Imunohematologi BD: Validasi Reagensia dan Sampel BDRS

Summary

This video provides an in-depth explanation on the process of reagent validation in blood banks, specifically focusing on immunohematology procedures. It covers essential topics such as reagent validation steps, proper handling and storage of reagents, the role of crossmatching, and ensuring the quality of blood samples. Detailed guidance is provided on how to test and validate different reagents and cells, including antisera and blood group reagents. The video emphasizes the importance of maintaining accuracy, quality control, and proper documentation to ensure reliable results for blood transfusion compatibility. The presenter also touches on equipment maintenance, safety protocols, and continuous validation practices.

Takeaways

• 😀 Validation in blood banking ensures reagents are in good condition and react as expected during testing.
• 😀 Reagent validation includes checking expiration dates, completeness, quantity, appearance, and proper storage conditions.
• 😀 A validation checklist must be used for each step of reagent validation to maintain quality control.
• 😀 Validation steps involve determining reagent usability, conducting laboratory tests, reading results, and making conclusions about agglutination.
• 😀 Blood bank reagents such as anti-A, anti-B, and anti-D are validated through crossmatching tests with specific procedures and visual results.
• 😀 Each reagent and blood sample requires proper documentation, including the name of the examiner, the date, and validation results.
• 😀 Agglutination strength is categorized from positive 4 (strong) to negative (no reaction), which helps determine the validity of the reagents.
• 😀 Blood samples for crossmatch testing need to meet quality standards, including proper packaging, labeling, and accurate patient and donor information.
• 😀 During donor blood selection, hemolysis or any signs of blood degradation disqualify the donation for use in testing.
• 😀 Validation of blood typing tools and equipment (like slides, pipettes, and centrifuges) ensures their reliability for accurate results in blood banking processes.

Q & A

What is the purpose of validation in a blood bank?

Validation in a blood bank ensures that reagents used are in good condition and react as expected, ensuring the quality and accuracy of laboratory tests performed.

What should be checked during the validation of reagents in a blood bank?

During validation, the following should be checked: expiration date, reagent completeness, reagent quantity, appearance, compatibility with processes, and proper storage conditions.

What are the steps involved in reagent validation in a blood bank?

The steps include: 1) Determining validation criteria (specificity, label, appearance, usage), 2) Checking expiration dates and completeness, 3) Preparing equipment, 4) Performing the validation test, 5) Reading results, 6) Concluding the test results, and 7) Recommending whether the reagent is suitable for use.

How is the agglutination test performed for blood typing reagents like anti-A, anti-B, and anti-D?

Agglutination tests for anti-A, anti-B, and anti-D are performed by adding 1-2 drops of reagent (e.g., anti-A, anti-B, anti-D) to test tubes with blood samples, centrifuging, and observing if agglutination occurs to determine blood type.

What is the significance of the card used for reagent validation?

The card used for reagent validation serves as a guide to track each step of the validation process, ensuring that every action is recorded, such as reagent application and validation completion.

Why is it important to validate blood samples in a blood bank?

Validating blood samples ensures that the sample is properly labeled, meets quality standards, and contains the correct information before performing blood typing or cross-matching tests.

What are the characteristics of valid donor blood?

Valid donor blood should have clear plasma (no hemolysis), free from contamination or improper handling, and stored under appropriate conditions to maintain blood cell integrity.

What should be checked in blood typing reagents like anti-A, anti-B, and anti-D during validation?

Reagents like anti-A, anti-B, and anti-D should be checked for expiration, completeness, correct labeling, proper storage, and their ability to produce clear agglutination results when mixed with blood samples.

What role do blood crossmatch tests play in blood bank procedures?

Blood crossmatch tests ensure that the donor's blood is compatible with the recipient’s blood, preventing adverse reactions during blood transfusion by checking for agglutination between the donor and recipient blood.

How is the quality of laboratory equipment validated in a blood bank?

The quality of laboratory equipment is validated by ensuring it is properly calibrated, maintained, and functioning correctly, such as centrifuges, pipettes, incubators, and refrigerators.