Praktikum Farmasi Fisik: Percobaan Uji Disolusi (1) - dibuat sebelum pandemi Covid-19

Summary

The video script details the process of conducting a dissolution test for sodium diclofenac using a phosphate buffer medium. The procedure includes setting up the dissolution apparatus at 30°C, taking samples every 5 minutes for 25 minutes, and analyzing the dissolution profile via spectrophotometry. The process is repeated with five different samples taken at intervals, ensuring comprehensive testing. The script is interspersed with music and light-hearted interactions, making the technical steps more engaging. The testing concludes with the cleaning of equipment after use.

Takeaways

• 😀 The dissolution test uses a phosphate buffer medium for testing the dissolution of sodium diclofenac.
• 😀 The test involves setting up dissolution apparatus with a phosphate buffer and adjusting the temperature and rotation speed.
• 😀 The dissolution apparatus operates at a temperature of 30°C, with a rotation speed of 150 RPM, for a duration of 25 minutes.
• 😀 After reaching a temperature of 38°C, sodium diclofenac is added to the dissolution medium.
• 😀 Samples are taken every 5 minutes to measure the dissolution profile of the drug.
• 😀 The samples are collected in vials and new buffer medium is added to replace the taken samples.
• 😀 The process is repeated at 5-minute intervals for a total of five replicates (5, 10, 15, 20, and 25 minutes).
• 😀 The dissolution profile is analyzed using a spectrophotometric method.
• 😀 After completing the test, all equipment used must be thoroughly cleaned.
• 😀 The test process follows a step-by-step procedure to ensure accuracy and reliability of the results.

Q & A

What is the main procedure discussed in the script?

The script outlines a dissolution test procedure using phosphate buffer to test the dissolution profile of sodium diclofenac in a laboratory setting.

What is the purpose of the phosphate buffer medium in the dissolution test?

The phosphate buffer medium is used to simulate the conditions under which the drug will dissolve, ensuring that the test is consistent and accurate for the dissolution profile of sodium diclofenac.

What equipment is mentioned in the script for the dissolution test?

The script mentions a dissolution apparatus, a dissolution jar, a filter holder, and a spectrophotometer, which are used for carrying out and analyzing the test.

What is the temperature setting for the dissolution test?

The dissolution test is set at a temperature of 30°C for the apparatus and is adjusted to 38°C for the medium before the sodium diclofenac is added.

How often are samples taken during the dissolution test?

Samples are taken every five minutes during the dissolution test to monitor the drug's dissolution profile.

How many replications of the dissolution test are performed?

Five replications of the dissolution test are performed, at the 5th, 10th, 15th, 20th, and 25th-minute marks.

What method is used to analyze the dissolution samples?

The dissolution samples are analyzed using a spectrophotometric method to obtain the dissolution profile of the drug being tested.

What happens to the medium after a sample is taken?

After each sample is taken, new medium is added to the dissolution jar to replace the volume that was removed, maintaining consistent conditions throughout the test.

What should be done after the dissolution apparatus is used?

After the dissolution apparatus is used, it should be thoroughly cleaned to ensure it is ready for future tests and to avoid contamination between tests.

What is the purpose of adjusting the rotation speed of the apparatus?

The rotation speed of the apparatus is set at 150 RPM to ensure proper mixing and movement of the medium, which helps in the accurate dissolution of the drug during the test.