Muestreo de Superficies
Summary
TLDRThis video script outlines the process of surface sampling for microbial counts, specifically targeting 'Staphylococcus aureus'. It emphasizes the importance of using a template for consistent size to compare microbiological loads in a defined area. The script details the preparation of materials such as gloves, a sponge with a sterile bag, swabs, and a chain of custody form. It also describes the steps for sampling, including wearing protective gear, labeling the sterile bag, and using a sponge to collect samples from both smooth and irregular surfaces. The process involves careful handling, ensuring proper labeling, and storage in a pre-chilled container. Additionally, it covers hand sampling techniques using a swab moistened with neutralizing broth, ensuring thorough coverage of the entire hand.
Takeaways
- 🔬 The script discusses surface sampling for counting indicators such as 'Staphylococcus aureus'.
- 📏 The importance of using a template for surface sampling to compare microbiological loads in a defined area is highlighted.
- 📝 It is necessary to inform the laboratory of the template size for consistent sampling and comparison.
- 🧤 Materials required for sampling include gloves, a sponge with a sterile bag, a swab, a disinfectant solution, a template, a chain of custody form, and a thermal container with cold packs.
- 👷♂️ Personal protective equipment like a face shield and mask are essential for avoiding sample contamination.
- 📝 Labeling the sterile bag with sample information using a permanent marker is a crucial step.
- 🧽 For smooth surfaces, a template is placed and a sponge is used to rub the area to collect samples.
- 🔄 Both sides of the sponge are used to ensure complete coverage of the sampling area.
- 🧊 After sampling, the sponge is placed in a sterile bag, labeled, and stored in a pre-chilled thermal container.
- 🦠 When searching for pathogens on smooth surfaces, a template is not necessary, and the goal is to find any bacterial pathogen present.
- 🌡 For irregular surfaces, a swab with neutralizing solution is recommended to reach nooks and crannies.
- 👐 Hand sampling involves labeling with the person's name or employee number and using a swab moistened with neutralizing solution to cover the entire palm, between fingers, and under the nails.
Q & A
What is the purpose of using a template when sampling surfaces for microbiological counts?
-The purpose of using a template is to compare the microbiological load in a defined area, ensuring consistency in the size of the area sampled for accurate comparisons.
Why is it important to inform the laboratory about the size of the template used for sampling?
-Informing the laboratory about the template size allows them to include this information in the report, which is crucial for maintaining a record of samples and comparing results over time.
What materials are necessary for surface sampling as described in the script?
-The necessary materials include gloves, a swab, a sterile sponge with its sterile bag, a disinfectant solution, a template, a chain of custody or log, a pre-disinfected container with refrigerants, and hand sanitizer.
How should one prepare before taking a surface sample to avoid contamination?
-One should wear gloves, a mask, and put on a sterile gown. After washing hands and applying hand sanitizer, they should put on a new pair of gloves before handling the sterile sponge.
What is the correct procedure for sampling a smooth surface like a table?
-Select the area to be sampled, place the template on the surface, and carefully move it around to avoid contamination. Then, press the sponge against the surface within the area defined by the template to collect the sample.
How should the sponge be handled after collecting the sample from a smooth surface?
-The sponge should be placed in its sterile bag, ensuring it is properly labeled, and then stored in a pre-chilled container with refrigerants.
Why is a template not necessary when looking for pathogens on a surface?
-A template is not necessary because the goal is to find the presence or absence of pathogens, and any bacteria present on the surface is of interest, not just those within a defined area.
What is the recommended tool for sampling irregular surfaces?
-The recommended tool for irregular surfaces is a swab with neutralizing broth, which can reach small and hard-to-access areas, such as crevices.
How should hands be sampled for microbiological analysis?
-Hands should be sampled by breaking the seal of the swab to ensure the broth is wet, then rubbing the entire palm, between the fingers, and under the nails with the swab, ensuring full coverage.
What should be done after collecting a hand sample to ensure proper handling?
-After collecting the hand sample, the swab should be placed back into its container, sealed, and properly labeled before being stored.
Outlines
🔬 Surface Sampling for Microbiological Analysis
This paragraph describes the process of conducting surface sampling for microbiological counts, specifically for 'Staphylococcus aureus'. It emphasizes the importance of using a template to compare microbial loads within a defined area, regardless of its size. The script details the necessary materials, including gloves, a sponge with a sterile bag, a disinfectant swab, a transport chain or log, and a pre-chilled container with refrigerants. It also outlines the steps to avoid contamination, such as wearing a mask and gloves, labeling the sterile bag with sample information using a permanent marker, and applying alcohol after handwashing. The sampling technique involves rubbing a sponge within the area defined by the template and ensuring both sides of the sponge are used to cover the entire surface. The sponge is then placed in a sterile bag, properly labeled, and stored in a pre-chilled container. The paragraph also discusses the use of a swab for irregular surfaces and the need for a neutralizing solution.
👐 Hand Sampling Technique for Microbiological Assessment
The second paragraph focuses on the hand sampling technique used to assess the presence of microorganisms on human hands. It begins by detailing the labeling process with the person's name or employee number and the purpose of the sampling. The procedure involves breaking the seal of the swab to ensure the neutralizing solution is moist, and then rubbing the swab across the entire palm, between the fingers, and under the nails to ensure thorough coverage. The swab is then reinserted into its container and sealed. The paragraph highlights the importance of proper labeling and storage of the samples to maintain the integrity of the microbiological assessment.
Mindmap
Keywords
💡Surface Sampling
💡Template
💡Microbiological Load
💡Sterile Sponge
💡Sterile Bag
💡Thermometer Container
💡Hand Hygiene
💡Indicator
💡Irregular Surfaces
💡Swab
💡Chain of Custody
Highlights
Demonstration of surface sampling for counting indicators such as 'Staphylococcus aureus'.
Use of a template for comparing microbiological load in a defined area.
Importance of consistent template size for accurate comparison of samples.
Indicating template size to the laboratory for report inclusion.
Preparation of materials including gloves, swabs, and sterile sponge.
Utilization of a 'you swap' with letting broth for hygiene.
Use of an external template, chain of custody, and a pre-disinfected thermal container.
Hand hygiene with alcohol before starting the sampling process.
Wearing a mask and gloves to avoid sample contamination.
Labeling the sterile bag with necessary sample information using a permanent marker.
Selection of area for sampling on smooth surfaces like a table.
Use of both sides of the sponge to collect microbiological samples.
Placing the sponge in a sterile bag and ensuring it is properly labeled.
Storing the sample in a pre-cooled thermal container with refrigerants.
Sampling for pathogens without the need for a template.
Use of a swab to collect samples from irregular surfaces.
Recommended use of a swab with neutralizing broth for hard-to-reach areas.
Hand sampling procedure using labeled swabs with individual or employee identification.
Ensuring the swab is moistened with neutralizing broth before hand sampling.
Thorough rubbing with the swab to cover the entire palm, between fingers, and under the nails.
Proper sealing and labeling of the swab after hand sampling.
Transcripts
muestreo de superficies
primero demostraremos cómo hacer un
muestreo de superficies para recuentos
de indicadores o 'staphylococcus aureus'
en este caso si debemos utilizar una
plantilla porque queremos comparar la
carga microbiológica en un área definida
no importa el tamaño de la plantilla
siempre y cuando hicimos el mismo tamaño
si queremos tener un historial de los
muestreos y poder comparar resultados
se debe indicar al laboratorio el tamaño
de la plantilla para incluir esta
información en el informe primero
debemos tener listos nuestros materiales
guantes
a través y mascarilla esponja con su
bolsa estéril hisopo nosotros utilizamos
you swap con caldo letting de higiene
plantilla externa cadena de custodia o
registro y un contenedor térmico en
compass refrigerantes previamente
desinfectados tener alcohol a la mano
para iniciar debemos primero colocarnos
la redecilla y mascarilla para evitar
contaminar la muestra
las manos y procedemos a rotular la
bolsa estéril con la información
necesaria de la muestra debemos usar un
marcador permanente
después de lavarnos las manos aplicamos
alcohol y nos colocamos un par de
guantes nuevos
[Música]
sacamos la esponja en el empaque con
mucho cuidado
ah
si es una superficie lisa como una mesa
escogemos el área muestrear y colocamos
la plantilla esté dentro moviéndola
cuidadosamente de su empaque procedemos
a frotar dentro del área delimitada por
la plantilla presionando la esponja para
sustraer cualquier adherencia de la
superficie utilizamos ambos lados de la
esponja y nos aseguramos de cubrir cada
espacio del aperitivo
[Música]
luego colocó la esponja en su bolsa
estéril y me aseguró que está
adecuadamente titulada enlace yo
procedo a guardarle mi termo compasión
friger antes previamente de simple
tiramos
muestreo de superficies para patógenos
nuevamente nos colocamos un par de
guantes nuevos
y sacamos la espuma de su empaque
[Música]
[Música]
si es una superficie lisa como una mesa
procedemos a afrontar un área amplia
presionando la esponja para sustraer
cualquier adherencia de la superficie no
es necesario una plantilla cuando
estamos buscando patógenos ya que
estamos buscando ausencia o presencia de
patógenos así que lo que queremos es
encontrar cualquier patógeno bacteria
que pueda estar elisa
luego colocó la esponja en su bolsa
anterior y nos aseguramos que esté
adecuadamente rotulado
ah
[Música]
porque está sellada y la introducimos en
interno con más refrigerantes
previamente
[Música]
superficies irregulares y manos cuando
vamos a muestrear una superficie
irregular el implemento más recomendado
es un hisopo con cartón neutralizante el
cual puede introducirse en nichos y
lugares pequeños difíciles de alcanzar
nosotros usamos que usual con caldo
letting de y
aunque vamos a hacer conteo de
microorganismo muchas veces en esta
superficie
no es posible aplicar una plantilla por
lo que los resultados se reportan por
superficie
en este caso una espátula luego de
lavarte las manos
aplicamos
y unos guantes
rod unamos el sombrero
[Música]
y procedemos a romper el sello del medio
para asegurarnos que el sopor esté
húmedo
procedemos a tomar la muestra frotamos
con el hisopo aplicando presión al
implemento
y procuramos cubrir toda el área
al terminar introducimos ahí su cuenta y
recipiente y los sellamos
y luego procedemos a asegurar que esté
bien rotulado y con cuerda en el área
que se acaba de mostrar lo introducimos
cuando vamos a tomar muestra de manos
utilizamos nuevamente
rotulados con el nombre de la persona o
el número de empleado y pon
y para qué se va a tomar
procedemos a romper el 612 para
asegurarnos que el eso fue éste el
húmedo con caldo neutralizante
procedemos a frotar con el hisopo y nos
aseguramos de cubrir la palma entera
la mano entre los dedos y hasta debajo
del esquí
[Música]
una vez hemos cubierto con el listón y
introducimos el hisopo nuevamente dentro
y nos aseguramos que este miembro pudo
lo colocamos en internet
[Música]
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