Metode Simplisia dengan Perkolasi

STIKES Notokusumo Yogyakarta
27 Dec 202308:22

Summary

TLDRThis video explains the percolation extraction method used in pharmacy to extract active compounds from plant materials, with a focus on Pegagan (Centella Asiatica). The process involves wetting the powdered plant material with ethanol, followed by placing it in a percolator. The solvent then flows through the material, extracting compounds over a 24-hour period. Advantages of the method include efficient separation of extract and solids, while challenges include the need for large amounts of solvent and the possibility of uneven contact between the solvent and material. The method is a practical, continuous extraction technique commonly used in pharmaceutical applications.

Takeaways

  • 😀 The percolation method is used for extracting active compounds from herbal powders by passing a solvent through them.
  • 😀 The main equipment used for the percolation process includes a percolator, separator funnel, and an appropriate solvent like ethanol (70%).
  • 😀 Simplisia (dried plant powder) is first moistened before the percolation process, a step known as maceration.
  • 😀 For percolation, 150 grams of simplisia is mixed with 75 ml of ethanol to wet the powder, but not completely submerge it.
  • 😀 The percolator is prepped with a cotton layer, a filter paper, and the moistened simplisia before the solvent is added.
  • 😀 During percolation, the solvent moves through the simplisia, dissolving active compounds as it flows down, aided by gravity.
  • 😀 The percolation process is adjusted by pressing the simplisia slightly to control the speed of solvent flow.
  • 😀 If the percolation slows down or stops, the simplisia should be loosened or replaced with a slightly coarser powder.
  • 😀 Solvent should be added to the percolator until there is at least a 1 cm layer above the simplisia to ensure effective extraction.
  • 😀 The percolator should drip at a rate of approximately 1 ml per minute or 20 drops per minute for optimal extraction efficiency.
  • 😀 After 24 hours of percolation, the extract can be further processed using a rotary evaporator, followed by biological activity testing.

Q & A

  • What is the primary purpose of percolation extraction in this process?

    -The primary purpose of percolation extraction is to extract active compounds from plant materials (simplisia) by passing a solvent, like ethanol, through the material, ensuring efficient extraction without excessive saturation.

  • What are the main tools used for percolation extraction?

    -The main tools used are a percolator, separating funnel, beaker, and filter paper. Additionally, ethanol 70% is used as the solvent, and the simplisia (plant material) is placed in the percolator.

  • How is the simplisia prepared before percolation begins?

    -Before percolation, the simplisia is wetted with ethanol to ensure even extraction. This is done by mixing 150 grams of simplisia with 75 ml of ethanol, ensuring that the simplisia is dampened, not soaked.

  • What is the purpose of the wetting process in the percolation method?

    -The wetting process (also called maceration) helps to hydrate the simplisia, allowing for better solvent penetration and preventing clumping of the plant material, which ensures more efficient extraction.

  • What happens if the solvent is added too quickly or too slowly during percolation?

    -If the solvent is added too quickly, the active compounds may not be properly extracted, leading to incomplete extraction. If added too slowly, the process becomes inefficient and may lead to evaporation losses.

  • What is the optimal flow rate for the solvent during percolation?

    -The optimal flow rate for the solvent is approximately 1 milliliter per minute or 20 drops per minute. This ensures that the solvent moves through the simplisia at a rate that facilitates proper extraction.

  • What could happen if the percolator is too tightly packed?

    -If the percolator is packed too tightly, the solvent may not flow properly, causing it to be too slow or even block the flow completely. This can be corrected by loosening the packing and adjusting the solvent flow.

  • What is one advantage of using percolation over other extraction methods?

    -One advantage of percolation is that it continuously passes the solvent through the plant material, preventing saturation and ensuring that the active compounds are thoroughly extracted without additional steps like filtration.

  • What are some disadvantages of the percolation method?

    -Some disadvantages include the need for a large amount of solvent, as the solvent must be constantly replenished, and the contact between the solvent and the simplisia may not be as uniform as other methods, such as reflux extraction.

  • What is the next step after percolation is completed?

    -After percolation, the extract is typically subjected to concentration using a rotary evaporator. Then, biological activity testing may be conducted to evaluate the effectiveness of the extracted compounds.

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Related Tags
Herbal ExtractionPharmaceuticalsPercolation MethodSimplisiaEthnopharmacologyExtraction TechniquesPharmacy EducationScience ProcessHerbal MedicineLaboratory Practices