Praktikum Uji Enzim Katalase (pengaruh pH dan suhu terhadap kerja enzim)

Summary

In this experiment, Syahid demonstrates the effect of temperature and pH on the catalase enzyme using chicken liver. The experiment involves testing the enzyme's reaction under five conditions: normal, acidic, basic, hot, and cold. By observing the production of bubbles and flames, Syahid explains how catalase breaks down hydrogen peroxide into water and oxygen. The results show that the enzyme works best in normal conditions, with reduced activity in both extreme temperatures and acidic environments. This insightful experiment highlights the crucial role of pH and temperature in enzyme function.

Takeaways

• 😀 The experiment demonstrates the role of the catalase enzyme in breaking down hydrogen peroxide (H2O2) using chicken liver.
• 😀 Tools and materials needed include chicken liver, H2O2, ice cubes, HCL, NaOH, methylated spirits, test tubes, and more.
• 😀 The liver is cut into small pieces and tested under five different conditions to observe the enzyme's activity: normal, acidic, basic, hot, and cold.
• 😀 The experiment uses a beaker, water bath, and tripod to create temperature and pH conditions for the liver and hydrogen peroxide reaction.
• 😀 The catalase enzyme’s reaction is influenced by temperature and pH, which can affect the rate of H2O2 breakdown.
• 😀 Normal conditions (room temperature and neutral pH) result in many bubbles and flames, indicating active enzyme activity.
• 😀 Under acidic conditions (HCl), fewer bubbles and flames are produced, showing reduced enzyme activity.
• 😀 Under basic conditions (NaOH), the enzyme produces more bubbles but fewer flames compared to normal conditions.
• 😀 Hot temperatures slow down the catalase enzyme’s effectiveness, producing fewer bubbles and flames due to enzyme denaturation.
• 😀 Cold temperatures also hinder the enzyme’s activity, with fewer bubbles and little to no flame produced.
• 😀 The conclusion of the experiment is that the catalase enzyme works best under normal temperature and pH, efficiently breaking down hydrogen peroxide into water and oxygen.

Q & A

What is the purpose of this experiment?

The purpose of this experiment is to test the activity of the catalase enzyme using chicken liver and observe how factors such as temperature and pH affect its function in breaking down hydrogen peroxide.

What are the key materials used in this experiment?

The key materials used include a stick, ice cubes, chicken liver, a dropper, a beaker, water bath, tripod, H2O2 (hydrogen peroxide), NaOH (sodium hydroxide), HCl (hydrochloric acid), methylated spirits, test tube rack, knife, plates, and tongs.

How are the test conditions set up in this experiment?

Five test tubes are prepared with chicken liver, each subjected to different conditions: normal, acidic (HCl added), basic (NaOH added), hot, and cold temperatures.

What role does methylated spirits play in the experiment?

Methylated spirits are used to heat one of the beakers to create a hot condition for the test tubes, aiding in the observation of the catalase enzyme's activity at elevated temperatures.

What effect does cold temperature have on the catalase enzyme?

Cold temperatures slow down the activity of the catalase enzyme, resulting in fewer bubbles and less flame production, as enzymes function less efficiently at low temperatures.

Why does the catalase enzyme behave differently in hot conditions?

In hot conditions, the catalase enzyme becomes denatured, meaning it loses its functional shape and cannot effectively break down hydrogen peroxide, leading to fewer bubbles and flames.

How does the catalase enzyme respond in an acidic environment?

In an acidic environment, where HCl is added, the enzyme produces fewer bubbles and flames, as the enzyme is less effective in acidic conditions, causing a decrease in its activity.

What happens when the catalase enzyme is exposed to a basic environment?

In a basic environment, where NaOH is added, the enzyme produces more bubbles, although the flames remain small, as the enzyme can still work at a basic pH, but not at its optimal level.

What is the optimal condition for the catalase enzyme to function?

The catalase enzyme works best in normal conditions, where the liver is at an optimal temperature and pH, producing the highest amount of bubbles and flames during the reaction.

What conclusion can be drawn from the results of this experiment?

The experiment shows that the catalase enzyme's ability to break down hydrogen peroxide is significantly influenced by temperature and pH, with optimal activity occurring at normal conditions and reduced activity in extreme temperatures or pH levels.