Fundamentals of Working Safely in a Biological Safety Cabinet (BSC): Preparing for Work in a BSC

Summary

This video provides a detailed step-by-step guide for preparing and using a Biological Safety Cabinet (BSC) in a laboratory setting. Key steps include gathering necessary materials, confirming BSC certification and safety features, ensuring proper airflow, and performing routine decontamination. Special attention is given to disinfectant use, proper cleaning techniques, and safety protocols. The importance of regular checks, such as pressure gauge monitoring and inward airflow verification, is highlighted. Proper posture and workstation setup are emphasized to ensure safety and efficiency. Following these guidelines helps maintain a sterile environment and minimize contamination risks.

Takeaways

• 😀 Schedule uninterrupted work time and gather all necessary materials (e.g., absorbent pads, biohazard waste container, pipettes, etc.) before using the BSC.
• 😀 Always confirm the UV light is turned off before starting work in the BSC.
• 😀 Ensure that the BSC certification is up to date and check the expiration date.
• 😀 Turn on both the fluorescent light and blower motor before starting any work in the BSC.
• 😀 Adjust the sash to the correct height as indicated on the BSC's external wall to avoid triggering the sash alarm.
• 😀 Ensure the drain valve is closed by checking that its handle is perpendicular to the valve body.
• 😀 Allow the BSC to run for at least 4 minutes to clear particulates from the air inside.
• 😀 Check the pressure differential gauge (magnehelic gauge) for any significant changes. A zero reading indicates a potential problem.
• 😀 Verify inward airflow using either a smoke generator or a tissue to ensure proper cabinet airflow.
• 😀 Clean all BSC surfaces with an appropriate disinfectant and disposable towels, avoiding spraying disinfectant inside to prevent damage.
• 😀 If bleach was used, wipe the BSC surfaces with water or 70% ethanol to remove bleach residue and prevent corrosion.

Q & A

Why is it important to schedule uninterrupted work time before starting in a BSC?

Scheduling uninterrupted work time ensures that you can complete the tasks without distractions, maintaining focus on safety and protocol during the work process in a BSC.

What materials should be gathered before starting work in a BSC?

You should gather absorbent pads, a biohazard waste container, pipettes, pipette tips, microfuge tubes, a vortex, a small sharps container, paper towels, and a squirt bottle with disinfectant, among other required materials.

What is the significance of checking the expiration date on the BSC certification?

Checking the expiration date ensures that the BSC is up-to-date and properly certified to operate safely. BSCs must be certified when installed, moved, and at least once annually.

What should be done if the UV light is on before starting work in the BSC?

You should confirm that the UV light is turned off, as UV light should not be on during normal BSC operations while working with biological materials.

Why is it important to check the pressure differential gauge (magnehelic gauge) on the BSC?

The pressure differential gauge monitors the filter's performance. A sudden drop in pressure indicates a possible block or breach in the filter, which can compromise the safety and airflow within the BSC.

How can you verify inward airflow in a BSC?

Inward airflow can be verified by using a smoke generator or by taping a tissue to the bottom of the sash. The tissue should be drawn into the cabinet, indicating proper airflow.

Why should the BSC be run for at least 4 minutes before use?

Running the BSC for at least 4 minutes allows the cabinet to purge the air of particulates, ensuring the workspace is clean and safe before starting any procedures.

What is the recommended method for decontaminating the inside of the BSC?

The inside of the BSC should be decontaminated using an appropriate liquid disinfectant that is effective against the infectious agents you're working with. The surfaces should be cleaned with a moistened towel to ensure proper contact time for disinfection.

Why should disinfectant not be sprayed inside the BSC?

Spraying disinfectant can create a mist that may be drawn into the grilles or HEPA filter, causing damage to the BSC's components and affecting the air quality inside the cabinet.

What should be done if bleach is used as a disinfectant in the BSC?

If bleach is used, the surfaces should be wiped again with water or 70% ethanol to remove any bleach residue, as bleach is corrosive and can damage the BSC’s stainless steel surface.