Praktikum Instrumen: Spektrofotometri Inframerah

Summary

This video provides a step-by-step guide to infrared spectrophotometry (FT-IR), explaining its importance in pharmaceutical analysis. The process demonstrates how this technique is used to identify and assess the purity of both liquid and solid samples, such as glycerol and salicylic acid. The tutorial includes instructions on using the FT-IR ATR apparatus, cleaning the equipment, preparing samples, and analyzing spectral data. The session concludes with a demonstration of both liquid and solid sample analysis, highlighting the practical applications of infrared spectrophotometry for quality control and research in the pharmaceutical field.

Takeaways

• 😀 Spectrophotometry in infrared is a qualitative analytical method widely used in pharmaceutical analysis.
• 😀 It is useful for identifying samples or mixtures and determining the purity of a sample.
• 😀 Both solid and liquid samples can be analyzed using infrared spectrophotometry.
• 😀 The FT-IR (Fourier Transform Infrared) spectrometer with ATR (Attenuated Total Reflection) is used for the practical demonstration.
• 😀 The experiment starts with preparing the equipment by logging into the software and cleaning the crystal with lens paper soaked in 70% alcohol.
• 😀 The first sample used in the experiment is glycerol, which is analyzed by placing a few drops on the ATR crystal.
• 😀 For liquid samples like glycerol, there is no need to press the sample; the device analyzes it directly after placement.
• 😀 After the spectrometer reads the sample, the spectrum can be analyzed by breaking down the data and checking the wave numbers.
• 😀 The second sample, salicylic acid (a solid), is analyzed after cleaning the crystal with alcohol and placing the solid on the ATR crystal.
• 😀 After reading the spectrum of the solid sample, similar steps are followed to extract wave number data for analysis.
• 😀 The practical session emphasizes cleaning the equipment thoroughly between samples to avoid cross-contamination and ensure accurate readings.

Q & A

What is the main purpose of using infrared spectrophotometry in pharmaceutical analysis?

Infrared spectrophotometry is primarily used for qualitative analysis to identify samples or mixtures and to assess the purity of a sample.

What types of samples can be analyzed using FT-IR spectroscopy?

Both solid and liquid samples can be analyzed using FT-IR spectroscopy.

What is the first step in preparing the FT-IR ATR device for sample analysis?

The first step is to open the FT-IR ATR software, enter the password, and log in before clicking 'start'.

How is the crystal of the FT-IR ATR cleaned before analysis?

The crystal is cleaned with a lens tissue paper that is dipped in 70% alcohol, not with regular tissue, to ensure no contamination.

What should be done after cleaning the crystal of the FT-IR ATR?

After cleaning the crystal, click 'next' to allow the device to check the background of the room's air.

What is the proper procedure for analyzing a liquid sample, such as glycerol, using FT-IR ATR?

For liquid samples, a few drops are placed in the center of the ATR crystal. Ensure the liquid covers the entire diamond layer, but do not press the sample. After that, click 'next' to start the spectrum reading.

How do you interpret the wave numbers in the FT-IR spectrum?

After obtaining the spectrum, you can break it down and review the peaks to identify the wave numbers. These wave numbers correspond to specific molecular vibrations.

What steps must be followed before analyzing a solid sample, like salicylic acid?

First, clean the ATR crystal to remove any remnants of the previous sample. Then, place a small amount of the solid sample onto the crystal, ensuring it covers the entire diamond layer, and proceed by clicking 'next'.

What is the purpose of cleaning the ATR crystal between analyzing different samples?

Cleaning the ATR crystal between samples is crucial to avoid cross-contamination and to ensure that the analysis of the next sample is accurate and reliable.

Why is it important to analyze both liquid and solid samples in this practical session?

Analyzing both liquid and solid samples provides practical experience with different types of sample preparation and analysis techniques, enhancing the overall understanding of FT-IR spectroscopy in pharmaceutical applications.