Pharmacodynamic Screening of Analgesic and Anti Inflammatory Effects revisi 4

Summary

This video demonstrates the practical application of pharmacodynamic testing, focusing on analgesic and anti-inflammatory effects using animal models. Three groups of mice are subjected to different treatments, including acetic acid, meloxicam, and codeine, to induce pain and inflammation. Methods such as the thermal withdrawal hotplate test and edema induction are employed. The video also explores the effects of these treatments on pain relief and inflammation, ultimately evaluating which substances exhibit the strongest analgesic and anti-inflammatory properties. The presentation concludes with an invitation for further exploration of these drug effects in practical settings.

Takeaways

• 😀 The video introduces a pharmacodynamic test to students, focusing on analgesic and anti-inflammatory effects using animal testing.
• 😀 Mice (Mus musculus) are divided into three groups, each receiving a different treatment for testing.
• 😀 The substances prepared for testing include 0.6% acetic acid (injected intraperitoneally) as a pain inducer, meloxicam (oral), and codeine (oral).
• 😀 CMC suspension is used as a control substance in the experiment.
• 😀 The video demonstrates an analgesic test using a hotplate method, where the substances' effects on pain response are observed.
• 😀 The analgesic substances tested include meloxicam, protein, and an unknown compound (mdna).
• 😀 The anti-inflammatory test is also demonstrated using the paw edema method, which involves inducing inflammation in the animals' paws.
• 😀 For inducing inflammation, a compound called 'conflict root Advan' is used to trigger swelling in the animal's paws.
• 😀 Three compounds are tested for their potential analgesic and anti-inflammatory effects: mdna, protein, and meloxicam.
• 😀 At the conclusion of the experiment, the video highlights the compounds' efficacy, pointing out which ones show potential as analgesics or anti-inflammatory agents.

Q & A

What was the primary objective of the experiment described in the transcript?

The primary objective of the experiment was to test the analgesic (pain-relieving) and anti-inflammatory effects of different substances using laboratory mice.

What substances were used in the experiment, and how were they administered?

The substances used were acetic acid (0.6%), meloxicam, and codeine. Acetic acid was administered intraperitoneally, while meloxicam and codeine were given orally.

What method was used to test the analgesic effect of the substances?

The analgesic effect was tested using the thermal withdrawal hotplate method.

What substance was used to induce inflammation in the mice?

Carrageenan was used to induce inflammation in the mice for testing anti-inflammatory effects.

How was the anti-inflammatory effect measured in this experiment?

The anti-inflammatory effect was measured by observing paw edema (swelling) in the mice after carrageenan-induced inflammation.

How were the mice divided for the experiment?

The mice were divided into three groups, each group receiving a different treatment: acetic acid, meloxicam, or codeine.

What is the significance of using a control group in this type of experiment?

The control group, which was given a suspending agent (CMC), is important for comparing the effects of the substances being tested to a baseline, ensuring that observed effects are due to the treatments rather than external factors.

What conclusion did the experiment draw about the substances tested?

The experiment concluded which substances had the most significant analgesic and anti-inflammatory effects. It also identified which substances had minimal or no effect in these areas.

What does the term 'thermal withdrawal hotplate' refer to in the context of this experiment?

The thermal withdrawal hotplate is a method used to measure the pain response of mice by exposing them to a heated surface and observing how long it takes for them to withdraw their paws, indicating the level of analgesia.

Why is it important to include both analgesic and anti-inflammatory tests in pharmacological studies?

Including both tests allows researchers to evaluate the dual action of a substance on pain relief (analgesia) and inflammation, providing a more comprehensive understanding of its therapeutic potential.