Protein structure | Primary | Secondary | Tertiary | Quaternary
Summary
TLDRThis video script delves into the intricate structure of proteins, outlining their primary, secondary, tertiary, and quaternary structures. It explains how a protein's primary structure is a sequence of amino acids linked by peptide bonds, which can be determined through gene or protein sequencing. The secondary structure involves alpha helices and beta sheets, stabilized by hydrogen bonds. The tertiary structure refers to the three-dimensional arrangement of the protein, influenced by various bonds and interactions. Finally, the quaternary structure describes the assembly of multiple tertiary structures into larger protein complexes, essential for cellular function.
Takeaways
- 𧬠The primary structure of a protein is its linear sequence of amino acids, which can be represented in one or three-letter form.
- π Peptide bonds link amino acids together in the primary structure, with the sequence typically written from the N-terminal to the C-terminal.
- 𧬠Sequencing the gene or the protein itself can provide information about the primary structure.
- π The secondary structure of proteins involves folding due to hydrogen bonding, leading to structures like alpha helices or beta sheets.
- π Alpha helices are common and stabilized by hydrogen bonds between the nitrogen's hydrogen and the carbon's oxygen, forming a spiral shape with side chains outwards.
- π Beta sheets are another type of secondary structure, where hydrogen bonds form between different polypeptide chains or within the same chain, arranged either parallel or antiparallel.
- π The tertiary structure refers to the three-dimensional arrangement of a protein in space, influenced by bonds like disulfide bonds, hydrophobic interactions, and ionic interactions.
- π€ Proteins with tertiary structures can interact to form quaternary structures, such as dimers, trimers, and tetramers.
- π§ Most proteins in the cell have a quaternary structure, indicating that they can form complexes with other proteins.
- π« The presence of certain amino acids like proline can disrupt the helix structure, making it unstable.
Q & A
What are the four levels of protein structure?
-The four levels of protein structure are primary, secondary, tertiary, and quaternary.
What is the primary structure of a protein?
-The primary structure of a protein is the linear sequence of amino acids present in the protein. It is written from the end terminal of the first amino acid to the C-terminal of the last amino acid.
How are amino acids in a protein linked together?
-Amino acids in a protein are linked together by peptide bonds.
How is the information about the primary structure of a protein obtained?
-The information about the primary structure of a protein can be obtained by sequencing the gene that encodes the protein or by sequencing the protein itself.
What is the secondary structure of a protein?
-The secondary structure of a protein refers to the local folding of the peptide chain due to hydrogen bonding, resulting in structures like alpha helices and beta sheets.
What is an alpha helix and how is it stabilized?
-An alpha helix is the most common secondary structure of a protein, where the protein attains a spiral shape with the side chain of amino acids remaining outwards. It is stabilized by hydrogen bonding between the hydrogen of nitrogen and the oxygen attached to the carbon.
What is a beta sheet and how does it differ from an alpha helix?
-A beta sheet is another type of secondary structure where hydrogen bonds are formed between two different polypeptide chains or the same polypeptide chain, unlike the alpha helix where hydrogen bonding occurs within the same peptide chain.
What is the tertiary structure of a protein?
-The tertiary structure of a protein is the three-dimensional arrangement of the protein in space, which involves other bonds like disulfide bonds, hydrophobic interactions, and ionic interactions.
What is the quaternary structure of a protein?
-The quaternary structure of a protein is formed when proteins with tertiary structure interact with other tertiary structure proteins to form dimers, trimers, tetramers, and so on.
How do amino acids like proline affect the alpha helix structure?
-The presence of amino acids like proline can make the alpha helix unstable and disrupt the helix due to its unique structure that hinders the formation of hydrogen bonds necessary for stabilizing the helix.
What are the typical dimensions of each turn in an alpha helix?
-Each turn of the alpha helix has about 3.6 amino acids and is at a distance of 0.54 nanometers.
Outlines
𧬠Protein Structure Basics
This paragraph introduces the fundamental concept of protein structure, explaining the four levels of protein organization: primary, secondary, tertiary, and quaternary. The primary structure is described as the linear sequence of amino acids linked by peptide bonds, which can be determined by gene or protein sequencing. The secondary structure involves the folding of the peptide chain into specific patterns like alpha helices and beta sheets, driven by hydrogen bonding between the peptide bonds. Alpha helices are characterized by their spiral shape with amino acid side chains pointing outwards, stabilized by hydrogen bonds between nitrogen and oxygen atoms. Beta sheets, on the other hand, involve hydrogen bonding between different polypeptide chains, which can be arranged either parallel or antiparallel. The paragraph also touches upon the tertiary structure, which refers to the overall three-dimensional arrangement of the protein, stabilized by various types of bonds and interactions, and the quaternary structure, where multiple tertiary structures interact to form larger protein complexes.
Mindmap
Keywords
π‘Protein Structure
π‘Primary Structure
π‘Peptide Bond
π‘Alpha Helix
π‘Beta Sheet
π‘Hydrogen Bonding
π‘Tertiary Structure
π‘Quaternary Structure
π‘Amino Acids
π‘Proline
π‘Disulfide Bond
Highlights
Proteins have a hierarchical structure with primary, secondary, tertiary, and quaternary levels.
Primary structure is the linear sequence of amino acids, which can be represented in one or three-letter form.
Amino acids in a protein are connected by peptide bonds.
The direction of primary structure notation is from the N-terminal to the C-terminal of the protein.
Information on the primary structure can be obtained by gene or protein sequencing.
Secondary structure arises from hydrogen bonding between the peptide bonds, leading to alpha helix or beta sheet formations.
Alpha helix is the most common secondary structure, characterized by a spiral shape with hydrogen bonding stabilization.
Hydrogen bonds in alpha helix involve the hydrogen of nitrogen and the oxygen of carbon, with each turn having 3.6 amino acids.
Presence of proline can disrupt the alpha helix stability.
Beta sheet is another secondary structure with hydrogen bonds formed between different polypeptide chains.
Beta sheets can be arranged in parallel or antiparallel fashions.
Tertiary structure refers to the three-dimensional arrangement of a protein, stabilized by various interactions.
Disulfide bonds, hydrophobic interactions, and ionic interactions contribute to the stability of the tertiary structure.
Quaternary structure is formed when multiple tertiary structures interact to form larger protein complexes.
Most proteins in the cell have a quaternary structure.
The hierarchical structure of proteins is crucial for their function and stability.
Transcripts
hey everyone put back a mr. basics here
let's talk about structure of protein so
the proteins have a primary secondary
tertiary and a quaternary structure
let's talk about each of them primary
structure of protein primary structure
of protein is a linear sequence of amino
acids present in a protein this linear
sequence of amino acid is either written
in a three letter or a one letter form
now these amino acids are attached with
each other by a peptide bond now the
primary structure of protein is always
written from the end terminal of the
first amino acid to the C terminal of
the last amino acid
the information of the primary structure
protein can be obtained by sequencing
the gene that encodes the protein this
information can also be obtained by
sequencing the protein itself secondary
structure of protein now in the primary
structure of protein the amino acids are
linked by peptide bond and the peptide
bond has an oxygen attached with a
carbon and a nitrogen attached with the
hydrogen both oxygen and hydrogen have
the ability to form hydrogen bond and
this results in folding of the peptide
chain either an alpha helix or neither a
sheet
let's talk about alpha helix alpha helix
is the most common secondary structure
of protein and the structure the protein
attains a spiral shape such that the
side chain of amino acid remains
outwards the alpha helix is stabilized
by hydrogen bonding this hydrogen
bonding is formed between the hydrogen
of nitrogen and the oxygen attached with
the carbon individual hydrogen bonding
are of course weak but collectively they
are strong enough to stabilize the
spiral structure all the peptide bond
except the first and the last
participate in the hydrogen bonding each
turn of the helix has three point six
amino acids at a distance of 0.5 4
nanometers presence of amino acids like
prolene can make the helix unstable and
destruct the helix the beta sheet rena
sheet is a second type of secondary
structure of protein now in alpha helix
the hydrogen bonding was between the
same peptide chain while in me Rashid
the hydrogen bond is formed between the
two different polypeptide or the same
polypeptide chain the beta sheet can be
eating arranged in a parallel or
antiparallel fashion
tertiary structure of protein
arrangement of protein in the
three-dimensional space is the tertiary
structure of protein
the tertiary structure will also have
other bonds like disulfide bond
hydrophobic interactions and ionic
interactions quaternary structure of
protein the protein having tertiary
structure can further interact with
other tertiary structure proteins to
either form dimers trimers tetra Mars
and so on the structure so form is
called quaternary structure most of the
proteins in the cell have the quaternary
structure
[Music]
5.0 / 5 (0 votes)