PEMERIKSAAN UREUM DARAH (Non Protein Nitrogen)

Summary

This video script details the step-by-step process of conducting a chemical analysis, specifically focusing on serum separation and urea testing. The process involves centrifuging blood samples, separating serum, and preparing reagents for urea testing. It emphasizes the importance of proper identification of samples to avoid mix-ups and careful handling of reagents. The video also covers the operation of lab equipment, checking reagent levels, and performing the actual test while ensuring quality control through regular system checks. Overall, it showcases an essential laboratory procedure for accurate medical testing.

Takeaways

• 😀 The video showcases a process by a chemistry group in a clinic setting.
• 😀 The procedure starts with the preparation of blood samples and their separation into layers after centrifugation.
• 😀 The blood is separated into three layers: clot, buffy coat, and serum.
• 😀 Serum is carefully collected into a simple cup to avoid contamination or mixing with other patient samples.
• 😀 It's essential to label the samples to prevent mix-ups with other patients' samples.
• 😀 The video emphasizes checking reagents before use, ensuring there is enough for the test.
• 😀 Urea reagent is specifically used and needs to be checked for expiration before use.
• 😀 The reagent bottle is placed back correctly after refilling to maintain the integrity of the setup.
• 😀 The machine used for the procedure requires specific control settings to perform the test correctly.
• 😀 Patient identity is recorded through a unique code, ensuring results are correctly linked to the correct patient.
• 😀 After setting up the machine and the necessary checks, the test results are processed and outputted.

Q & A

What is the first step in preparing the blood sample in the process?

The first step involves centrifuging the blood sample at 3600 RPM for 15 minutes.

What are the three layers formed after the centrifugation process?

The three layers formed are the blood clot, buffy coat, and serum.

What is the next step after centrifuging the blood sample?

After centrifugation, the serum is separated into a simple cup (Cup Simple).

What materials are required to prepare the sample for testing?

The materials required include a micropipette, a Cup Simple, and blue tips. Additionally, patient identification must be added to prevent mix-ups.

How should the reagent for urea testing be prepared?

The urea reagent must be checked for adequacy and expiration before being filled into the reagent compartment.

What should you check before using the urea reagent?

Before using the urea reagent, you must check whether the reagent is sufficient and ensure it has not expired.

What is the process of adding the urea reagent to the machine?

The urea reagent is added into the compartment labeled 'urea R1'. It should be filled to the appropriate level, but not overfilled.

What is the significance of checking the expiration date of the urea reagent?

Checking the expiration date ensures that the reagent is effective and has not lost its potency for accurate test results.

What happens after the urea reagent is properly added to the machine?

Once the urea reagent is added and the compartment is resealed, the system prompts for the patient's identity, and the test can begin.

How is the test order processed and how is the patient's identity confirmed?

The test order is input by selecting the appropriate position on the machine. The patient's identity is confirmed using a unique number or code provided in the system.