Penggunaan Sentrifuge untuk Hematokrit (HCT)

Summary

This video provides a step-by-step demonstration of how to use a microcentrifuge in a laboratory for blood sample testing. It covers the process of placing the blood sample and balancing it with a counterweight, securing the lid, setting the timer for 2 minutes, and starting the machine. Once the centrifugation process is complete, the operator opens the lid, retrieves the samples, and examines the result, showing the separation of plasma from the red blood cells. The tutorial offers clear instructions on handling and using the equipment safely and effectively.

Takeaways

• 😀 The procedure begins by pressing a button located beneath the centrifuge lid.
• 😀 Open the centrifuge lid and remove the protective plate from the sample holder.
• 😀 The sample holder contains numbered slots for placing samples and balancing weights.
• 😀 Place the blood sample and an equal amount of water (balance) in opposite slots to maintain equilibrium.
• 😀 Ensure the lid of the sample holder is securely closed before proceeding.
• 😀 After sealing the sample holder, close the centrifuge lid and lock it.
• 😀 Set the centrifuge timer for 2 minutes by adjusting the dial to the '0' setting and then pressing the 'on' button.
• 😀 Once the centrifuge starts, allow it to run for the preset 2 minutes.
• 😀 When the centrifuge completes the cycle, switch it off and carefully open the lid.
• 😀 Remove the blood sample and balance water, and observe the results, which show plasma separation in the sample.

Q & A

What is the function of the microcentrifuge in the laboratory?

The microcentrifuge is used for performing HCT (Hematocrit) tests, which are used to measure the proportion of blood that consists of red blood cells.

What is the first step in using the microcentrifuge?

The first step is to press the button located below the cover to turn on the machine, then open the lid.

What should be done after opening the lid of the microcentrifuge?

After opening the lid, you need to remove the cover plate, which has sample numbers on it, and place the samples in the appropriate spots.

What samples are used in the centrifuge for testing?

The samples used are blood samples and water, which acts as a balancing agent.

Why should the samples be placed in opposite directions within the centrifuge?

The samples should be placed in opposite directions to ensure the machine is balanced during operation.

What is the next step after placing the samples inside the centrifuge?

After placing the samples, you should close the cover plate tightly and then secure the lid of the centrifuge.

How is the centrifuge started after everything is set?

Once everything is set, press the 'On/Off' button, set the timer for 2 minutes, and press the 'Start' button to begin the centrifugation process.

How long should the centrifuge be operated for?

The centrifuge should be operated for 2 minutes, as indicated by the timer setting.

What should be done after the centrifuge finishes its operation?

Once the centrifuge finishes, turn it off, open the lid, and remove the samples and the balancing water.

What is the result of the centrifugation process?

The result of the centrifugation is the separation of plasma, which can be observed in the red-colored portion of the sample after the process.