Praktikum Biofarmasetika: Uji Difusi

Summary

In this video, a biofarmasetika instructor explains the procedure for conducting in vitro diffusion testing using a specialized diffusion apparatus. The process involves setting up a waterbath, preparing the diffusion cells with a sample and membrane, and ensuring all components are correctly positioned. The instructor details how to control temperature, eliminate air bubbles, and take samples at specific intervals. Following this, the samples undergo dilution and analysis using a Shimadzu spectrophotometer to determine their concentration. The video provides a step-by-step guide on performing the test, making it a valuable resource for students and professionals in biofarmasetika.

Takeaways

• 😀 The video covers the process of determining pharmacokinetic parameters for intravenous drugs.
• 😀 The primary focus is on performing an in vitro diffusion test using a diffusion cell and a water bath.
• 😀 A water bath is used to regulate the temperature for the diffusion process, which should match human body temperature (37°C).
• 😀 The diffusion cell needs to be prepared properly by ensuring all equipment is connected and functioning, including the water bath.
• 😀 Proper preparation includes checking that the diffusion cell's membrane is in place and that the sample is correctly placed within the cell.
• 😀 The test requires adding a phosphate buffer solution as the medium in the diffusion cell and ensuring there are no air bubbles in the system.
• 😀 A magnetic stirrer is placed in the diffusion cell to ensure proper mixing during the experiment.
• 😀 Samples are taken from the diffusion cell at specified intervals using a micropipette, and the sample concentration is determined using a calibration curve.
• 😀 After sample collection, the sample is diluted with the same medium and mixed thoroughly before analysis.
• 😀 The concentration of the sample is measured using a spectrophotometer after calibration, ensuring all parameters are set correctly.
• 😀 The final data from the spectrophotometer provides the necessary concentration readings to assess the diffusion process.

Q & A

What is the main topic of the video?

The main topic of the video is the determination of pharmacokinetic parameters for intravenous administration, specifically focusing on in vitro diffusion testing using a diffusion apparatus.

What is the purpose of the waterbath in the diffusion apparatus?

The waterbath is used to regulate the temperature of the diffusion system, ensuring it matches the body's natural temperature (approximately 37°C) for accurate in vitro testing.

How is the temperature controlled during the experiment?

The temperature is controlled by turning on the waterbath and adjusting it until the digital display shows 37°C, mimicking the temperature of the human body for in vitro testing.

What is the role of the diffusion apparatus in the experiment?

The diffusion apparatus is used to simulate the diffusion process in the body, allowing the testing of drug release or absorption in a controlled, laboratory setting using various mediums.

What should be done before starting the diffusion test?

Before starting, ensure that the waterbath is connected to a power source, turned on, and set to the desired temperature. Also, check that the diffusion apparatus and membranes are properly prepared.

What is the purpose of using a magnetic stirrer in the diffusion cell?

The magnetic stirrer is used to ensure that the diffusion medium inside the cell is continuously stirred, helping to maintain a consistent environment for the diffusion process.

How is a sample collected during the diffusion experiment?

A sample is collected by using a micropipette to withdraw a specific volume of the diffusion medium from the diffusion cell at regular intervals, ensuring it corresponds to the desired concentration.

What happens after the sample is collected?

After collecting the sample, it is placed in a volumetric flask for dilution with phosphate buffer. The mixture is then homogenized before the concentration is measured using a spectrophotometer.

How is the concentration of the sample measured?

The concentration of the sample is measured by placing it in a spectrophotometer. The absorbance is recorded, and the concentration is determined based on a calibration curve.

What is the final step in analyzing the diffusion test results?

The final step involves analyzing the spectrophotometric data, noting the wavelength and absorbance values to determine the concentration of the sample, which can be used to assess the diffusion characteristics.