Pemeriksaan Protein Total (Metode Biuret) Kelompok 1 Kelas A

Summary

This video provides a comprehensive explanation of the total protein test, which measures the protein concentration in serum. It uses the Biuret method, where copper ions react with proteins to form a violet complex, the absorbance of which correlates with protein concentration. The process includes blood collection, serum preparation, and incubation with reagents. The results are measured using a photometer, with normal protein levels ranging from 60-83 grams per liter. Common errors include improper pipetting, reagent quality issues, and using outdated standards. The video aims to educate viewers on conducting and interpreting the total protein test accurately.

Takeaways

• 😀 The total protein test measures the overall protein concentration in serum, including albumin and globulins.
• 😀 The test method used is the Biuret method, where copper ions react with proteins in an alkaline solution to form a purple complex.
• 😀 The test aims to determine the total protein concentration in a serum sample.
• 😀 Blood is collected into a vacuum tube, homogenized, and centrifuged to separate the serum.
• 😀 Reagents and standards are prepared to ensure accurate measurements of the protein concentration.
• 😀 The serum sample is incubated with the Biuret reagent for 10 minutes at room temperature before measuring absorbance.
• 😀 Absorbance is measured at a wavelength of 545 nm using a photometer, which is directly proportional to the protein concentration.
• 😀 A blank, standard, and sample are prepared and measured to calculate the total protein concentration.
• 😀 Normal protein concentration in serum is between 60-83 grams per liter.
• 😀 Errors in the test could arise from improper pipetting, issues during photometric measurement, poor reagent quality, or using outdated standards.
• 😀 The test is crucial for assessing the overall protein status in serum, which is vital for diagnosing various medical conditions.

Q & A

What is the total protein test?

The total protein test measures the overall amount of protein in a blood serum sample, which includes 60% albumin and the rest being globulin.

What is the purpose of the total protein test?

The purpose of the total protein test is to determine the protein concentration in a blood serum sample.

What method is used to perform the total protein test?

The Biuret method is used for the total protein test. This method involves the reaction of copper ions with proteins in an alkaline solution, forming a purple complex, the absorbance of which is proportional to the protein concentration.

What equipment and materials are required for the sample collection?

The materials needed for sample collection include a tourniquet, a 5cc vacuum tube with a yellow cap, cotton, alcohol swabs, a centrifuge, and a test tube.

How is the blood sample processed before the protein test?

After blood is collected in a vacuum tube, it is homogenized and centrifuged for 5-10 minutes at 3000 rpm. The serum is then separated using a micropipette and placed into a test tube.

What are the main tools required for performing the protein test?

The main tools include a photometer, micropipettes, vortex mixer, test tubes, and a protein total analyzer.

How are the reagents prepared for the total protein test?

For the test, reagents are prepared by mixing specific amounts of reagent A and distilled water (for blank), protein standard (for the standard tube), and serum sample (for the sample tube). All of these are then incubated for 10 minutes at room temperature.

What is the incubation time for the total protein test?

The incubation time for the total protein test is 10 minutes at room temperature.

How is the absorbance of the sample measured?

After incubation, the absorbance of the sample is measured using a photometer set at a wavelength of 545 nm.

What factors can cause abnormal results in the protein total test?

Factors that can cause abnormal results include improper pipetting, incomplete aspiration of the solution into the photometer, poor quality reagents, and using outdated protein standard values.