MIKROBIOLOGI - STERILISASI

Summary

This microbiology experiment focuses on sterilizing laboratory equipment and preparing microbiological media. The process includes washing, drying, and wrapping tools with cotton and aluminum foil before sterilizing them in an autoclave. Media such as nutrient agar and broth are carefully measured, mixed, and sterilized to ensure proper conditions for microbiological testing. The equipment and media are labeled and stored for future use, with observations made over 24 hours to monitor their condition. The method ensures sterile conditions necessary for accurate microbiological experiments.

Takeaways

• 😀 Sterilization of tools and materials is a key step in microbiological testing.
• 😀 Various tools used in the experiment include pipettes, beakers, petri dishes, chemical needles, and stirring rods.
• 😀 Materials to be sterilized include aluminum foil, nutrient agar, nutrient broth, and cotton.
• 😀 All tools must be washed before sterilization, followed by drying.
• 😀 Glass tools with openings (e.g., beakers, test tubes) must be sealed using cotton wool and cloth.
• 😀 The sealing process involves packing the cotton wool tightly and securing it with thread, then wrapping with cloth.
• 😀 Petri dishes are fully wrapped with paper to ensure sterility.
• 😀 After sealing with cotton wool, tools like test tubes and beakers are wrapped in aluminum foil and tied with thread.
• 😀 The wrapped tools are then placed in an autoclave for sterilization at 121°C for 15 minutes.
• 😀 For media preparation, the required amounts of nutrient agar and broth are calculated and weighed carefully.
• 😀 The nutrient agar and broth are dissolved in distilled water, heated, and mixed with a magnetic stirrer to form a clear solution.
• 😀 After sterilization, the media is cooled, labeled, and stored in a refrigerator for later use.

Q & A

What is the first step in sterilizing the tools and materials for microbiology experiments?

The first step is to wash the tools and materials thoroughly before sterilizing them.

What are some of the tools used in the sterilization process in the microbiology experiment?

The tools used include pipets, measuring cylinders, petri dishes, chemical needles, stirring rods, droplet pipets, and test tubes.

How are the tools like measuring cylinders and test tubes prepared for sterilization?

Tools with openings, like measuring cylinders and test tubes, are closed with cotton balls, then wrapped with gauze and tied with cotton thread before sterilization.

What material is used to seal the mouths of glassware like Erlenmeyer flasks during sterilization?

The mouths of glassware are sealed with cotton balls, and they are further wrapped with gauze and tied with cotton thread.

How are petri dishes prepared for sterilization?

Petri dishes are fully wrapped with paper to prepare them for sterilization.

What is the purpose of wrapping the tools with aluminum foil after sealing them with cotton?

The aluminum foil is used to provide an extra layer of protection and ensure proper sterilization by preventing contamination.

How long and at what temperature are the tools sterilized in the autoclave?

The tools are sterilized in the autoclave at 121°C for 15 minutes.

What are the two types of media that are prepared and sterilized in the experiment?

The two types of media prepared are Nutrient Agar and Nutrient Broth.

How is the media prepared for sterilization in the experiment?

The required amount of Nutrient Agar and Nutrient Broth powder is weighed, added to Erlenmeyer flasks, and mixed with 300 mL of distilled water (aquades).

What happens after the media is mixed and heated on the hot plate?

Once the media becomes clear after heating, it is transferred from the hot plate, covered with cotton and aluminum foil, and labeled with the date and media name before being sterilized in the autoclave.