PRAKTIKUM FARMASI FISIKA UJI DISOLUSI TABLET PARACETAMOL

Summary

This video demonstrates a dissolution test using a Type 2 dissolution apparatus to evaluate the disintegration of paracetamol tablets. It covers the step-by-step process, including the preparation of phosphate buffer, the setup of the dissolution apparatus, and the immersion of tablets. The test is conducted at 37°C with 50 rpm for 30 minutes. Samples are taken, filtered, and diluted for spectrophotometric analysis. The concentration of paracetamol is calculated, and the dissolution rate is compared with the pharmacopoeial standards to ensure the tablets meet the required dissolution criteria. The entire process is visually accompanied by music and explanations, concluding with the successful completion of the test.

Takeaways

• 😀 The dissolution test is conducted using a Type II dissolution apparatus with a paddle mechanism.
• 😀 900 mL of phosphate buffer is used as the dissolution medium in the test.
• 😀 The beaker is placed into a water bath as part of the dissolution testing setup.
• 😀 The paddle's axis is positioned at the center of the dissolution beaker, with a gap of 2.5 cm from the beaker's bottom.
• 😀 The dissolution test is set to run at 50 rpm for 30 minutes at 37°C ± 0.5°C.
• 😀 A paracetamol tablet is placed in the dissolution beaker without touching the paddle or the bottom of the beaker.
• 😀 Sampling is done using a sampling tube with its tip positioned between the paddle and the surface of the dissolution medium.
• 😀 A 5 mL sample is taken using a syringe after the dissolution test is complete.
• 😀 The sample is filtered, diluted with phosphate buffer (pH 5.8), and then analyzed using a spectrophotometer.
• 😀 The absorbance of the sample is measured at a wavelength of 243 nm to determine the concentration of dissolved paracetamol.
• 😀 The calculated concentration data is compared to the standard curve to assess the dissolution performance of the tablet, ensuring it meets the requirements of the Indonesian Pharmacopoeia (85% dissolution within 30 minutes).

Q & A

What type of dissolution apparatus is used in this test?

The test uses a dissolution apparatus type 2, which is a paddle type with specific equipment for the procedure.

What is the purpose of the phosphate buffer solution in the dissolution test?

The phosphate buffer solution is used as the dissolution medium to simulate the conditions in the human gastrointestinal tract.

How much phosphate buffer solution is poured into the dissolution vessel?

900 ml of phosphate buffer solution is poured into the dissolution vessel.

What is the optimal temperature for the dissolution process in this test?

The dissolution process is carried out at a temperature of 37 ± 0.5°C.

How is the paddle's position set in the dissolution vessel?

The paddle is positioned so that the axis of the shaft is located in the center of the dissolution vessel, and the distance between the paddle and the bottom of the vessel is approximately 2.5 cm.

What is the speed setting of the paddle during the dissolution process?

The paddle is set to rotate at 50 rpm during the dissolution process.

How long is the dissolution test conducted?

The dissolution test is conducted for 30 minutes.

What is the purpose of the sampling tube in the dissolution apparatus?

The sampling tube is used to collect a sample from the dissolution medium without disturbing the paddle, ensuring accurate sample retrieval.

What method is used to filter the sample before testing?

The sample is filtered using a filter holder equipped with a 0.45-micron filter paper before being transferred to a reaction tube.

How is the concentration of paracetamol measured after dilution?

The concentration of paracetamol is measured by spectrophotometry at a wavelength of 243 nm after the sample is diluted and homogenized.

What is the minimum percentage of paracetamol dissolution required for the tablet to pass the test?

The tablet must dissolve at least 85% of its paracetamol content within 30 minutes to meet the acceptance criteria according to the Indonesian Pharmacopoeia.