly should be installed before the autoclave cycle to ensure proper sample collection and prevent contamination.

Why should the exhaust line be left open during the autoclave cycle?

The exhaust line should be left open to allow proper ventilation, which helps prevent pressure buildup during the autoclave cycle.