Pengamatan Mitosis Pada Akar Bawang dengan Metode Squash

Summary

In this educational video, viewers are guided through the process of observing mitosis in onion root tips. The tutorial begins with cultivating the roots in a damp environment or water to encourage growth. The roots are then treated with a 0.02% colchicine solution to inhibit spindle formation and enlarge chromosomes. Afterward, a Carnoy's solution is used for cell fixation. The video covers the preparation steps, including hydrolysis and staining with aceto-orcein, to reveal the chromosomes. Finally, the observer can examine the different stages of mitosis—prophase, metaphase, anaphase, and telophase—under a microscope. The video concludes with tips for preserving the samples for future observation.

Takeaways

• 🧬 The video script discusses observing mitosis in onion root tips.
• 🌱 The first step is to grow onion roots by placing them in a moist area or submerging them in water.
• 🔪 The bottom part of the onion is cleaned to ensure quick root growth.
• 💧 The root tips are then placed in a 0.02% colchicine solution for 1-2 hours to inhibit spindle formation and enlarge chromosomes.
• 🧪 After colchicine treatment, the solution is replaced with Carnoy's solution for 2-3 hours to fix and preserve the cell condition.
• 🚫 If immediate observation is not needed, the sample is stored in 70% alcohol for preservation.
• 🔬 The purpose of the treatment is to break down cell walls to facilitate the observation of mitotic division.
• 🔥 The sample is then hydrolyzed in a reaction tube at 60°C for 15 minutes, followed by rinsing and staining with 2% aceto-orcein.
• 🎨 The staining process takes 15-30 minutes or more, depending on the sample condition.
• 🔍 After staining, excess stain is washed off with 45% acetic acid, and the slide is prepared for observation under a microscope.
• 📸 The video suggests using a 100x objective lens for observation and applying immersion oil to the cover glass for clarity.
• 🖼️ The results of the mitosis observation can be preserved using a glass slide and a cover slip for permanent and repeatable viewing.

Q & A

What is the main topic of the video script?

The main topic of the video script is learning how to observe mitosis by studying the root tips of an onion.

How should the onion root be prepared for mitosis observation?

The onion root should be prepared by placing it in a damp area or submerging it in water to encourage root growth. The bottom part of the onion should be cleaned to facilitate quick root development.

What is the purpose of using a 0.02% colchicine solution in the process?

The 0.02% colchicine solution is used to inhibit spindle fiber formation during cell division and to enlarge the chromosomes for easier observation.

What is the role of Carnoy's solution in the preparation process?

Carnoy's solution is used to fix or preserve the condition of the cells after colchicine treatment, ensuring the cells are kept in a stable state for observation.

Why is it necessary to store the sample in 70% alcohol if not observed immediately?

Storing the sample in 70% alcohol preserves it for future observation, allowing it to be kept for weeks or months without immediate analysis.

What is the purpose of the hydrolysis step in the preparation process?

The hydrolysis step is intended to break down the intercellular bonds, making it easier to observe the mitotic divisions in the cells.

How long should the root be kept in the oven during the hydrolysis process?

The root should be kept in the oven for 15 minutes at a temperature of 60°C during the hydrolysis process.

What is the function of aceto-orcein as a stain in the preparation process?

Aceto-orcein is used as a stain to color the chromosomes, allowing for optimal absorption of color for clear observation under a microscope.

How long should the stained sample be left to absorb the color before observation?

The stained sample should be left to absorb the color for 15-30 minutes, although the time may vary depending on the condition of the sample.

What should be done to clean the excess stain before observation under a microscope?

The excess stain should be cleaned off with 45% acetic acid, followed by rinsing and blotting with a tissue to prepare the slide for clear microscopic observation.

How can the results of the mitosis observation be preserved for future reference?

The results of the mitosis observation can be preserved by using a glass slide and cover slip, which makes the preparation permanent and allows for repeated observation.