I Genetically Engineered *MYSELF* to Fix Lactose Intolerance

Summary

This video documents a personal journey to combat lactose intolerance through DIY gene therapy. The creator, once severely affected by lactose, explains the science behind lactose digestion and the enzyme lactase. They detail the process of using an adeno-associated virus (AAV) to deliver a lactase gene to the intestinal tract, successfully restoring their lactose tolerance. The video provides a transparent look into the lab work, the challenges faced, and the successful consumption of dairy products post-treatment, promising a future of enjoying pizza without fear.

Takeaways

• 😷 The speaker became lactose intolerant at 15, leading to severe illness from consuming lactose.
• 🌐 Up to 65% of the world's population may lose the ability to digest lactose, causing various symptoms.
• 🍽️ Lactose is prevalent in many foods, often as a filler or sweetener, due to the dairy industry's by-products.
• 🔬 The speaker's personal struggle with lactose intolerance inspired a career in biology, aiming to restore their own lactose tolerance.
• 🧬 Lactose intolerance occurs when the body stops producing lactase, the enzyme that breaks down lactose into glucose and galactose.
• 🐁 A study mentioned used a virus to deliver a working copy of the lactase gene to lactose-intolerant rats, successfully restoring their lactose digestion.
• 🌟 Adeno-associated viruses (AAVs) are preferred for gene therapy due to their safety and reliability.
• 🧪 The process of creating a virus for gene therapy involves using plasmids and cells in a lab, with careful handling to ensure sterility.
• 💊 The speaker's DIY gene therapy project aimed to create a treatment in pill form to restore lactose tolerance.
• 🍕 After testing the treatment, the speaker was able to consume dairy products without symptoms, marking a successful initial result.

Q & A

What is the main issue the speaker discusses in the script?

The speaker discusses their personal struggle with lactose intolerance and their journey to develop a DIY gene therapy to restore their lactose tolerance.

What is the percentage of the human population that may become lactose intolerant at some point?

Up to 65% of the human population may become lactose intolerant at some point.

What is lactose and why is it problematic for lactose intolerant individuals?

Lactose is a disaccharide composed of glucose and galactose subunits. It is problematic for lactose intolerant individuals because they lack the lactase enzyme to break it down, leading to gastrointestinal issues when consuming lactose-containing foods.

What is the role of the lactase enzyme in digestion?

The lactase enzyme, also known as beta-galactosidase, breaks down lactose into its subunits, glucose and galactose, which can then be absorbed by the body for energy.

Why is the adeno-associated virus (AAV) considered the gold standard for gene therapy?

The AAV is considered the gold standard for gene therapy due to its reliability, long history of safety, and its ability to merge with human DNA in very safe and well-known locations.

What is the purpose of the ITRs in the context of the virus production?

ITRs, or inverted terminal repeats, are DNA sequences that signal the virus to package the DNA sequence between them into its shell during virus particle formation.

What are plasmids and how are they used in this project?

Plasmids are small circles of DNA engineered by biologists to contain useful features. In this project, they are used to hold the DNA sequences for the virus shell proteins and the Lac Z gene, which is the target gene for restoring lactose tolerance.

How does the speaker plan to test the effectiveness of the gene therapy?

The speaker plans to test the effectiveness of the gene therapy by using a color-changing dye called X-Gal, which turns blue in the presence of the lactase enzyme, indicating that the cells are producing the enzyme.

What is the significance of the speaker's successful consumption of pizza after the treatment?

The successful consumption of pizza, which is high in lactose, without experiencing symptoms of lactose intolerance, signifies that the DIY gene therapy may have successfully restored the speaker's lactose tolerance.

What is the next step for the speaker in terms of this project?

The next step for the speaker is to continue testing and refining the treatment, monitoring its long-term effects, and potentially sharing updates on the project's progress.

Outlines

00:00

🥶 Lactose Intolerance Struggles and DIY Gene Therapy

The speaker shares their personal journey with lactose intolerance, which began at the age of 15, causing frequent illness. They explain how lactose, a sugar found in milk products, affects those who cannot produce lactase, the enzyme required to break it down. The lack of lactase results in symptoms like bloating and diarrhea when consuming dairy products. The speaker discusses the prevalence of lactose in various food items due to its use as a filler and sweetener. They also delve into their motivation to study biology to find a solution to their lactose intolerance, aiming to restore their ability to consume dairy without repercussions. The narrative culminates in their pursuit of DIY gene therapy as a means to treat their condition, with a focus on transparency and education about the process.

05:01

🧬 Understanding Lactose and Gene Therapy

This section delves into the science behind lactose intolerance and the proposed gene therapy treatment. Lactose is described as a disaccharide composed of glucose and galactose, which is broken down by the lactase enzyme. When lactase is absent, lactose reaches the colon, causing gastrointestinal issues. The speaker outlines a method using a virus to deliver a functional lactase gene to the intestinal tract, as demonstrated in a study on rats. The video aims to demystify DIY gene therapy, emphasizing safety and clarity in the process. It also introduces adeno-associated virus (AAV) as a preferred vector for gene therapy due to its safety and efficiency. The process of creating the virus involves using specific DNA sequences and cellular machinery to package the therapeutic gene into the viral shell.

10:02

🔬 Lab Work: Cultivating Cells and Preparing for Gene Therapy

The speaker describes the laboratory procedures involved in preparing for gene therapy. This includes the cultivation of human embryonic kidney (HEK) and Chinese hamster ovary (CHO) cells, which are used for producing viral particles. The cells are grown in a sterile environment using specialized media and equipment. The process involves the use of lip effect amine to facilitate the transfer of DNA into the cells, which is a critical step in the production of the viral vector. The speaker also details the steps for transducing the cells with the viral particles carrying the lactase gene, including the use of a color-changing dye (X-gal) to confirm successful gene expression. The video provides a behind-the-scenes look at the lab work and the challenges faced during the experiment.

15:04

🍕 Testing the DIY Gene Therapy: A Personal Trial

In the final paragraph, the speaker recounts their personal experience testing the DIY gene therapy. After a period of allowing the virus to deliver the lactase gene, the speaker consumes dairy products to test the effectiveness of the treatment. They report a successful outcome, with no adverse reactions to lactose after taking the gene therapy. The speaker expresses their excitement and relief at being able to enjoy dairy products without the previous symptoms of intolerance. They acknowledge that the project is ongoing and that further testing and refinement are necessary. The video concludes with a call to action for viewers to support future videos and a note of gratitude to patrons who have contributed to the project.

Keywords

💡Lactose intolerance

Lactose intolerance is a condition where an individual's body lacks the enzyme lactase, which is necessary for breaking down lactose, a sugar found in milk and dairy products. This leads to gastrointestinal symptoms like bloating, gas, and diarrhea after consuming lactose-containing foods. In the video, the creator discusses their personal experience with lactose intolerance, which has significantly impacted their diet and led to their interest in biology and gene therapy.

💡Lactase

Lactase, also known as beta-galactosidase, is the enzyme responsible for breaking down lactose into glucose and galactose, which can then be absorbed by the body. The video script describes how a lack of lactase production in the small intestine leads to lactose intolerance. The creator's project aims to induce lactase production in the body to treat lactose intolerance.

💡Adenoviral-associated virus (AAV)

Adenoviral-associated virus (AAV) is a type of virus used in gene therapy due to its safety and reliability. It is often used as a vector to deliver functional copies of genes into target cells. In the script, the creator mentions using an AAV to deliver a working copy of the lactase gene to the intestinal tract, which is a key part of their DIY gene therapy project.

💡Gene therapy

Gene therapy involves introducing, altering, or manipulating genes to treat or prevent disease. The video's central theme revolves around DIY gene therapy, where the creator attempts to develop a treatment for lactose intolerance by introducing the lactase gene into their body to restore their ability to digest lactose.

💡Lactose

Lactose is a disaccharide sugar found in milk and dairy products, composed of glucose and galactose subunits. The video explains that lactose intolerance occurs when the body cannot break down lactose due to a lack of lactase enzyme, leading to symptoms when lactose reaches the colon and is fermented by bacteria.

💡Lac Z gene

The Lac Z gene, derived from E. coli, encodes the lactase enzyme. In the video, the creator uses this gene in their gene therapy project to enable the production of lactase in the body. The script describes how this gene is packaged into a virus for delivery into cells.

💡X-Gal

X-Gal, or 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside, is a color-changing dye used to detect the presence of beta-galactosidase (lactase) activity. In the script, the creator uses X-Gal to test whether the Lac Z gene has been successfully expressed in cells, as the presence of lactase will cause the dye to turn blue.

💡Lipid-mediated transfection

Lipid-mediated transfection is a technique used to introduce DNA into cells using lipids, which form a protective bubble around the DNA, allowing it to cross the cell membrane. In the video, the creator uses lipofectamine, a lipid-based reagent, to facilitate the transfer of the Lac Z gene into cells to produce the virus particles.

💡Plasmids

Plasmids are circular DNA molecules used as vectors in genetic engineering to carry a DNA sequence of interest into a host cell. The video script describes how the creator uses plasmids to carry the necessary DNA sequences for the virus shell and the Lac Z gene into cells to produce the gene therapy virus.

💡Inverted terminal repeats (ITRs)

Inverted terminal repeats are specific DNA sequences found at both ends of a virus genome that play a crucial role in the replication and packaging of the viral genome. In the context of the video, ITRs are used to mark the DNA sequence that should be packaged into the virus particle, ensuring that the Lac Z gene is included in the virus for gene therapy.

💡Biosafety

Biosafety refers to the practices and measures taken to prevent harm from biological materials and processes. The video script mentions the use of a biosafety hood to maintain sterility during the process of handling cells and DNA, which is crucial to prevent contamination and ensure the safety of the gene therapy experiment.

Highlights

Individual developed lactose intolerance at 15, experiencing severe symptoms from consuming lactose.

Up to 65% of the human population may lose the ability to digest lactose, leading to various symptoms.

Lactose is a disaccharide found in many food products, including those using lactose as a filler or sweetener.

Lactase enzyme is crucial for breaking down lactose; without it, lactose intolerance symptoms occur.

Individual pursued biology to find a solution to restore lactose tolerance, aiming for personal dietary freedom.

DIY gene therapy is used to develop a treatment to restore lactose tolerance, using adeno-associated virus (AAV).

AAV is a safe and reliable vector for gene therapy, commonly found in humans without causing illness.

Plasmids are used to carry the DNA sequences for virus shell proteins and the Lac Z gene for lactase production.

Lipid-based transfection agents, like Lipofectamine, are used to deliver DNA into cells for virus production.

Two types of cells, HEK and CHO, are used for virus production, with different growth characteristics.

X-Gal assay is used to test for the presence of the Lac Z gene, indicating successful gene delivery and expression.

Virus harvesting involves pelleting cellular debris and precipitating the virus with ammonium sulfate.

Initial testing of the DIY gene therapy involved consuming lactose-rich food to test for restored tolerance.

Individual successfully consumed pizza and milk without symptoms, indicating potential success of the treatment.

The project is ongoing, with plans for further testing and refinement to ensure long-term effectiveness.

Individual encourages viewers to subscribe for updates on the project's progress and success.

Transcripts

[Music]

and in media and all these my stomach

release the virus into the virus and go

into my intestinal lining

when I was 15 I started getting sick all

the time at first it was pretty

infrequent but I remember the day that

it started I didn't know at the time

that I'd suddenly become

lactose-intolerant so all the food I was

eating was hurting me

laugh it off but in my case it wasn't

just a bit of gas I was getting

violently ill if I ate even a minut

amount of lactose and symptoms would

start in less than an hour and I'm not

alone this happens to up to 65% of the

human population at some point your

ability to eat milk just vanishes and

you start getting sick symptoms severity

varies but the extreme cases like mine

can really get in your way

most people don't actually think about

how much lactose is in the food we eat

as a society we actually produce so much

milk for you some cheese making that

it's literally a waste product so all

the components are separated out and

used and resold for a variety of

purposes the sugars specifically lactose

are refined and used as filler in flavor

in an enormous amount of products most

pharmaceutical manufacturers uses to

bulk up drugs used to make pills so the

tiny amount of drug that they need to

carry can be pressed into a

comparatively large pill whey is used as

a sweetener butter is in everything as

his cheese and cream I basically had to

build a whole skill set around making

sure food was safe to eat Irie taught

myself to cook and learn how to bake so

that I didn't have to miss out on too

much good food but all of this is also

the reason I went into biology from the

day I decided on the university program

I was going to apply for I've said the

same thing

one day I'll fix this when I was asked

if I was going to biology to end world

hunger or some other grand gesture my

response has always been now dude I just

want to eat pizza again and not need to

check every label on everything and I

can happily say that that day is finally

here after six years I finally have

access to the tools and know-how to

build a permanent treatment that should

restore my lactose tolerance and fix

this once and for all now I know the

idea of the DIY gene therapy can seem

intimidating or scary so let me set the

record straight

while some users make the application of

DIY gene therapy looks sloppy and

sketchy I'm hoping to change that with

this video I'm going to be as

transparent and make sure everything is

as clear as possible so you know exactly

what I'm doing how the thing works and

why it's not scary also this project is

still in the process of being developed

this video is just the first

demonstration of the process I'll be

continuing to refine it over the next

little while also

is going to be a long ish video so if

you'd like to skip to the testing and

results go to the time you see on screen

before we get into the lab and get

working let's go over how this is going

to work first we need to understand both

lactose and the enzyme that breaks it

down lactose is a disaccharide or simple

sugar that has two subunits one glucose

and one galactose subunit lactase also

known as beta galactosidase the enzyme

that breaks it down splits the two

subunits apart and then the individual

pieces can actually be absorbed by your

body and used for energy if you don't

produce the lactase enzyme the sugar

will make it all the way through your

small intestine and into your colon your

colon is full of a lot more bacteria

especially gas producing bacteria and so

they feed on the influx of extra

available food because the lactose

wasn't broken down and absorbed also

lactose attracts and holds on to water

so a lot less water is absorbed in your

colon that is supposed to be a DIN

irritation you've got all the classic

symptoms of lactose intolerance so what

do we do about it

well we need to make my small intestine

produce the lactase enzyme again

specifically in what's called the brush

border the brush border is a layer of

cells covered in tiny hairlike

protrusions these increase the surface

area of the small intestine

significantly these protrusions are also

packed full of enzymes that interact

with the material passing through and

helps break them down we could use the

human gene for lactase but it's actually

standard practice to use lactase enzyme

from E coli called Lac z lac c is used

all throughout biology and in

combination with a color changing dye

can be used as a marker to indicate if

cells are doing what they're supposed to

be doing when I was looking into this I

found a paper that uses a special virus

to deliver a working copy of lac z to

the intestinal tract of rats a week

after the researchers applied the virus

they challenged two sets of rats that

had previously been completely lactose

intolerant with a lactose only diet the

rats that have been given the virus

maintained their weight and their blood

glucose levels went up after they ate

the lactose indicating that they had

started to produce the lactase enzyme

and was able to use it for calories the

virus they used is called an AAV or

adeno-associated virus this virus is the

gold standard for gene therapy because

its reliability and long history of

safety the wild virus can be found in

almost everyone and in most tissue

samples that have ever been tested so

it's extremely common but it doesn't

cause any sort of illness it just kind

of hangs out it can't even replicate on

its own for it to spread you need to be

infected with its second actual

adenovirus hence why it's called an

Deno associated virus it just sort of

hitches a ride also it has the useful

property that the DNA that gets packaged

inside of it often merges with your DNA

but it does so only in a couple of very

well-known locations that have been

shown to be very very safe so how do you

make the virus and how do you choose

what DNA ends up on the inside well it

all works on a very clever system to

make a virus particle you need all of

the sequences of DNA that code for all

the proteins that make the shell of the

virus to be transcribed in the same cell

the various pieces will float around

until they bump into the rest of the

pieces and then they sort of snap

together to form the virus particle in

order for the virus to know which DNA is

owned and what to package into the shell

it uses two DNA sequences called ITRs or

inverted terminal repeats if you put

these on either side of some other DNA

sequence everything between the ITRs get

packaged normally this would be in the

virus packages DNA that contains all of

the information to make all of its own

pieces but what biologists did is break

their DNA into three separate pieces the

first to contain all of the sequences to

make all of the proteins but lack the

ITR sequences the last contains the ITRs

and whatever DNA we want a package by

the way these three pieces of DNA are

just called plasmids they're basically

just small circles of DNA that

biologists have engineered to have some

useful features I like to think of them

like CDs for a computer they hold a bit

of code that contain the instructions

for a piece of software when you run it

the program executes and something

happens in this case when the plasmid

makes it to the nucleus of a cell it

starts being translated into RNA and

then that gets translated into protein

let's take a closer look at the plasmids

to see what's actually going on on the

first two plasmids you can see that

proteins that make up the shell of the

virus and the rest of the proteins that

help it move and copy its DNA and

package everything you'll notice that

both have something called an Ori and a

gene that codes for ampicillin

resistance that's how you can put this

DNA into bacteria and use them as tiny

copying machines to make a literal

bucket worth of DNA to be used in future

experiments we'll be exploring that

process in a future video

the last plasmid is where all of the

important parts are here you can see the

ITR as I mentioned before this part is

called a promoter

it's a sequence that encourages certain

proteins to bind to that spot and start

transcribing the DNA

to RNA to get the process started then

right after it we have the Lac Z gene

that we want and finally something

called a poly a signal this is just a

part that's necessary for when the

protein is actually being made for both

the promoter and poly a signal there are

a ton of options to choose from but this

particular plasmid uses the CMV promoter

and an sv40 poly a signal the CMV

promoter is really good for when you

want lots of protein to be produced

wherever the DNA happens to be running

to actually make the virus particles you

take all three pieces of DNA and using a

special agent to help it enter cells you

apply it to a flask of mammalian cells

the cells will start producing the virus

particles and filling them with the DNA

and then they exit the cell all we need

to do is harvest the virus purify it

into a safe to consume form and package

it into a pill okay now that we know how

this works let's get to work

I'm here in my friend's genetics lab and

he's letting me use his equipment and

materials so I can do this project for

this we'll need a few things first is

the three pieces of DNA the two virus

plasmids come as a kit and I bought the

lac C plasmid separately we'll be using

two kinds of cells HEC or human

embryonic kidney and Cho or Chinese

hamster ovary heck are the standard for

making things like viruses and proteins

but Cho are equally good

HEC cells or at least the kind that we

use our suspension cells and like to

float around whereas Cho cells are

adherent and actually stick to the

culture flask to feed the cells and keep

them happy we'll need special media I

won't show you how to prepare the media

as well save that for a future video and

finally to get the DNA into the cells

we'll be using a special chemical called

lip effect amine first things first we

need to start some cells all of our work

will be taking place in a biosafety to

laminar flow hood this keeps everything

super sterile and prevents ourselves

from getting contaminated or other

viruses getting in also we use

antibiotics in the media to further

limit contamination specifically we use

a mixture of penicillin and streptomycin

before entering the hood to spray down

the surface with alcohol put on gloves

and then spray your arms up to the elbow

with the alcohol before bringing any

materials into the hood they must be

sterile and the container needs to be

sprayed with alcohol before we can use

the media we need to warm it up to body

temperature to keep the cells happy

we're using a water bath for this and

check the temperature until the media is

at least 32 degrees

but no warmer than 37 degrees Celsius

when it's a temp we can dry it off

sterilize the bottle and bring it into

the hood to move the media around we're

using special serological pipettes that

have the wide opening and come pre

sterilized and individually wrapped

we're also using an auto pipettor that

makes it easy to draw and release

liquids we'll be growing ourselves in a

special tissue culture flask that comes

pre sterilized in this case we'll be

using a small 25 centimeter flask that

holds a maximum of 5 milliliters of

liquid the first step is always to label

your flask with the cell type the

experiment and the date first let's look

at what we do for the cho cells we can

remove the lid of the flask and their

lid of the media placing the lids

facedown on the work surface then set up

the auto pipette or with a fresh pipette

and draw up four mils of media for cho

cells that means F 12 K media

supplemented with 10% fetal bovine serum

pick up the flask tilt it and dispense

the liquid it's good practice to limit

things touching as much as physically

possible then disposes the pipette and

then tilt the dish back and forth until

the media covers the bottom with the

flask prepared we can add ourselves I'm

using a regular P 1000 micropipette and

I'm adding the cells that we grew and

prepared in advance I'll show how we did

that in a future video for the HEC cells

we did basically the same thing but

because their suspension cells we just

grew them to the density we wanted in a

larger flask and then pulled off 5

milliliters worth of suspension and

transfer that to a new flask to be used

immediately the cho cells need more time

to grow though so those get transferred

to a co2 humidified incubator set at 37

degrees Celsius and 5% co2 since the HEC

cells are already ready we can go

through the transduction procedure for

this we'll need the lip effect amine

which comes in two parts the media for

the HEC cells and the three DNA

solutions to set this up we first put

500 microliters of HEC media into two

sterile DNA free EPI tubes we want five

micrograms of DNA total which works out

to 1.6 micrograms of each type of DNA or

6.8 microliters of each DNA solution add

that to one of the tubes and to the same

tube add 5 microliters of lip effect

amine plus reagent to the other tube we

add 5 microliters of regular lip effect

amine give those mix then mix the DNA

solution into the lip effect amine not

the other

way around allow that to incubate for

five minutes lip effect Amin is sort of

an oil that forms a bubble called lipids

ohms around the DNA that allows it to

get across the cell membrane it takes a

few minutes for the lipids ohms to form

hence the incubation time after the

incubation all that we have do is add

the solution to the flask of cells with

that done the flask can be transferred

to the incubator the reason we're using

two cell types is because I actually

tried this experiment twice the first

time was with the HEC cells but the

cells died because the live effect Amin

is kind of aggressive and can damage the

cells the second time the procedure was

the same but i use the cho cells which

because they're adhered to the dish have

the added benefit that 12 hours after

the transfection I can change the media

to fresh lip effect amine free media

this keeps the cells much healthier the

cho cells work so we let them sit and

produce as much virus as possible for

three whole days did you have to see if

this worked we'll be using a

color-changing guy called ex Cal ex

Cal's a dye molecule fused to a

galactose molecule so that if lactase is

present the two gets split the dye

becomes active and turns blue and we

know that the thing is working I made a

solution of two milligrams of X Cal in

five milliliters of DMSO this has the

benefit that DMSO will carry the X gal

into cells that we exposed to it also

DMSO is one of the only things that can

actually dissolve ex Cal because the cho

cells were adhered before we do the

tests we can collect all the media which

is where most of the virus is going to

be save this in a separate sterile

Falcon tube then we can test the cells

themselves first we need to make them

let go of the dish so that we can take a

sample and test them to do that we'll be

using two milliliters of pre-made

trypsin solution allow the trypsin to

sit in the dish until the solution

starts to get a bit cloudy this is

evidence that the cells have let go and

are floating around when this happens I

use a pipette to collect all of the

cells and transfer them to a falcon tube

these then get loaded into a centrifuge

and spun down at 5000 rpm for five

minutes two pellet them onto the bottom

so that we can remove the trypsin when

that's done we need to reach sterilize

everything and bring it back into the

hood so that we can take our sample I

removed the trypsin solution and

resuspended the cells in two milliliters

of media and then took a two hundred

microliters sample and transferred it to

a small tube I added an equal volume of

x-gal and then let everything sit for a

while to give it time for the reaction

to occur if any lactase is present after

the incubation I took everything to the

micro

scope so that we can see what actually

happened it was really hard to capture

with my camera but the cells were

definitely blue and with that I knew

that everything had worked and that we

can finally harvest the virus for this

very first round of testing we'll

actually be using a really

straightforward method to harvest our

virus first things first the media full

of virus were that we saved has to be

spun down at 2,000 rpm to pellet any

cellular debris the virus is super light

so it'll just stay floating around

collect the supernatant and leave any

salts behind to get the virus out of the

solution we can add ammonium sulfate

solution this is what's known as salting

out ideally you'd do this in two steps

to first remove the residual protein and

then pellet the virus but I'm just going

to be doing this all in one step I'm

adding an equal volume of 4 molar

ammonium sulfate solution this sudden

influx of salt makes the virus

precipitate out so that we can actually

collect it let that sit for an hour and

then spin it down at 4,000 rpm to

collect the product now I know this will

be contaminated with other proteins but

for this test I judged that to be an

acceptable risk based on all of the

possible protein contamination in the

future this will be refined much further

and more purification steps will be in

place to make sure the virus is truly

the only thing in here remove the

supernatant and discard it then Reda's

all've the viral pellet in 800

microliters of sterile phosphate

buffered saline and that's most of it

done all that's left is to package this

into a pill to do that and mix the

solution with a minimal amount of micro

crystalline cellulose and then put that

into gel caps using this handy gel cap

jig machine thing ideally I should have

freeze-dried the powder before packaging

but again for the initial test I decided

I didn't want to wait after taking the

pills I waited three days to give the

virus time to deliver the DNA and give

my cells time to start producing lactase

and then it was time to test it we

ordered the milk iasts cheesiest pizza

we could

try to be less scary here is the mind

killer is it good can you not tell now

I'll be honest this was maybe one of the

most stressful moments in recent memory

willingly eating something that would

normally make me extremely sick scared

the hell out of me

but I had ate several pieces and then

settled into a movie after an hour I

felt fine after a whole night's sleep I

still felt fine and after 48 hours I was

completely fine I couldn't believe it

but the therapy seemed to have actually

worked in two weeks as you can see got

more pizza I've enjoying just the most

copious amounts of lactose filled foods

for last two weeks it's been wonderful I

feel better than I have in eight years

at first when I was testing this I would

still get a little bit of gas but that's

completely gone away now I think it just

took a while for more protein to be

expressed and for my microbiome to

readjust to the radical change in diet

as I said this project is still in the

development stage and more testing will

need to be done I'll be taking notes to

see if the effect wears off after some

time and I'll be sure to keep you

updated if this project interested you

and you want to see more I'd really

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possible that's all for now and I'll see

you next time