Pengamatan Mitosis Pada Akar Bawang dengan Metode Squash

Scorbia 21
19 Jul 202006:17

Summary

TLDRIn this educational video, viewers are guided through the process of observing mitosis in onion root tips. The tutorial begins with cultivating the roots in a damp environment or water to encourage growth. The roots are then treated with a 0.02% colchicine solution to inhibit spindle formation and enlarge chromosomes. Afterward, a Carnoy's solution is used for cell fixation. The video covers the preparation steps, including hydrolysis and staining with aceto-orcein, to reveal the chromosomes. Finally, the observer can examine the different stages of mitosis—prophase, metaphase, anaphase, and telophase—under a microscope. The video concludes with tips for preserving the samples for future observation.

Takeaways

  • 🧬 The video script discusses observing mitosis in onion root tips.
  • 🌱 The first step is to grow onion roots by placing them in a moist area or submerging them in water.
  • 🔪 The bottom part of the onion is cleaned to ensure quick root growth.
  • 💧 The root tips are then placed in a 0.02% colchicine solution for 1-2 hours to inhibit spindle formation and enlarge chromosomes.
  • 🧪 After colchicine treatment, the solution is replaced with Carnoy's solution for 2-3 hours to fix and preserve the cell condition.
  • 🚫 If immediate observation is not needed, the sample is stored in 70% alcohol for preservation.
  • 🔬 The purpose of the treatment is to break down cell walls to facilitate the observation of mitotic division.
  • 🔥 The sample is then hydrolyzed in a reaction tube at 60°C for 15 minutes, followed by rinsing and staining with 2% aceto-orcein.
  • 🎨 The staining process takes 15-30 minutes or more, depending on the sample condition.
  • 🔍 After staining, excess stain is washed off with 45% acetic acid, and the slide is prepared for observation under a microscope.
  • 📸 The video suggests using a 100x objective lens for observation and applying immersion oil to the cover glass for clarity.
  • 🖼️ The results of the mitosis observation can be preserved using a glass slide and a cover slip for permanent and repeatable viewing.

Q & A

  • What is the main topic of the video script?

    -The main topic of the video script is learning how to observe mitosis by studying the root tips of an onion.

  • How should the onion root be prepared for mitosis observation?

    -The onion root should be prepared by placing it in a damp area or submerging it in water to encourage root growth. The bottom part of the onion should be cleaned to facilitate quick root development.

  • What is the purpose of using a 0.02% colchicine solution in the process?

    -The 0.02% colchicine solution is used to inhibit spindle fiber formation during cell division and to enlarge the chromosomes for easier observation.

  • What is the role of Carnoy's solution in the preparation process?

    -Carnoy's solution is used to fix or preserve the condition of the cells after colchicine treatment, ensuring the cells are kept in a stable state for observation.

  • Why is it necessary to store the sample in 70% alcohol if not observed immediately?

    -Storing the sample in 70% alcohol preserves it for future observation, allowing it to be kept for weeks or months without immediate analysis.

  • What is the purpose of the hydrolysis step in the preparation process?

    -The hydrolysis step is intended to break down the intercellular bonds, making it easier to observe the mitotic divisions in the cells.

  • How long should the root be kept in the oven during the hydrolysis process?

    -The root should be kept in the oven for 15 minutes at a temperature of 60°C during the hydrolysis process.

  • What is the function of aceto-orcein as a stain in the preparation process?

    -Aceto-orcein is used as a stain to color the chromosomes, allowing for optimal absorption of color for clear observation under a microscope.

  • How long should the stained sample be left to absorb the color before observation?

    -The stained sample should be left to absorb the color for 15-30 minutes, although the time may vary depending on the condition of the sample.

  • What should be done to clean the excess stain before observation under a microscope?

    -The excess stain should be cleaned off with 45% acetic acid, followed by rinsing and blotting with a tissue to prepare the slide for clear microscopic observation.

  • How can the results of the mitosis observation be preserved for future reference?

    -The results of the mitosis observation can be preserved by using a glass slide and cover slip, which makes the preparation permanent and allows for repeated observation.

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相关标签
MitosisLab TechniquesOnion Root TipsCell DivisionBiology EducationMicroscopyChromosome CountLab ProtocolEducational VideoScience Experiment
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