PRAKTIKUM ENZIM KATALASE pada HATI AYAM (BIOLOGI KLS XII)

Summary

In this educational video, the instructor guides students through a practical experiment on the enzyme catalase, focusing on factors affecting its activity. Using blended chicken liver, the experiment tests the enzyme's reaction to various pH levels and temperatures by observing the breakdown of hydrogen peroxide into water and oxygen. The presence of oxygen is confirmed by a glowing ember. The video highlights the enzyme's efficiency in neutral conditions and its inactivity under acidic conditions or high temperatures, emphasizing the importance of liver function in detoxifying the body.

Takeaways

• 🧪 This practical session is about the enzyme catalase, part of the metabolic process discussed in Chapter 2.
• 🦴 The experiment will test factors affecting catalase activity found in the liver.
• 🍗 Materials used include blended chicken liver, distilled water, HCl, H2O2, test tubes, test tube clamps, dropper pipettes, a Bunsen burner, and wooden sticks.
• 🌡️ The first test involves adding 20 drops of chicken liver solution to a neutral pH solution at room temperature.
• 🧴 The second test uses a basic pH solution by adding 5 drops of NaOH, mixed with liver solution.
• 🥽 The third test involves an acidic pH solution by adding 5 drops of HCl to the liver solution.
• 🔥 The fourth test heats the liver solution to high temperatures until boiling.
• 🫧 H2O2 is added to each test tube to observe the reaction, indicated by bubble formation.
• 🧯 Oxygen production is tested by seeing if a glowing stick ignites in the presence of bubbles.
• 💡 The results show catalase activity: effective in neutral and basic pH, but not in acidic pH or high temperatures.

Q & A

What is the main topic of the practical lesson discussed in the script?

The main topic of the practical lesson is testing the factors that affect the activity of the enzyme catalase.

What are the primary materials used in the experiment?

The primary materials used in the experiment include blended chicken liver, distilled water, HCl, H2O2, test tubes, test tube holders, pipettes, a Bunsen burner, and wooden splints.

What is the purpose of blending the chicken liver in the experiment?

Blending the chicken liver is done to obtain a homogenized mixture that contains the enzyme catalase.

What role does H2O2 (hydrogen peroxide) play in the experiment?

H2O2 is used to test the activity of the catalase enzyme. Catalase breaks down H2O2 into water and oxygen.

How is the effect of pH on catalase activity tested?

The effect of pH on catalase activity is tested by adding NaOH to create a basic solution and HCl to create an acidic solution, and then observing the enzyme activity in these different pH conditions.

What observation indicates that oxygen is being produced in the reaction?

The production of bubbles in the reaction indicates that oxygen is being produced, and this can be further confirmed by a glowing splint test, where the splint reignites in the presence of oxygen.

How does temperature affect the activity of catalase in this experiment?

The experiment tests the effect of temperature by heating one of the test tubes containing the liver mixture. Heating denatures the enzyme, preventing it from breaking down H2O2, which is indicated by the absence of bubbles.

What conclusion can be drawn about the enzyme catalase's activity at different pH levels?

Catalase is active at a neutral pH but becomes inactive in highly acidic or basic conditions.

What is the practical implication of catalase activity in the human body mentioned in the script?

The practical implication is that catalase helps detoxify hydrogen peroxide, a harmful byproduct of metabolism, by converting it into water and oxygen.

What does the script suggest about the stability of catalase at high temperatures?

The script suggests that catalase becomes denatured and inactive at high temperatures, as indicated by the lack of bubbles when the heated liver mixture is tested with H2O2.