Prosedur pengujian toksisitas dengan metode BSLT (Brine Shrimp Lethality Test)

Summary

This video script outlines a toxicity test using the BSLT method with Artemia Salina larvae. The process involves hatching the larvae, preparing test solutions with varying concentrations, and observing their responses. Artificial seawater is mixed with extracts, and the larvae are exposed to different concentrations of the substance, including a negative control. After 24 hours under controlled lighting, the number of dead larvae is counted and compared to the control group. The procedure emphasizes the ease and speed of the method, along with its reliability at a 5% confidence level, and the results are analyzed across three replications.

Takeaways

• 😀 The BSlt method is used for toxicity testing, utilizing Artemia salina larvae as bioindicators.
• 😀 This method is quick, easy, and provides reliable results with a confidence level of 95%.
• 😀 The preparation involves weighing 9.5 grams of artificial seawater and dissolving it in 300 ml of distilled water.
• 😀 After incubation, the Artemia larvae are placed in artificial seawater and left for 48 hours under a lamp.
• 😀 The test solution is prepared by dissolving 10 mg of extract in 50 µL DMSO, then diluted with artificial seawater.
• 😀 Negative control consists of artificial seawater with DMSO, without the extract.
• 😀 A serial dilution is made, starting with a 2000 µg/ml solution and progressively reducing concentrations to 31.25 µg/ml.
• 😀 Each concentration is tested in triplicate to ensure reliability.
• 😀 Toxicity is tested by adding 10 larvae to each well containing different concentrations, including the control.
• 😀 After 24 hours, the larvae are observed, and the number of dead larvae is counted to evaluate the toxicity of the extract.

Q & A

What is the purpose of the BS-TL method in toxicity testing?

The BS-TL method is used for toxicity testing of compounds, utilizing other nuclear elements as bioindicators.

What are the advantages of using the BS-TL method?

The BS-TL method is known for its simplicity, speed, and a high confidence level, reaching 95%.

What is the first step in preparing for the toxicity test?

The first step involves hatching Artemia Salina larvae, where 9.5 grams of artificial seawater is dissolved in 300 mL of aquades, and then placed in an incubator for 48 hours.

How is the test solution prepared for the BS-TL method?

The test solution is prepared by dissolving 10 mg of extract in 50 µL of DMSO, then adding artificial seawater to make up a total of 5 mL.

What is the purpose of preparing different concentrations of the test solution?

Different concentrations are prepared to assess the toxicity of the compound at varying levels, ranging from 1500 to 31.25 µg/mL.

How are the toxicity test solutions organized on the plates?

The solutions are added to a 6-column plate in different concentrations, starting from 2000 µg/mL and then diluted through successive columns to create concentrations of 1500, 2550, 125, 62.5, and 31.25 µg/mL.

What does the control group consist of in this experiment?

The control group consists of artificial seawater and DMSO, without any test extract, to compare the results with the treated samples.

How are the larvae introduced to the testing plates?

10 larvae are placed in each well of the test plate that contains the respective concentrations of the test solution, as well as the control group.

What conditions are the larvae exposed to during the experiment?

The larvae are kept under a light source for observation and are left undisturbed for 24 hours.

How is the toxicity of the compound assessed in this experiment?

The toxicity is assessed by counting the number of dead larvae after 24 hours and comparing the results with the control group.