Penelitian : Uji Aktivitas Analgetik dan Antipiretik pada Mencit Putih

Summary

This video outlines an experimental study investigating the analgesic and antipyretic properties of ethanol extract from rambutan seeds on mice. The research aims to observe the reduction of fever and pain in mice by administering varying doses of the extract and comparing them to control groups (Paracetamol and NaCl). The procedure includes inducing fever with a DPT vaccine and measuring temperature changes at various time intervals. The experiment also tests pain response using acetic acid induction. The study's results will help assess the potential therapeutic use of rambutan seed extract for fever and pain management.

Takeaways

• 😀 The research aims to test the analgesic and antipyretic effects of ethanol extract from rambutan seeds in mice.
• 😀 Mice with an average weight of 20 grams are used as test subjects, and their initial body temperature is measured before treatment.
• 😀 Fever is induced in the mice using a DPT vaccine injected intramuscularly.
• 😀 Mice are divided into different experimental groups: negative control (NaCl), positive control (Paracetamol), and three groups receiving varying doses of rambutan seed extract.
• 😀 After inducing fever, the body temperature of the mice is monitored at 15, 30, 60, and 90 minutes to observe the antipyretic effect.
• 😀 The analgesic effect is tested by injecting 1% acetic acid to irritate the stomach, followed by observing the writhing responses of the mice.
• 😀 The materials used include oral sondes, digital thermometers, Paracetamol, and rambutan seed extract.
• 😀 Different doses of rambutan seed extract are administered orally to test its efficacy in reducing fever and pain.
• 😀 The results of temperature measurements and writhing responses are recorded to evaluate the effects of the treatments on fever and pain reduction.
• 😀 The data analysis involves calculating the percentage of pain protection and fever reduction, comparing the results with control groups.

Q & A

What is the main purpose of the study described in the transcript?

The main purpose of the study is to test the analgesic and antipyretic activities of ethanol extract from rambutan seeds on mice, specifically to evaluate the reduction of fever and pain responses in animals.

How were the animals prepared for the study?

The mice were weighed to ensure a consistent average weight of 20 grams, and their initial temperature was measured before starting the induction process.

What was used to induce fever in the mice?

Fever was induced using a DPT (Diphtheria, Pertussis, and Tetanus) vaccine, administered intramuscularly at a dose of 0.2 mL per 20 grams of body weight.

How was the effectiveness of the treatment measured?

The effectiveness of the treatment was measured by monitoring the rectal temperature of the mice at various time points after administering the extract, including 15, 30, 60, 90, 120, and 180 minutes.

What were the different control groups used in the experiment?

The experiment included a negative control group (NaCl), a positive control group (Paracetamol at 500 mg), and three groups with varying doses of the ethanol extract from rambutan seeds.

What types of substances were used in the study?

The substances used in the study included Paracetamol (500 mg), NaCl (for the negative control), ethanol extract from rambutan seeds at different concentrations, and the DPT vaccine to induce fever.

How were the doses of the rambutan seed extract calculated?

The doses of the rambutan seed extract were calculated based on the body weight of the mice. For example, the first dose was 75 mg per 20 grams of body weight, the second dose was 150 mg, and the third dose was 200 mg.

What was the method of administering the treatments to the mice?

The treatments, including Paracetamol and rambutan seed extract, were administered orally using an oral sonde (tube), while the DPT vaccine was injected intramuscularly.

What was the process for the analgesic test?

For the analgesic test, the mice were first weighed, then treated with different substances (NaCl, Paracetamol, or rambutan extract). After a 15-minute wait, acetic acid (1%) was used to irritate the stomach lining of the mice, and their movements were monitored over 60 minutes.

What specific data were collected during the experiment?

Data collected during the experiment included the rectal temperatures of the mice at specified time intervals and the number of writhing movements observed in the analgesic test. The percentage of pain relief (protection) was also calculated based on these observations.