How to Grow Cordyceps Militaris Full Tutorial
Summary
TLDRThis video from Sporn Sprout offers two methods for cultivating the highly valuable Cordyceps militaris mushrooms, renowned for their medicinal properties. The first method involves creating a liquid culture using honey dissolved in warm water, which is then pressure sterilized and inoculated with spores. The substrate and broth are prepared with specific ingredients and cooked in a rice cooker. The mixture is cooled under a laminar flow hood and transferred to a sterilized bin, inoculated, and incubated in darkness. The second method uses jars with a similar substrate but different measurements, followed by pressure sterilization and inoculation. Both methods require careful incubation and lighting to initiate fruiting, with the mushrooms maturing between the 50th and 60th day.
Takeaways
- 🍄 CPS militaris is a highly valuable mushroom due to its medicinal compounds.
- 📊 The process begins with creating a liquid culture using honey dissolved in warm water.
- 🔬 A magnetic stir bar is recommended for mixing the honey water to speed up the process.
- 🔄 The honey water mixture is pressure sterilized at 15 psi for 30 minutes.
- 🌡 After cooling, the sterilized honey water is inoculated with CPS militaris liquid culture.
- 🧪 Liquid culture should be sourced from a reputable supplier like Ryan Paul Gates at Terrestrial Fungi.
- 🌱 The substrate and broth are made by blending water, malt extract, yeast extract, and brown rice.
- 🍚 The brown rice and broth mixture is cooked in an instant rice cooker and then cooled in a laminar flow hood.
- 🧴 The sterilite container is prepared with air holes and wiped down with bleach water and isopropyl alcohol.
- 💉 Liquid culture is added to the cooled rice, and the container is sealed with micropore tape for incubation.
- 💡 After incubation, the mycelium is exposed to light for fruiting, using an LED light strip or under cabinet lights.
- 🕒 The fruiting process takes approximately 14 days to start pinning, with full maturity between the 50th and 60th day.
Q & A
What are the two methods mentioned in the video for growing Cordyceps militaris?
-The two methods mentioned in the video for growing Cordyceps militaris are the bulk bin method and the individual jar method.
Why is Cordyceps militaris considered one of the most expensive mushrooms in the world?
-Cordyceps militaris is considered one of the most expensive mushrooms in the world due to the medicinal compounds it contains, such as nucleotides, polysaccharides, sterols, proteins, amino acids, and polypeptides.
What is the first step in growing Cordyceps militaris according to the video?
-The first step is to create a large amount of liquid culture using honey dissolved in warm water, which is then pressure sterilized.
What is recommended to speed up the process of dissolving honey in water?
-Using a magnetic stir bar is recommended to speed up the process of dissolving honey in water.
At what pressure and duration should the honey water be pressure sterilized?
-The honey water should be pressure sterilized at 15 psi for 30 minutes.
Who is recommended to purchase the liquid culture from for growing Cordyceps militaris?
-The video recommends purchasing the liquid culture from Ryan Paul Gates at Terrestrial Fungi.
What should be done with the master syringe received from Ryan Paul Gates?
-The master syringe received from Ryan Paul Gates should be immediately stored inside the refrigerator until use.
What is the purpose of using a laminar flow hood when handling the substrate and broth?
-The laminar flow hood is used to maintain a sterile environment while handling the substrate and broth, preventing contamination.
How long should the inoculated rice in the bin be incubated in complete darkness?
-The inoculated rice in the bin should be incubated in complete darkness for 3 days at no higher than 70° F.
What is the potential issue that can occur if the substrate is not exposed to lighting after 3 to 4 days of incubation?
-If the substrate is not exposed to lighting after 3 to 4 days of incubation, a problem called 'overlay' can occur, where the mycelium becomes very thick on top of the substrate and prevents any further growth.
What is the recommended temperature range for fruiting Cordyceps militaris?
-The recommended temperature range for fruiting Cordyceps militaris is between 65 and 68° F.
How long does it typically take for the Cordyceps militaris cups to fully mature?
-The Cordyceps militaris cups will be fully mature between the 50th and 60th day after initiating fruiting.
Outlines
🍄 Introduction to Growing Cordyceps Militaris
This paragraph introduces the video's focus on cultivating Cordyceps Militaris, a highly valuable mushroom known for its medicinal properties such as nucleotides, polysaccharides, sterile proteins, amino acids, and polypeptides. The video promises to demonstrate two methods for growing this mushroom: the bulk bin method and the individual jar method. The initial step involves creating a liquid culture using honey dissolved in warm water, which is then pressure sterilized. After cooling, this honey water is inoculated with Cordyceps Militaris liquid culture obtained from Ryan Paul Gates at Terrestrial Fungi. The process also includes using a magnetic stir bar for mixing and incubating the liquid culture in the dark at temperatures not exceeding 70°F.
🌱 Preparing Substrate and Broth for Bulk Bin Method
The second paragraph details the preparation of the substrate and broth for the bulk bin method of growing Cordyceps Militaris. It involves blending water with malt and yeast extracts and then combining this mixture with brown rice in an instant rice cooker. After cooking, the rice is cooled in a laminar flow hood using a sterile spoon. The bin is prepared by poking holes for air exchange, cleaning with bleach water and isopropyl alcohol, and then adding the cooled rice and liquid culture. The bin is covered with micropore tape and incubated in darkness for three days at a temperature not exceeding 70°F. This paragraph also introduces an alternative method for those without a laminar flow hood, involving different measurements for the substrate and broth.
🔬 Sterilization and Inoculation for Individual Jar Method
The final paragraph explains the sterilization and inoculation process for the individual jar method. It includes pressure sterilizing a jar containing a mixture of water, malt extract, yeast extract, and brown rice at 15 psi for 45 minutes. After cooling, the jar is inoculated with liquid culture using a sterile syringe, ensuring the entire surface is covered to prevent excessive mycelium replication. The inoculated jar is then incubated in the dark for three days at a temperature not exceeding 70°F. The paragraph warns against incubating for more than 3 to 4 days to avoid a problem called 'overlay,' where excessive mycelium growth can hinder further development. The process concludes with the use of an LED light strip and a light timer to initiate pinning, with the optimal temperature range being 65 to 68°F. The video ends with a note on the expected maturation period of the mushrooms, which is between the 50th and 60th day.
Mindmap
Keywords
💡Cordyceps militaris
💡Liquid culture
💡Inoculation
💡Substrate
💡Broth
💡Instant rice cooker
💡Laminar flow hood
💡Pressure sterilization
💡Incubation
💡Fring
💡Mycelia
Highlights
Introduction to two methods for growing Cordyceps militaris, a highly valuable medicinal mushroom.
Cordyceps militaris contains important medicinal compounds such as nucleotides, polysaccharides, sterols, proteins, amino acids, and polypeptides.
Creating a liquid culture is the first step in growing Cordyceps militaris.
Using honey dissolved in warm water to create the liquid culture for Cordyceps militaris.
Pressure sterilization of the honey water at 15 psi for 30 minutes.
Inoculating the sterilized honey water with Cordyceps militaris liquid culture.
Recommendation to purchase liquid culture from Ryan Paul Gates at Terrestrial Fungi.
Instructions for storing the master syringe of liquid culture in the refrigerator.
Preparing the substrate and broth by blending water, malt extract, and yeast extract with brown rice.
Cooking the substrate in an instant rice cooker and cooling it in a laminar flow hood.
Preparation of the 16-quart Sterilite container for the bulk bin method.
Incubation of the inoculated rice in complete darkness for 3 days at temperatures not exceeding 70°F.
Alternative individual jar method for those without a laminar flow hood.
Pressure sterilization of the jar with substrate and broth at 15 psi for 45 minutes.
Inoculation of the jar with liquid culture through the self-healing injection port.
Incubation of the inoculated jar in the dark for 3 days at temperatures not exceeding 70°F to prevent 'overlay'.
Initiating fruiting by wrapping an LED light strip around the bin and setting a light schedule.
Maintaining temperatures between 65 and 68°F during the fruiting stage.
Expectation of pinning after about 14 days and full maturity of the cups between the 50th and 60th day.
Transcripts
welcome back to sporn sprout in this
video I'm going to show you two
different ways to grow CPS
militaris first I will show you the bulk
bin method and then I will show you the
individual jar
method CPS militaris is one of the most
expensive mushrooms in the world due to
the medicinal compounds that they
contain nucleotides polysaccharides
steriles proteins amino acids and
polypeptides can be found in Corps
militaris the first step is to create a
large amount of liquid
culture I like to use one tpoon of Honey
dissolved into 600 mL of warm
water using a magnetic stir bar is
recommended to speed up the
process
pressure sterilize the honey water at 15
psi for 30
minutes
after the sterilized honey water has
come to room temperature it is now time
to inoculate it with corp's militarist
liquid culture a breeder will isolate
two ASCO spores and mate them together
to form a new
line I recommend that you purchase your
liquid culture from Ryan Paul Gates at
terrestrial fungi the master syringe you
receive from him should be immediately
stored inside the refrigerator until
use now inoculate your sterilized honey
water with 1 to 2 ml of the liquid
culture
the liquid culture should now be
incubated in the dark at no higher than
70°
f after about 5 days of using the
magnetic stirbar the liquid culture
should look something like like
this once the liquid culture is ready to
be used you can start making the
substrate and
broth start by blending together 1 liter
of water 2 tbsp of light M extract and 2
tbspoon of yeast
extract
add five cups of brown rice and the
broth to an instant rice cooker and use
the brown rice
function
after the cycle has finished you'll have
to shut it off yourself or it will keep
it warm and burn the
bottom while it is still hot open up the
rice cooker in front of a laminar Flo
hood and stir it with a sterile
spoon
remove the insert from the instant rice
cooker and allow it to cool down
completely in front of the laminar flo
hood while the rice is cooling down you
can start prepping your 16q sterilite
container poke a hole in each of the
four corners to allow for fresh air
exchange we will cover these holes after
adding the
rice you will need to wipe down the bin
with bleach water and isopropyl alcohol
because we will not be pressure
sterilizing
it after the rice has cooled down to
room temperature it is now time to add
200 50 mL of liquid
culture
wipe the bin off one last time with
isopropyl alcohol and then add all of
the rice to the bottom in an even
layer
now just cover each hole on the Li with
one layer of micropore
tape this bin will now be incubated in
complete darkness for 3 days at no
higher than 70°
F if you don't have a laminar Flo Hood
this second method is going to be better
suited for
you we will be using the same substrate
in bro bro but just in different
measurements for this batch I don't need
a whole liter of broth so I'm going to
cut it in half and use 500 mL of
water 1 tbspoon of light Mal extract and
1 tbsp of yeast
extract
measure out 35 G of brown
rice
now just add 45 mL of the broth to the
jar of rice not including the
foam
the next step is to pressure sterilize
the jar at 15 psi for 45
minutes
once the jar is cooled down to room
temperature you can now inoculate it
with the liquid
culture
use a sterile syringe to extract the
liquid culture from the
jar
inject the jar through the self-healing
injection port with 10 to 12 mL of the
liquid
culture try to cover the whole surface
to prevent the melium from replicating
excessively the jar will now be
incubated in the dark for 3 days at no
higher than 70° f
it is important not to incubate longer
than 3 to 4 days or you will run into a
problem called
overlay if the substrate is not exposed
to lighting after 3 to 4 days the
mycelium will become very thick on top
of the substrate and it will prevent any
growth the next step is is to wrap an
LED light strip around the bin to
initiate
Fring you should use a light timer to
set the schedule for 12 hours on and 12
hours
off another option would be to use these
under cabinet
lights it is best to keep the
temperatures between 65 and 68° F while
Fring after about 14 days you should
start to see some
pinning the cups will be fully mature
between the 50th and 60th
day and that's all there is to it if you
have any questions leave a comment down
below and don't forget to Subs subscribe
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