Validasi Reagensia Antisera A dan B menggunakan Sel A, Sel B dan Sel O 5%

Summary

This video explains the process of validating antisera for blood group testing, specifically Anti-A and Anti-B. The procedure begins by preparing a 5% cell suspension from a verified blood donor and testing the antisera after opening a new package. The validation steps include using specific tools such as test tubes, a validation card, and centrifugation to observe agglutination reactions. The process helps determine the validity of antisera for daily serological tests. The video also covers the importance of using fresh cell suspensions and proper storage to ensure accurate results.

Takeaways

• 😀 Prepare a 5% cell suspension (A, B, and O cells) for validating antisera A and B.
• 😀 To validate antisera, use a known blood sample to prepare the cell suspension.
• 😀 Always check the expiration date and proper storage conditions (2-8°C) before using antisera.
• 😀 When validating antisera, it is essential to use fresh antisera from newly opened packages.
• 😀 Follow a systematic procedure for validating antisera, including specific steps for agglutination testing.
• 😀 Prepare 3 test tubes: one for anti-A, one for anti-B, and one for the O control.
• 😀 Apply 1 drop of the cell suspension and 2 drops of antisera for each test tube.
• 😀 After adding the antisera, homogenize the mixtures and centrifuge them at 2,000-3,000 RPM for about 15 seconds.
• 😀 Inspect the results for agglutination, which indicates a positive result (clumping of red blood cells).
• 😀 Record the results on a validation card, ensuring the test is performed correctly and within the prescribed timeframe.
• 😀 Ensure that the cell suspensions are used within 3 days to avoid hemolysis, and store the solution for up to 30 days using saline solution.

Q & A

What is the first step in validating antisera for blood groups A and B?

The first step in validating antisera for blood groups A and B is to prepare a 5% suspension of cells A, B, and O, which must be sourced from a person whose blood group is already confirmed.

How should antisera be validated?

Antisera should be validated by performing a test using agglutination. The antisera must be opened from a newly sealed package, and the validity is checked using cells A, B, and O at a 5% concentration.

What is the importance of ensuring the antisera is newly opened?

Ensuring the antisera is newly opened is important because it guarantees the substance is fresh and hasn’t lost potency or been contaminated, which is critical for accurate testing results.

Why is it important to perform the validation process at 2 to 8 degrees Celsius?

The antisera must be stored at 2 to 8 degrees Celsius to maintain its effectiveness and prevent degradation. It should be in cold storage when not in use, ensuring reliable results during testing.

What is the role of the O cells in the validation process?

The O cells act as a control during the validation process. They are used to check for any unexpected reactions, such as in cases of Bombay blood group, where there might be no agglutination with anti-A or anti-B antisera.

What should you do after adding the antisera and cells to the test tubes?

After adding the antisera and cells to the test tubes, you should homogenize each sample thoroughly and then perform centrifugation at 2000 to 3000 RPM for 2 minutes to separate any agglutinated cells.

How long should the centrifugation be performed during the validation process?

Centrifugation should be performed for about 2 minutes at 2000 to 3000 RPM to ensure the agglutination process is properly observed. The duration can vary slightly depending on the specific conditions.

What happens after centrifugation, and how do you observe the results?

After centrifugation, the test tubes should be gently shaken to observe the agglutination. If agglutination occurs, the sample will appear clumpy. If there is no agglutination, the sample will remain clear or turbid.

Why is it necessary to check the expiration dates on antisera during validation?

It is essential to check the expiration dates on antisera to ensure that they are still valid and have not lost their effectiveness. Expired antisera may give inaccurate results, leading to incorrect blood group identification.

What is the significance of labeling and documenting the validation results?

Labeling and documenting the validation results are important for traceability and record-keeping. It ensures that each test is properly logged with the relevant details, such as the date, the person's name, and the validity of the antisera used, making the process transparent and accountable.