Isolasi Etil-p-metoksi Sinamat - Praktikum Isolasi Senyawa Organik

Summary

This video demonstrates the step-by-step process of isolating ethyl paramethoxy cinnamate from samples using laboratory equipment. The procedure begins with sample preparation, weighing, and dissolving the material in n-hexane. It follows with a reflux process, condensation, and distillation. Afterward, the liquid undergoes decantation and further distillation for crystallization, followed by thin-layer chromatography (TLC) to test purity. The script provides a detailed guide on the tools, chemicals, and techniques involved in achieving this chemical isolation and analysis.

Takeaways

• 😀 Ensure all necessary lab equipment is prepared before starting the experiment, including glassware, thermometers, and condensors.
• 😀 Start by weighing the kencur sample (15g) before proceeding with the rest of the procedure.
• 😀 Measure and add 50ml of n-hexane to a beaker to dissolve the kencur sample.
• 😀 Stir the kencur sample in n-hexane until fully dissolved before proceeding with the reflux process.
• 😀 Set up the reflux apparatus carefully, ensuring the thermometer and condenser are properly attached and the system is leak-proof.
• 😀 Maintain a temperature range of 60-70°C during the reflux process to ensure proper extraction.
• 😀 After completing the reflux, decant the liquid to separate the extract.
• 😀 Prepare the distillation setup by installing the distillation flask, condenser, and thermometer, ensuring correct alignment for efficient distillation.
• 😀 Heat the mixture for distillation, monitoring the temperature and collecting the distillate until the process stops.
• 😀 Perform crystallization by allowing the residue from distillation to evaporate, forming yellow-brown crystals, which are the desired compounds.
• 😀 Conduct Thin Layer Chromatography (TLC) to assess the purity of the isolated compound, marking the Rf value under UV light after completing the elution process.

Q & A

What is the initial step in the experiment described in the script?

The initial step involves turning on the balance (neraca) and weighing the sample, ensuring the tare button is pressed to reset the scale before weighing approximately 15 grams of kencur (curcuma).

What is the purpose of adding n-hexane to the kencur sample?

N-hexane is added to dissolve the kencur sample, initiating the extraction process to isolate its compounds.

What is the setup for the reflux process in this experiment?

The reflux setup includes attaching a clamp to a stand, connecting a three-neck flask with a condenser, and using a thermometer to monitor the temperature while keeping it between 60-70°C during the reflux process.

Why is Vaseline applied to the condenser during setup?

Vaseline is applied to the condenser to create a better seal and ensure it stays securely attached to the three-neck flask during the reflux process.

What is the importance of maintaining a temperature range of 60-70°C during both reflux and distillation?

Maintaining a temperature of 60-70°C ensures proper extraction and distillation without overheating or causing undesired reactions, allowing for effective separation of components.

What happens to the sample after the reflux process is complete?

After the reflux process, the liquid is decanted into a beaker for filtration, where it will be further processed by distillation.

What is the next step after filtration in the distillation process?

The filtered liquid is then subjected to distillation, where the setup includes attaching a distillation flask, a condenser, and a thermometer, and heating the sample to separate volatile components.

What does the crystallization process involve in this experiment?

During crystallization, the distillation residue is transferred to a beaker, and the solvent evaporates, leaving behind crystals that are yellowish-brown in color.

What is the purpose of using Thin Layer Chromatography (TLC) in this experiment?

TLC is used to analyze the purity of the extracted sample and to identify the components by comparing the sample's Rf value under UV light.

How is the Rf value of the sample determined in the TLC process?

The Rf value is determined by measuring the distance traveled by the sample spot relative to the solvent front on the TLC plate, and then comparing it under UV light with a wavelength of 254 nm.