Materi Fitokimia - Praktikum Ekstraksi Metode Maserasi

Rani Rubiyanti
16 Mar 202110:39

Summary

TLDRThis video outlines a step-by-step guide for extracting compounds from Jati Belanda leaves using a non-heating extraction method. The process involves finely grinding the leaves, mixing with a solvent, and allowing it to rest for 3-5 days, with regular stirring. After multiple rounds of soaking and filtering, the mixture is further processed using a rotary evaporator to separate the extract. The method preserves heat-sensitive compounds, making it ideal for extracting substances that degrade with heat. The technique is suitable for creating potent extracts while protecting the active compounds.

Takeaways

  • 😀 Grind the Belanda teak leaves using a blender, then sift with a mesh sieve of 22 mesh size.
  • 😀 Weigh the finely ground material, using 100 grams for the extraction process.
  • 😀 Place the weighed material into a container and add a solvent to fully moisten it.
  • 😀 Mix the material thoroughly, following a ratio of 1 part of the herb to 10 parts of solvent.
  • 😀 Seal the container, keeping it in a dark place for 3-5 days and stir the mixture regularly.
  • 😀 Ensure the container is shielded from light and air by covering it with black plastic.
  • 😀 Continue stirring the mixture every 6 hours during the 3-5 day period.
  • 😀 After extraction, filter the mixture using flannel cloth to separate small herb particles.
  • 😀 Repeat the soaking process with the same solvent up to three times for thorough extraction.
  • 😀 Use filter paper to perform an additional filtration to ensure no fine particles remain.
  • 😀 After completing the extraction and filtration, use a rotary evaporator to remove excess solvent.
  • 😀 Once the liquid extract is obtained, use a water bath to further evaporate the water content.
  • 😀 This method of extraction is referred to as cold extraction, ideal for heat-sensitive compounds.

Q & A

  • What is the first step in the extraction process described in the script?

    -The first step is to finely grind the Jati Belanda leaves using a blender and then sift them to a size of 22 mesh.

  • How much Jati Belanda leaf is needed for the extraction process?

    -100 grams of the ground and sifted Jati Belanda leaves are needed for the extraction.

  • What is the correct ratio of leaves to solvent for the extraction?

    -The correct ratio is 1 part of the leaves to 10 parts of solvent.

  • How long should the mixture of leaves and solvent be left to steep?

    -The mixture should be left to steep for 3-5 days in a dark place, with periodic stirring every 6 hours.

  • Why is it important to cover the container with black plastic?

    -The black plastic is used to protect the mixture from light, air, and moisture, which could affect the extraction process.

  • What tool is used to filter the mixture after the steeping process?

    -A flannel cloth is used to filter the mixture and separate the solid particles from the liquid extract.

  • How many times should the mixture be soaked with the same solvent?

    -The mixture should be soaked and filtered up to three times using the same solvent.

  • What are the key steps involved in the solvent removal process?

    -The solvent is first removed using a rotary evaporator, followed by further evaporation using a water bath to remove any remaining water content.

  • What is the purpose of using a rotary evaporator in the process?

    -The rotary evaporator is used to efficiently remove most of the solvent, leaving behind a concentrated liquid extract.

  • Why is this extraction method referred to as a cold extraction process?

    -This method is considered a cold extraction because the samples are not exposed to heat, which is ideal for extracting heat-sensitive compounds.

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Related Tags
Extraction MethodJati BelandaCold ExtractionNatural RemediesHerbal ScienceSimplisiaExtraction ProcessHeat-sensitive CompoundsBotanical ResearchDIY Extraction