CSL FK - CSL 1 Pengenalan Mikroskop

Summary

In this video, Selfie Rahmawati, a teaching staff member from the Faculty of Medicine at the University of Lampung, introduces the various parts of a microscope and demonstrates how to use it effectively. She covers essential components such as the base, lenses, light adjustment, and specimen stage, and explains how to properly focus the microscope using coarse and fine adjustments. Additionally, she demonstrates how to handle the microscope, prepare specimens, and clean the equipment after use. The video aims to provide students with clear instructions for proper microscope use in histological studies.

Takeaways

• 🔬 The microscope consists of key parts including the base, arm, head, and illumination system, each serving specific functions.
• 💡 The illumination system has a lamp that provides light and a brightness control to adjust the intensity according to the needs of the observation.
• 🔧 The coarse focus (macro) knob is used for quick focusing, while the fine focus (micro) knob provides precise, detailed adjustments.
• 🧪 The stage holds the specimen and features clips to secure the slide in place, with adjustments for vertical and horizontal movement.
• 👁️ The eyepiece (ocular lens) is used for viewing the specimen, while objective lenses with different magnifications (4x, 10x, 40x, 100x) offer varying levels of detail.
• 🛡️ Immersion oil is used for high magnification (100x) to enhance clarity, and must be cleaned off properly to avoid damage to the lens.
• 🧹 Proper cleaning and maintenance of the microscope, such as using lens tissue, is necessary to prevent lens damage and ensure longevity.
• 💪 When handling the microscope, use both hands: one to hold the arm and the other to support the base to ensure stability during transportation.
• 🧭 The position of the specimen should be adjusted using the stage’s vertical and horizontal controls to align it properly with the light source.
• ⚠️ Always set the objective lens back to the lowest magnification (4x) after use, turn off the lamp, and unplug the microscope before storing it.

Q & A

What are the key parts of a microscope covered in the script?

The key parts of a microscope covered include the base, arm, head, light switch, light adjustment screw, macro and micro focus screws, specimen stage, lamp, filter, diaphragm ring, objective lenses, ocular lenses, condenser, and diaphragm screw.

How do you adjust the light intensity on a microscope?

The light intensity is adjusted using the screw that regulates the brightness of the lamp, with markings indicating the wattage of the light.

What is the function of the macro and micro focus screws on a microscope?

The macro focus screw is used to adjust the focus quickly, while the micro focus screw fine-tunes the focus slowly for more precise adjustments.

What is the proper way to handle and move a microscope?

To handle a microscope, use both hands: the right hand holds the arm of the microscope, and the left hand supports the base to ensure stability while lifting and placing it carefully.

What type of specimen can be observed with a microscope?

A variety of specimens can be observed, such as organ slices that are mounted on a glass slide or blood smears, each requiring different preparation methods.

How do you properly orient a specimen on the microscope stage?

Ensure the specimen is placed the right way up, with the rough side facing upwards for blood smears, and the glass cover facing upwards for organ slices. This prevents the specimen from being inverted.

What is the purpose of immersion oil in microscopy?

Immersion oil is used when observing at high magnifications (100x) to improve focus and clarity by reducing light refraction.

How should the lens be cleaned after using immersion oil?

The lens should be cleaned with a special lens tissue or paper, gently wiping away any immersion oil to prevent damage to the lens.

How do you change the objective lens on a microscope?

To change the objective lens, rotate the lens turret to the desired magnification, starting with the 4x objective for low magnification and switching to higher magnifications as needed.

What precautions should be taken when adjusting the condenser and diaphragm?

Adjust the condenser and diaphragm carefully and slowly to avoid potential damage to the specimen. The condenser height should be adjusted only when necessary, and the diaphragm should be rotated to control the amount of light entering the specimen.