How to Cultivate Cordyceps Militaris Mushrooms | With William Padilla-Brown | Grow in Jars or Bins
Summary
TLDRIn this informative video, William Padilla Brown, founder of Microsymbiotics, shares his journey in cultivating Cordyceps militaris mushrooms, a species known for its aphrodisiac and energy-boosting properties. He emphasizes the importance of citizen science and demonstrates a simple, accessible method for growing these mushrooms at home using rice as a substrate and coconut water as a nutrient-rich base. Brown outlines the process from preparing the substrate to sterilization and inoculation, providing practical tips for beginners. He also touches on the potential medicinal benefits of Cordyceps, including its ability to inhibit HIV replication, and encourages viewers to explore holistic medicine and mycology.
Takeaways
- ๐ฑ William Padilla Brown is the founder of Microsymbiotics, an environmental research business based in New Cumberland, Pennsylvania.
- ๐ The business focuses on achieving balance with local ecology and the community, with an emphasis on citizen science and public acts of scientific engagement.
- ๐ Brown discusses cultivating Cordyceps mushrooms, which have health benefits as an aphrodisiac and an energy booster without the crash associated with caffeine.
- ๐ Cordyceps mushrooms can be found at high altitudes and are believed to increase the amount of oxygen in the blood, suggesting potential uses in a new molecular industry.
- ๐พ The cultivation process involves creating a substrate matrix for the mushroom fungus to grow on, which is typically rice enriched with supplements to mimic the mushrooms' natural diet.
- ๐ฅฅ Ingredients for the nutrient broth include coconut water, which provides potassium beneficial for Cordyceps, and tapioca starch as a carbon source for the mushrooms.
- ๐งช Additional supplements such as magnesium sulfate, rock dusts or crushed vitamins, brown rice, soy peptone, kelp powder, and gypsum are used to enrich the growing medium.
- ๐ ๏ธ Brown demonstrates the use of simple tools like a pressure cooker, spoons, a scale, and a whisk to prepare the substrate, emphasizing the accessibility of mushroom cultivation.
- ๐ก๏ธ Sterilization of the substrate is crucial, and Brown uses a pressure cooker to achieve this, recommending at least 10 psi for 45 minutes to an hour and a half.
- ๐งฌ Recent research suggests that two active compounds in Cordyceps militaris, cortisepine and cortimon, have the potential to inhibit HIV-1 reverse transcriptase, highlighting the mushroom's medicinal value.
- ๐ฑ The script also covers the process of inoculating the sterilized substrate with liquid culture and the importance of maintaining proper moisture levels and cleanliness to avoid contamination.
Q & A
What is the name of the business founded by William Padilla Brown?
-The business founded by William Padilla Brown is called Microsymbiotics.
What is the primary focus of Microsymbiotics?
-Microsymbiotics is an environmental research business that focuses on cultivating cordyceps mushrooms and achieving balance with local ecology and community.
What are the two main benefits of cordyceps mentioned in the script?
-The two main benefits of cordyceps mentioned are its use as an aphrodisiac and its ability to provide cellular energy without the crash associated with caffeine.
What role does coconut water play in the cultivation process of cordyceps?
-Coconut water is used as the base of the nutrient broth for cultivating cordyceps because it contains potassium, which is beneficial for the growth of cordyceps.
What is the purpose of using tapioca starch in the cultivation of cordyceps?
-Tapioca starch is used because it breaks down into sugars, which the mushrooms use as a carbon source to create their bodies.
What is the significance of magnesium sulfate in the cultivation process?
-Magnesium sulfate acts as a mineral supplement in the nutrient broth, supporting the growth of cordyceps.
Why is kelp powder used in the cultivation of cordyceps?
-Kelp powder is used for its macro and micronutrients and minerals, which help ensure healthy growth and the production of desired compounds in cordyceps.
What is the purpose of using gypsum in the cultivation process?
-Gypsum is used to add calcium and magnesium, and to provide rigidity and structure to the mushroom, similar to its use in other mushroom cultivation.
What is the recommended sterilization method for the jars used in cultivating cordyceps?
-The jars are sterilized using a pressure cooker, with settings to achieve at least 10 psi for 45 minutes to an hour and a half, or 15 psi for 45 minutes with an All-American pressure cooker.
What are the two active compounds in cordyceps militaris mentioned in the script, and what are they capable of?
-The two active compounds mentioned are cordycepin and cordimon, which are capable of inhibiting HIV-1 reverse transcriptase, preventing the HIV virus from replicating.
What is the recommended method for beginners to start cultivating cordyceps?
-The Instant Pot technique is recommended for beginners as it is easier to get into and focuses solely on cordyceps cultivation.
What is the importance of maintaining the right moisture level in the substrate?
-Maintaining the right moisture level is crucial as too little moisture prevents mycelium growth, while too much saturation can lead to bacterial growth instead of mycelium.
What is the role of the flow hood area in the cultivation process?
-The flow hood area is prepared for introducing the liquid culture to the substrate in a sterile environment, which is essential for successful mycelium growth.
What is the recommended incubation period and temperature for cordyceps?
-The recommended incubation period is about three to six days for the mycelium to grow through the substrate, and the environment should be kept at about 65 degrees Fahrenheit from incubation until harvest.
How can harvested cordyceps be stored or preserved?
-Harvested cordyceps can be stored in ethanol or vinegar for extracts or preservation, or they can be dehydrated and stored in a container with silica packets for later use in teas, foods, or extracts.
What is the most common contaminant encountered in cordyceps cultivation, and what conditions does it favor?
-The most common contaminant is calculosporium corticola, a fungus that lives inside cordyceps and exposes itself when conditions are not ideal, such as when it's too warm, above 68-69 degrees Fahrenheit.
How can one follow William Padilla Brown and Microsymbiotics for more information?
-One can follow William Padilla Brown on Instagram under the handle @michael_senbaio, check out the Microsymbiotics website at michaelsonbiotics.net, and watch educational content on YouTube from Apex Grower.
Outlines
๐ฑ Introduction to Microsymbiotics and Cordyceps Cultivation
William Padilla Brown, founder of Microsymbiotics, introduces his environmental research business based in New Cumberland, Pennsylvania. He discusses his mission to balance local ecology and community, highlighting the cultivation of Cordyceps mushrooms. Brown emphasizes the benefits of Cordyceps as an aphrodisiac and energy booster without the crash associated with caffeine. He also mentions the potential of Cordyceps to increase oxygen levels in the blood and its growing importance in the molecular industry. Brown encourages citizen science and public acts of scientific exploration, sharing his experience as a high school dropout to show that science is approachable. He details the process of preparing a substrate for Cordyceps cultivation, using rice as a matrix and various supplements to mimic the mushroom's natural diet of nutritious bugs. The video provides a simple method to grow Cordyceps at home using an instant pot and basic household items.
๐ Preparing the Nutrient Broth and Substrate for Cordyceps
The video script outlines the process of creating a nutrient broth using coconut water, which is rich in potassium beneficial for Cordyceps. Various ingredients such as tapioca starch, magnesium sulfate, and soy peptone are mixed into the broth to provide a carbon source and necessary minerals for the mushrooms' growth. Additional supplements like kelp powder and gypsum are added for macro and micronutrients and to provide structure to the mushroom. The script also describes the preparation of lids for jars with filtration to ensure proper air exchange for the growing mycelium. The detailed steps include measuring specific amounts of broth and rice for sterilization in a pressure cooker, which is essential for preventing contamination and promoting healthy mycelium growth.
๐ฌ Sterilization and Inoculation Techniques for Cordyceps Cultivation
The script explains the sterilization process using an All-American pressure cooker, highlighting the importance of reaching at least 10 psi for 45 minutes to an hour and a half. It mentions ongoing research into Cordyceps' potential health benefits, such as inhibiting HIV replication. The Instant Pot technique is introduced as an accessible method for beginners, with detailed steps for mixing rice and broth, ensuring accurate measurements, and sterilizing the mixture. After sterilization, the script describes the inoculation process, including the careful introduction of liquid culture into the cooked substrate. It emphasizes the importance of sterility during inoculation to prevent contamination and ensure successful mycelium growth.
๐ผ Cordyceps Harvesting, Storage, and Contamination Prevention
The final paragraph discusses the nuances of harvesting Cordyceps, noting the importance of recognizing the mature stage of the fruiting bodies characterized by the ripening of the perithecium on the stroma. It provides guidance on storing the harvested mushrooms in ethanol, vinegar, or through dehydration for later use in various applications. The script also warns about common contaminants such as Calculosporium corticola and general bacteria and molds, advising on ideal temperature ranges to prevent their growth. Additionally, it touches on the rare issue of mites in culturing and suggests resources for further information, including Instagram, a festival called MicroFest, and Patreon for those interested in supporting the work.
Mindmap
Keywords
๐กMicrosymbiotics
๐กCordyceps
๐กCitizen Science
๐กMycelium
๐กSubstrate
๐กInstant Pot
๐กNutrient Broth
๐กMineral Supplementation
๐กFiltration
๐กInoculation
๐กHarvesting
๐กContamination
Highlights
Microsymbiotics is an environmental research business focused on achieving balance with local ecology and community.
William Padilla Brown discusses the dual benefits of cordyceps as an aphrodisiac and energy booster without the caffeine crash.
Cordyceps can be found at high altitudes and are known to increase oxygen levels in the blood.
The potential of cordyceps in the emerging molecular industry is highlighted.
Citizen science is encouraged as an approachable field with significant potential for discovery.
A simple method for cultivating cordyceps using rice as a solid-state matrix is introduced.
The use of coconut water in nutrient broth is explained for its potassium benefits.
Tapioca starch is essential as it breaks down into sugars used by mushrooms for growth.
Magnesium sulfate and various supplements are used to enrich the growth matrix for cordyceps.
Gypsum is used to add calcium, magnesium, and structure to the mushroom growth.
Polyester fiber is used as a filter for jars to allow for cordyceps growth and culture introduction.
The importance of sterilization and maintaining a clean environment for mycelium growth is emphasized.
Cordyceps militarists' cortisepine and cortimon are identified as compounds with potential HIV inhibitory properties.
The instant pot technique is presented as an accessible method for beginners to cultivate cordyceps.
Accurate measurement of rice and broth is crucial for substrate preparation.
Proper distribution of liquid culture in the substrate ensures even mycelium growth.
Incubation should be in the dark to prevent photosensitivity in cordyceps mycelium.
Harvesting cordyceps requires knowledge of the strain and recognizing maturity in the fruiting bodies.
Common contaminants and pests in cordyceps cultivation are identified and methods to avoid them are suggested.
Resources for further information on mycology and cordyceps cultivation are provided.
Transcripts
[Music]
i'm the founder of microsymbiotics
microsymbiotics is an environmental
research business based in new
cumberland pennsylvania that i started
in 2015 i made it one of my priorities
to
achieve some sort of balance with my
local ecology and
the community around me hey everybody
it's william padilla brown from
microsymbiotics coming at you with the
second harvest from our commercial
quarter steps militarist grow here
um cordyceps lends itself in two
desirable ways to humanity as an
aphrodisiac and then for the energy on a
cellular level that doesn't have that
same crash like caffeine does and also
they can be found in high altitudes i
found
cordyceps at some of the highest
altitudes in north america
and they can increase the amount of
oxygen that's in our blood but i think
as more people start to understand it's
its role i think that uh
it'll have all sorts of use in a new
world of a molecular industry
i just think that it's important to
encourage citizen science and you know
public acts of citizen science i don't
know i think i'm just a good
representation as like a high school
dropout like brown skinned person doing
science shows that it's really
approachable and the more people that
are doing it the more that we're going
to uncover the more research that we're
going to find and the more practical
applications of science that we're going
to find
today we're going to be preparing
substrate for cultivating cordyceps
mushrooms basically what we're doing is
we're creating a matrix something for
the mushroom fungus to grow on which is
the rice the rice is a solid state
matrix it's something for the mycelium
to live on and then we're enriching that
matrix with supplements you know because
cordyceps grow in the wild on nutritious
bugs so we kind of want to recreate that
nutrition for them so they can produce
all those beautiful compounds that we
like
and it doesn't really take a big fancy
lab or anything like that whenever i
first learned how to grow it i was just
doing it in like a box in my house
there's information into tutorials
online on how to set everything up with
just a uh instant pot so i mean really
with the small investment of an instant
pot and a clean space in your house you
can start doing everything that we're
doing right now
we're going to start with a nutrient
broth and the base of our nutrient broth
is going to be coconut water today
a lot of times we'll use water
but coconut water has potassium in it
that is also very beneficial for
cordyceps so we're just going to rock
out with some of this coconut water
today one of the other very important
ingredients we're going to need is some
tapioca starch the starch is really
important
it breaks down into sugars which the
mushrooms then use as a carbon source to
actually create
their bodies we have some magnesium
sulfate which just acts as a mineral
supplement
and for the rest of our mineral
supplementation
sometimes i'll use rock dusts
but you can totally use crushed one a
day vitamins
we have our brown rice to act as a
matrix for our mycelium to grow on
and we have soy peptone so soy peptone
and nutritional yeast can be used in
replacement for one another um
more labs may have access to soy peptone
this isn't something that you can just
go find at a grocery store where you
could find nutritional use at a grocery
store we have some kelp powder here
this is great for macro micronutrients
and minerals
so this is great for mineral supplement
macronutrients micronutrients and this
just helps to make sure that our
cordyceps grow healthy and produce all
the compounds that we want from them
last but not least we have some gypsum
we use this for the same reason we use
gypsum for all other mushrooms to add a
little bit of calcium magnesium and add
a little bit of rigidity and structure
to the mushroom
[Music]
here we have some of the tools of the
trade
we've got some polyfill we're going to
utilize this for making some filters on
our lids for our jars which we will be
utilizing for fruiting some of the
mushrooms
we have a nice little spoon here for
aiding and mixing things a smaller spoon
for measurement
uh we have some culture here we're going
to use that later
um and
got a nice little scale little skis here
for measuring out our
dry materials that we're going to be
adding into our broth
and then we have a nice little scale
scoop for measuring out our materials
for our broth like a nice little vessel
for mixing
and a tool for mixing as well like this
whisk here um you could also you could
also uh use a blender put all of your
dry materials after you weigh them into
a blender with your liquid blend it up
and then use it like that um but you
know this
gives you a nice feel for it i mean if
you want to hit him with some of that
biodynamic a little mix a little to the
left mix a little to the right
underneath the moonlight you know that's
the way to do it
all right let's get into it
[Music]
i'm going to start
introducing the dry ingredients and
mixing them up into solution with the
coconut water
so i'm going to put the dry ingredients
in here first
[Music]
all right guys so we got all of our dry
ingredients here and we're going to go
ahead and start adding in this coconut
water
[Music]
get everything unstuck from the bottom
there
there we go
upon the weird powders
all right guys if you have any um pesky
clumps in there you might want to grab
an immersion blender or instead of using
a whisk you can just invest in an
immersion blender for your setup
[Music]
that's fun
i'm not even in front i was doing that
longer because i was having fun with it
[Music]
[Music]
all right guys we are going to prepare
some lids here for uh filtration um so
one of the things that i would like to
note is that whatever your hole size
that you're going to drill into your lid
should be big enough for your needle to
go in that you're going to be utilizing
when you inoculate your substrate so i
like to use an 18 gauge one and a half
inch needle so you're going to want to
make sure that whatever you're drilling
into your lid is at least bigger than 18
gauges
[Music]
simple as that
[Music]
and we're going to take a little bit of
this polyester fiber um one of the
tricks that i've learned over the years
is that if you get acrylic fiber yarn
you can take a crochet hook
and you can pull it through more than
one of these lids at once with a crochet
hook you might even be able to take the
polyester fiber but the yarn
you're able to get a nice solid piece
through
more than one at once so if you're going
to be making a lot of these
you might want to try the acrylic yarn
it works just as well as the polyester
fiber
there you go and all you need is just a
little bit of that to get nice and tight
through there
and we have a nice little filter
for our cordyceps to breathe and we can
also introduce our culture through there
[Music]
all right guys we're uh measuring out 46
mils
of uh broth here
and we're going to measure out 28 grams
of rice per pint jar
[Music]
and that's ready to go right into the
pressure cooker
all right we're going to load up our
jars
into the
insert for the all-american
we're going to be using an all-american
today but you can use whatever pressure
cooker you have at home whenever i
started out i started with a presto
you're going to want to make sure that
you can at least get 10 psi that means
pounds per square inch
you can look that up online what your
pressure cooker's capabilities are but
you're going to want at least 10 psi for
45 minutes to an hour and a half
if you're getting contaminations you're
going to want the longer times with the
all-american because we can go to 15 psi
i'm gonna run this for 45 minutes and
we're gonna get this thing loaded up
[Music]
there's research right now that is you
know what i believe to be pretty cutting
edge that shows um two active compounds
and quadriceps militarists cortisepine
and cortimon
are capable of inhibiting hiv one
reverse transcriptase
for a natural product to be able to
inhibit the hiv virus from replicating
its empower is incredibly powerful and i
think that there's going to be a lot
more eyes looking at it and i think that
as we have more eyes looking at it and
as we start to develop the analytical
protocol for it we're going to start to
discover a host of other compounds in it
you know i'm really excited to see where
holistic medicine goes where we start to
when we start to realize that every
single body is different and treat
everybody different instead of giving
everybody the same pills in every body
the same dose for the same medicines
when everybody is different
[Music]
so one of the methods we're going to run
is the instant pot technique
which is way easier for beginners to get
into if you haven't practiced any other
kind of mycology or if you just want to
focus on cordyceps only instapod is a
great technique to lock into so we're
going to separate a liter
or a thousand mils by separating two
500s out and
putting it into our insta pot all right
guys uh we have here the insert for our
insta pot so we're going to mix the
things that we're gonna are gonna go in
there into here um and all that's gonna
go in there is our rice and our broth
[Music]
all right and it's important to weigh
the rice out and make sure the
measurements are accurate um when you're
preparing your quarter step substrate
because
too much rice or too much liquid can end
up with your substrate not having enough
moisture or being too saturated not
having enough moisture your mycelium
won't grow too saturated bacteria will
grow
[Music]
all right guys we're just gonna set this
in the instant pot
and uh
run it for the right setting
[Music]
so um we have our instant pot all done
and the
all-american is all done sterilizing so
we're gonna go prep the flow hood area
in preparations for
introducing our liquid culture to our
substrate
[Music]
all right guys so
we have our jars uh sterilized and
cooled down
and we're gonna take them out
of the
pressure cooker insert here
and you see the rice is nice and cooked
there is a little bit of moisture in
there but that's okay you don't want too
much standing water but you want a
little bit of excess moisture
which is going to provide the mushrooms
with their humidity inside of the jar as
they grow
when you receive your culture you're
going to want to remove the cap
and quickly attach your sterile needle
and when you first open your needle it's
sterile so you can go directly
into a jar
and you're going to want to introduce
about one to two mils in a circular
rotation through your jar
then remove the needle
and set that aside
um you can sterilize your needle in a
back incinerator or with a flame i'm
going to go ahead and stick it in the
back generator and now that it's sterile
i can go ahead and insert it in through
one of these injection ports here
and the same introduce two mils
and circular rotation and this allows
the mycelium to be distributed so you
can see it grow out in a broader
fashion
[Music]
all right for the amount of substrate
that we made in the instapot i'd
recommend at least 120 mils
at most 300 mils of liquid culture
and then you can mix up your
liquid culture into the rice
[Music]
all right i'm gonna clean up my spoon
here i'm gonna make sure you got like a
stainless steel or something like that
and clean it up with this uh diluted
ethanol
or isopropyl
and make sure that uh that your solution
is dried up
your sterilization solution your ethanol
or isopropyl is dry before you stick it
in there to mix it up so that you're not
killing your mycelium
now we're just mixing our liquid culture
in
[Music]
we don't need uh too big of a layer like
no more than a half inch
if you pile it in too much
you're gonna be uh reducing the amount
of space you have
for your fruits to grow so if you have a
culture that has really tall fruiting
bodies and you put too much rice they're
not going to be able to grow as much in
there
so
because this one doesn't have a rubber
seal
but it does have these clips
i'm just going to let it clipped
and
put it in dark incubation if you have a
rubber seal
what i would recommend doing is putting
a hole and putting a filter patch on it
if you have
a tub that doesn't have clamps that just
has a lid that clicks on
i would recommend not using that at all
because there's going to be too much
airflow if you run your whatever bins
that you get if you run them and you're
noticing that it's drying out
you may want to try
putting a line of tape to kind of create
your own gasket or maybe search for one
that has a gasket um
i've had plenty of success with just
putting a line of tape around and no
filter so yeah we're going to set this
in a dark space to incubate over the
next three days
incubation should be in the dark
cordyceps are very photosensitive and
they'll start to change the color of
their mycelium when exposed to light
generally with a healthy culture it'll
take about three to six days for the
mycelium to grow through the substrate
and then after that you will introduce
it to light and you're going to want to
keep the environment about 65 degrees
from incubation time until harvest
[Music]
harvested in cordyceps is like a little
nuanced um you're going to have to like
do it a couple of times and know your
strain because it's going to produce
parathesium on the top of the stroma
perithesium
are technically the fruiting body and
the stroma is like
means of the fruiting body not being in
the dirt like the straw like the the
little
finger looking cordyceps thing that you
see there's bumps on the top of it those
bumps are the fruits the whole finger
thing is just a
fungal stretch
of fungal material for the fruiting
bodies to be in the air
so when those come out and though those
get mature that's when it's ready to
harvest after those are mature then it
starts to die so like you're looking for
the little bumps on the top to get ripe
which is like takes a while to know what
you're looking at you can store them in
ethanol
for you know extracts or just for
preservation
you can store them in vinegar you know
for extracts or just for preservation
where you can dehydrate them
and you know just store them in a
container in a dark with silica packets
and then make teas or foods or
extracts or whatever
[Music]
the most common contaminant is going to
be calculosporium corticola which is
some sort of fungus that lives inside of
the cordyceps and exposes itself when
the conditions aren't ideal so usually
when it's too warm um anything like
above like 68 69
for any long extended period of time
you'll probably start to see this white
fuzz growing on top of your orange
mycelium or growing on top of your
cordyceps
so that's the most common thing and then
just general bacteria and molds that
you're going to be encountering in any
kind of mycology
and mites
if you get mites they like to go in and
out of the jars and eat your mycelium
so you'll see patches in the mycelium
mites aren't common if you're not doing
wild culturing if you're just growing
only fruiting bodies in lab and stuff
like that you might never ever get mites
i definitely encourage people to follow
me on instagram that's where i post a
lot of really cool information that's
michael senbaio you can also check out
microsymbiotics
microfest every august first weekend of
august it's our festival that we host
that's a lot of fun for people to get
engaged with
michaelsonbiotics.net has good
information apex grower on youtube has a
lot of good information and if anybody
wants to support us monetarily
i'm on patreon as permaculture poppy or
you can check the link in my bio on my
instagram just for general donations all
the links are down below let's go
[Music]
you
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