Praktikum Patologi Klinik - Pemeriksaan HB Sahli

Summary

This video script outlines the step-by-step procedure for performing a hemoglobin (HB) test using the Sahli method. It starts with preparing the necessary solutions, including 0.1N HCl, followed by the collection of a blood sample and mixing it with the HCl solution in a Sahli tube. After waiting for a reaction, the solution is diluted with Aquatest, and the color is compared to a standard. The resulting hemoglobin concentration is calculated using a correction factor. The video concludes with a prayer of gratitude, emphasizing the simplicity and accuracy of the process.

Takeaways

• 😀 The procedure starts with a greeting and a prayer: Bismillahirohmanirohim.
• 😀 The first step involves using a 0.1 N HCl solution, which is drawn with a pipette into a Sahli tube.
• 😀 The HCl solution is added into the Sahli tube up to a specific line, indicated as 0% on the scale.
• 😀 A second pipette is used to draw a blood sample and transfer it into the Sahli tube.
• 😀 The blood is mixed with the HCl solution to create a homogeneous mixture.
• 😀 After mixing, the solution is left to stand for 10 minutes.
• 😀 The Sahli tube is placed in a brown-colored standard tube for stability during the waiting period.
• 😀 After 10 minutes, the solution is diluted with an aquatest solution, which is added until the color matches the standard tube color.
• 😀 The solution's color is compared to a reference standard, and adjustments are made to match the hue as closely as possible.
• 😀 The factor correction from the standard is noted and used to calculate the final hemoglobin value.
• 😀 The final hemoglobin concentration is determined by multiplying the observed reading with the correction factor, completing the test.

Q & A

What is the first step in the Hemoglobin testing process using the Sahli method?

The first step is to greet the participants and introduce the materials needed, including the 0.1 N HCl solution, Sahli pipet, and Sahli tube.

How is the 0.1 N HCl solution used in the procedure?

The 0.1 N HCl solution is drawn using a pipet and transferred into the Sahli tube up to the marked line.

What is the purpose of using the Sahli pipet in the procedure?

The Sahli pipet is used to draw a blood sample, which is then added to the Sahli tube containing the HCl solution.

How is contamination avoided during the procedure?

Excess blood is cleaned from the outside of the pipet and Sahli tube to prevent contamination and ensure accurate results.

What is done after the blood sample is added to the Sahli tube?

The blood and HCl solution are mixed until homogeneous, and the mixture is allowed to sit for 10 minutes.

What is the purpose of the 10-minute wait during the procedure?

The 10-minute wait allows the blood to react with the HCl solution, ensuring proper mixing and preparation for the next steps.

What happens after the 10-minute waiting period?

After 10 minutes, the Sahli tube is placed in a color comparator box, and aquatest is added to dilute the mixture until the color matches the standard comparator.

How is the hemoglobin level determined in the Sahli method?

Once the color of the mixture matches the standard comparator, the meniscus level is observed, and the reading is multiplied by the correction factor to determine the hemoglobin level.

What is the significance of the correction factor in the Sahli method?

The correction factor is used to adjust the observed reading from the meniscus, ensuring that the final hemoglobin level measurement is accurate.

How does the procedure conclude?

The procedure concludes by noting the hemoglobin level result and thanking the participants with a closing greeting.