Pelatihan Analisis Instrumen Kimia Pangan Olahan dan Air: Penetapan Kadar Pb dalam Yogurt secara AAS

Summary

This video script offers an in-depth look at a chemical analysis training at the Balai Besar POM in Semarang. It walks through the process of verifying PKPB in yogurt, emphasizing safety protocols like wearing complete lab gear. The procedure includes the preparation of chemical solutions, sample testing, and calibration. It highlights the importance of accuracy and precision, ensuring high-quality results. Additionally, it encourages adherence to health protocols and provides contact details for further inquiries. The message stresses transparency and the rejection of corruption within the institution.

Takeaways

• 😀 The BPOM office in Semarang emphasizes cleanliness and commitment to serving the public through a strong bureaucratic system.
• 😀 Before entering the laboratory, personnel are required to wear complete personal protective equipment (PPE), including lab coats, gloves, masks, and shoes.
• 😀 The laboratory procedure for verifying the PKPB in yoghurt involves a series of chemical treatments and strict handling protocols.
• 😀 The materials used include H2O2 (30%), A37V (0.365%), and Aqua DM, with samples soaking overnight before treatment.
• 😀 After soaking, the samples undergo rinsing with Aqua DM three times before being dried and treated with nitric acid (HNO3 60%).
• 😀 The samples are then processed in a microwave and allowed to cool to room temperature for further analysis.
• 😀 A matrix modifier solution is prepared using phosphoric acid and is diluted with aquades for use in subsequent steps.
• 😀 Calibration standards are prepared with PB6 (6 mg/mL) and work standards at 60 Nanu (nanogram per mL).
• 😀 The readings for AAS (Atomic Absorption Spectroscopy) are set at a wavelength of 287 nm to ensure accurate results.
• 😀 A calibration curve is generated, achieving a high R-squared value of 0.996, ensuring reliable analysis of the sample results.
• 😀 The final steps involve measuring the blank, precision samples, and accuracy samples, followed by calculating recovery and preparing the final report.

Q & A

What is the main objective of the laboratory procedure in the script?

The main objective of the laboratory procedure is to verify and analyze the content of yogurt, focusing on a chemical analysis process using specific reagents and equipment.

What Personal Protective Equipment (PPE) is required before entering the laboratory?

Before entering the laboratory, it is essential to wear a lab coat, gloves, a mask, and shoes for safety and protection.

Which chemicals are used in the analysis process of yogurt in the lab?

The chemicals used include Hydrogen Peroxide (H2O2) 30%, Nitric Acid (HNO3) 60%, Hydrochloric Acid (HCl) 37%, and Aqua DM (distilled water).

What is the procedure for preparing the samples before analysis?

Samples are soaked in a 10% Aqua DM solution overnight. After 24 hours, they are rinsed three times with Aqua DM, dried, and treated with HNO3 before being microwaved for further processing.

How is the matrix modifier solution prepared?

The matrix modifier solution is prepared by adding 10 mL of Aqua DM to a 25 mL flask, then adding 300 µL of 60% Phosphoric Acid and diluting it with Aqua DM to the mark.

What is the purpose of the calibration curve in the analysis?

The calibration curve is used to obtain an accurate measurement of the sample's concentration by creating a relationship between known standard concentrations and the instrument's readings.

What steps are involved in preparing the sample for Atomic Absorption Spectroscopy (AAS)?

To prepare the sample for AAS, it is first transferred into a 25 mL flask, followed by adding 0.1 mL of the Pb standard. Then, the solution is diluted with Aqua DM to the mark before injection into the AAS.

What is the wavelength used for the Atomic Absorption Spectroscopy (AAS) analysis?

The AAS analysis is performed at a wavelength of 287.31 nm for the detection of lead (Pb).

How is the accuracy of the procedure confirmed during the analysis?

The accuracy is confirmed by analyzing the calibration curve, obtaining a high R-value (0.996), and calculating the recovery rate after analyzing the blank, sample precision, and accuracy.

What should one do before consuming or purchasing any medicines or foods according to the protocol?

Before consuming or purchasing any medicines or foods, it is important to check the packaging, label, expiration date, and certification number to ensure safety and quality.