METABOLISME : Praktkikum Uji Enzim Katalase (pengaruh PH dan Suhu terhadap kerja Enzim)

Summary

In this educational video, Pak Dudi explains the factors influencing the activity of the enzyme catalase, focusing on temperature and pH. Through a practical experiment, students observe how catalase reacts in different conditions, using hydrogen peroxide and various pH levels (acidic, neutral, and basic). The experiment also tests the enzyme's response to heat. Results show that catalase works best at neutral pH and optimal temperatures. The conclusion emphasizes how both pH and temperature impact the enzyme's effectiveness in neutralizing toxins in the body, with significant changes noted when conditions are altered.

Takeaways

• 😀 Enzyme catalase neutralizes toxins in the body, breaking down hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2).
• 😀 Catalase activity is influenced by various factors like pH and temperature, which are tested in the experiment.
• 😀 The experiment uses chicken liver as a source of catalase and tests the enzyme under different conditions.
• 😀 The first experiment tests the effect of hydrogen peroxide (H2O2) alone, which produces oxygen bubbles and proves that catalase is active.
• 😀 To test the presence of oxygen, a lit splint is introduced to check for ignition, confirming that the gas is indeed oxygen.
• 😀 The second experiment tests the enzyme's activity in acidic conditions using hydrochloric acid (HCl), which results in less bubble formation.
• 😀 The third experiment tests the enzyme in basic conditions using sodium hydroxide (NaOH), where no bubbles form, indicating catalase is inactive in this environment.
• 😀 The fourth experiment tests the effect of heat on catalase by heating the enzyme mixture, which prevents catalase from working effectively.
• 😀 The results of the experiments show that catalase works best in neutral pH and optimal temperatures, with its activity reduced under extreme pH levels and high heat.
• 😀 The session concludes with the importance of understanding how pH and temperature affect enzymatic reactions, with catalase being most active at neutral pH and normal body temperature.

Q & A

What is the main objective of the lesson in this transcript?

The main objective of the lesson is to study the factors that influence the activity of the enzyme catalase, particularly focusing on how pH and temperature affect its function.

What is catalase, and where is it found?

Catalase is an enzyme found in the liver. Its primary function is to neutralize toxins in the body, specifically by breaking down hydrogen peroxide into water and oxygen.

What chemical reaction does catalase facilitate?

Catalase facilitates the breakdown of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). This reaction helps detoxify cells by neutralizing the harmful effects of hydrogen peroxide.

What materials were prepared for the experiment on catalase?

The materials prepared for the experiment included test tubes, a spirit lamp for heating, a mortar and pestle for crushing, chicken liver, hydrogen peroxide (H2O2), sodium hydroxide (NaOH), hydrochloric acid (HCl), and a glass rod.

How is the experiment conducted to test the effect of temperature on catalase activity?

To test the effect of temperature, the liver extract in a test tube is heated using a spirit lamp to observe whether heat affects the formation of oxygen bubbles, indicating catalase activity. The results show that heating the enzyme impairs its function.

What happens when catalase is exposed to different pH levels in the experiment?

The experiment demonstrates that catalase works best at a neutral pH. When exposed to acidic (HCl) or basic (NaOH) conditions, the enzyme's ability to break down hydrogen peroxide is significantly reduced, as shown by fewer oxygen bubbles and no flame when tested with a burning stick.

What was observed when the catalase was tested at neutral pH with hydrogen peroxide?

When catalase was tested at neutral pH with hydrogen peroxide, a significant reaction occurred, producing many oxygen bubbles. When a burning stick was inserted, the flame was brightened, confirming the presence of oxygen.

What was the effect of an acidic environment on catalase activity in the experiment?

In an acidic environment (after adding HCl), catalase activity was reduced. While some oxygen bubbles formed, they were fewer in number, and the burning stick did not light up, indicating that the enzyme's activity was impaired by the acidic pH.

What does the absence of oxygen bubbles in the experiment indicate?

The absence of oxygen bubbles suggests that catalase is not functioning properly. This could be due to factors like extreme pH (acidic or basic) or high temperatures that denature the enzyme, preventing it from breaking down hydrogen peroxide.

What conclusion can be drawn from the experiment regarding the factors that affect catalase activity?

The conclusion of the experiment is that catalase works optimally at a neutral pH and at an optimum temperature. Both extremely acidic (HCl) and basic (NaOH) conditions, as well as high temperatures, reduce or completely inhibit catalase's ability to function.