Pemeriksaan Feses/Telur Cacing Metode Sedimentasi

Summary

This video provides a step-by-step guide for performing a fecal examination using the sedimentation method. The process begins with handwashing, followed by preparing the necessary tools such as aquades, test tubes, and a microscope. The macroscopic analysis involves checking the color and odor of the feces. The microscopic analysis involves centrifuging the sample, adding aquades, and observing the sediment under the microscope. The procedure ensures accurate results by thoroughly cleaning and preparing the sample, making it ready for analysis under a 10x to 40x magnification.

Takeaways

• 😀 Introduction by Miftahul, an Atel M, who will demonstrate the fecal sedimentation examination method.
• 😀 The first step in the procedure is to wash hands using the 6-step handwashing method.
• 😀 Key materials required for the process include aquades, centrifuge tubes, stirring rods, glass slides, and a microscope.
• 😀 The macroscopic examination of the feces involves checking the color and odor of the sample.
• 😀 After the macroscopic examination, the process proceeds to the microscopic examination of the feces.
• 😀 Begin by placing the fecal sample into a centrifuge tube.
• 😀 Add aquades to the centrifuge tube and mix the feces with a stirring rod.
• 😀 Fill the centrifuge tube up to one-quarter of its capacity with aquades, and then centrifuge at 2000 RPM for 5 minutes.
• 😀 Repeat the centrifugation process until the water in the tube becomes clear or non-turbid.
• 😀 After centrifugation, discard the liquid and collect the fecal sediment to place onto a glass slide, covered with a cover slip for observation under a microscope.

Q & A

What is the first step before starting the feces examination procedure?

The first step is to wash your hands using the 6-step handwashing procedure to ensure cleanliness and avoid contamination.

What are the essential tools and materials needed for the feces examination?

The tools and materials needed include aquades (water), a centrifuge tube, a stirring rod, a glass slide, a cover slip, a microscope, and the feces sample.

What is the purpose of the macroscopic examination in feces analysis?

The macroscopic examination involves evaluating the color and odor of the feces, which can provide initial insights into the sample's condition or any abnormalities.

What should you do after preparing the feces sample for microscopic examination?

After preparing the sample, you should add the feces to the centrifuge tube, then add aquades (water) and mix the contents to ensure proper homogenization.

How long should the centrifuge tube be spun during the sedimentation process?

The centrifuge tube should be spun at a speed of 2000 RPM for 5 minutes to help separate the feces components.

What should you do if the water in the centrifuge tube is still cloudy after the first centrifugation?

You should repeat the centrifugation process until the water becomes clear or no longer cloudy, indicating that the sedimentation is complete.

What should be done after centrifuging the feces sample?

After centrifuging, the supernatant (the clear water) should be discarded, and the feces sediment should be transferred onto a glass slide and covered with a cover slip for microscopic observation.

At what magnification should the feces sample be observed under the microscope?

The sample should be observed under the microscope using an objective lens magnification of 10x to 40x.

What is the significance of observing the sample under the microscope?

The microscopic examination helps identify various elements in the feces, such as parasites, bacteria, or other abnormalities, which are not visible to the naked eye.

What are the final steps after preparing the feces slide for observation?

Once the slide is prepared with the feces sediment, it is covered with a cover slip, and the sample is ready for observation under the microscope.