Mikrobiologi Farmasi: Pengenalan alat laboratorium mikrobiologi

Summary

This transcript outlines a laboratory practical session focused on the introduction to several key laboratory instruments. It covers the usage and functions of a laminar air flow unit, which maintains a sterile workspace, and explains the operation of an oven and autoclave for sterilizing equipment. The video details procedures for maintaining sterility, adjusting temperature and time settings on the oven, and effectively using an autoclave for sterilizing media and tools in microbiological experiments. It provides clear instructions for handling these instruments safely and efficiently in a laboratory setting.

Takeaways

• 😀 Laminar Air Flow (LAF) is used in laboratory settings to maintain a sterile environment by filtering and circulating air to eliminate dust and spores.
• 😀 LAF operates by blowing air through multiple filters, starting with a pre-filter followed by a high-efficiency filter (HEPA), ensuring the workspace remains clean and sterile.
• 😀 Key components of LAF include an on/off switch, blower speed control, UV light switch for sterilization, and an LED display for operational monitoring.
• 😀 UV light in LAF should be used both before and after working to ensure the workspace is sterile and free of bacteria.
• 😀 Before using LAF, the workspace and tools should be cleaned with 70% alcohol to reduce bacterial contamination.
• 😀 In oven usage, temperature and time are adjustable via a touchscreen display, and samples should be placed inside once the settings are confirmed.
• 😀 The oven operates by converting electricity into heat energy, and temperature and time adjustments help control the heating process during experiments.
• 😀 The autoclave is essential in microbiology labs for sterilizing tools and media by using high-pressure steam.
• 😀 Autoclave operation involves connecting to a power source, setting temperature (121°C), and time (15 minutes) for effective sterilization.
• 😀 After sterilization, once pressure drops to normal, the autoclave should be turned off, and the sterilized materials should be carefully removed after cooling.

Q & A

What is the purpose of a laminar air flow (LSF)?

The purpose of a laminar air flow (LSF) is to create a sterile working environment by filtering the air and removing dust and spores, preventing contamination in the workspace during microbiological procedures.

How does a laminar air flow system work?

The laminar air flow system works by drawing in air from the outside or room and passing it through multiple filters, including a primary filter and a secondary high-efficiency filter (HEPA filter), before it is blown into the workspace to ensure sterile conditions.

What are the main functions of the buttons on a laminar air flow unit?

The main functions of the buttons on a laminar air flow unit include turning the system on, adjusting the blower speed, controlling the lights (including UV light), and ensuring the system is operating in a sterile environment before and after usage.

Why is UV light used in a laminar air flow system?

UV light is used in a laminar air flow system to sterilize the workspace before and after usage, ensuring that the environment is free from bacteria and other microorganisms.

What is the first step in using the laminar air flow for microbiological work?

The first step is to clean the workspace using 70% alcohol and a tissue in a specific direction to avoid re-contaminating the surface with bacteria.

What is the purpose of an oven in microbiological work?

An oven is used to apply heat for sterilization purposes, as it works by converting electrical energy into heat energy through conduction to disinfect samples.

How do you set the temperature and time on the oven?

To set the temperature, you adjust the temperature knob to the desired level. To set the time, you use the time knob, rotating it to increase or decrease the time as needed.

How does an autoclave work in sterilization?

An autoclave works by converting electrical energy into heat, which heats water inside the machine to produce steam, raising the pressure and temperature to sterilize equipment and materials.

What is the proper procedure for using an autoclave?

First, connect the autoclave to a power source and turn it on. Then, fill the autoclave with water up to the marked level, insert the items to be sterilized, close the lid securely, set the temperature to 121°C and the time to 15 minutes, and start the autoclave.

What should you do after the autoclave process is complete?

After the sterilization process is complete, wait for the pressure to normalize, then open the autoclave, remove the sterilized materials, and ensure everything has cooled down before turning the autoclave off and disconnecting it from the power source.