TUTORIAL PENGGUNAAN AUTOKLAF & OVEN DALAM PROSES STERILISASI ALAT (Basah & Kering)

Summary

This video demonstrates the process of sterilizing laboratory equipment using an autoclave and an oven. The autoclave sterilizes items by applying heat to destroy resistant bacteria, while the oven sterilizes heat-resistant tools at 140°C. The autoclave process includes careful packaging, water management, precise temperature and time settings, and the safe removal of sterilized items. The oven method involves setting a temperature of 140°C and a 3-hour duration. Both methods ensure that equipment is free from microorganisms and ready for use. The video emphasizes safety precautions and proper handling of hot equipment throughout the process.

Takeaways

• 😀 Proper packaging of tools is essential before sterilization to ensure they remain sterile and prevent contamination or breakage.
• 😀 Autoclave sterilization is used to eliminate heat-resistant bacterial spores, ensuring sterilization of tools.
• 😀 Autoclaves are closed heating devices designed to sterilize equipment and finished preparations.
• 😀 Before sterilization, the autoclave should be checked for sufficient water levels and additional water must be added if necessary.
• 😀 After placing all the tools inside the autoclave, ensure the lid is properly sealed to avoid steam leakage during the sterilization process.
• 😀 The autoclave should be set to a temperature of 121°C and a sterilization time of 30 minutes for effective results.
• 😀 After the sterilization cycle is complete, the autoclave should be turned off, and steam should be released carefully to avoid burns.
• 😀 Tools should be handled with care as they remain hot after being sterilized in the autoclave.
• 😀 Sterilization using an oven is done at 140°C for tools that are heat-resistant, and the process typically takes 3 hours.
• 😀 It's important to set both the temperature and time properly on the oven to ensure effective sterilization of the equipment.
• 😀 Once sterilization is complete, remove the tools carefully, allow the oven to cool down, and ensure it is turned off after use.

Q & A

Why is packaging important before sterilizing the tools?

Packaging is important because it ensures the tools remain sterile until used and helps prevent breakage or contamination when the tools are removed from the autoclave.

What is the purpose of using an autoclave for sterilization?

The purpose of using an autoclave is to kill bacterial endospores that are resistant to heat, drying, and antibiotics, ensuring the sterilization of tools and materials.

How should the autoclave be prepared before use?

Before use, check the water level inside the autoclave, add water up to the indicated limit, insert the racks and filter, and then place the items to be sterilized inside the autoclave.

What are the main components inside the autoclave?

Inside the autoclave, there are a container, a metal filter, and a support rack to keep the items from touching the water directly.

What temperature and time settings are used for sterilizing tools in the autoclave?

The autoclave is set to 121°C for sterilization, and the process lasts for 30 minutes.

How is the autoclave operated to begin the sterilization process?

To begin the process, the autoclave is connected to a power source, turned on using the on/off button, and the temperature and time are set using the control buttons. The autoclave should be sealed tightly to prevent steam from escaping.

How should the autoclave be handled after the sterilization process is completed?

After sterilization, the autoclave is turned off, and the pressure valve is opened to release steam. The autoclave should be unlocked carefully, and the sterilized tools should be removed while they are still hot.

What is the role of the oven in sterilization?

The oven is used to sterilize heat-resistant tools by applying dry heat. It is set to 140°C for 3 hours to ensure thorough sterilization.

How is the oven prepared for use in sterilization?

The oven is connected to a power source, and the temperature and time settings are adjusted on the control panel. The tools are then placed inside the oven, and it is sealed tightly before starting the sterilization process.

What should be done after the sterilization process in the oven is complete?

Once the sterilization is finished, wait for the oven to cool down, open it carefully, and remove the sterilized items. The oven should be turned off and disconnected from the power source.