Cara Kerja Pemeriksaan CRP metode Kualitatif

Summary

This video provides a detailed explanation of the CRP (C-reactive protein) test, its purpose, and the procedure involved. The test is used to detect and monitor inflammation in both acute and chronic conditions. The process includes blood collection, centrifugation, and preparation of a black slide. The serum is applied to different wells on the slide, with controls added for accuracy. Latex reagents are mixed, and the slide is rotated or shaken to observe for agglutination. The presence of agglutination indicates a positive result, while its absence shows a negative result. The tutorial concludes with an appreciation for the procedure's importance.

Takeaways

• 😀 The CRP test helps detect or monitor inflammation in acute or chronic conditions.
• 😀 Tools and materials required for the CRP test include a micropipette, serum, tips, Regen latex, tissue, black slide, stirring rod, and a rotator.
• 😀 The first step in the CRP test involves drawing blood from a vein.
• 😀 After collecting the blood, it is centrifuged at 3000 revolutions per minute for 5 to 15 minutes.
• 😀 Prepare a black slide and drop 50 microliters of serum into the first hole.
• 😀 In the second hole, add one drop of positive control serum, and in the third hole, add one drop of negative control serum.
• 😀 For both the positive and negative controls, add one drop of latex ligand, then stir the mixture with a stirring rod.
• 😀 The samples are then rotated for 1 to 2 minutes at a speed of 100 revolutions per minute, or they can be shaken manually if a rotator is unavailable.
• 😀 Observe for agglutination. A positive result will show agglutination, while a negative result will show no agglutination.
• 😀 This test is used to evaluate the presence of inflammation and can help track the progress of acute or chronic inflammatory conditions.

Q & A

What is the purpose of a CRP test?

The purpose of a CRP (C-reactive protein) test is to detect or monitor inflammation in both acute and chronic conditions.

What are the essential materials needed for the CRP test?

The essential materials required for the CRP test include a micro-pipette, serum, tips, reagents (latex), tissue, a black slide, a stirring stick, and a rotator.

How is the blood sample prepared for the CRP test?

The blood sample is taken from a vein and then centrifuged at 3000 RPM for 5 to 15 minutes to separate the serum.

How is the serum applied on the black slide during the test?

50 microliters of serum are placed on the black slide in the first well, followed by a drop of positive control solution in the second well, and a drop of negative control solution in the third well.

What is added to the serum and control solutions during the test?

A latex reagent is added to both the serum and the control solutions (positive and negative).

How is the mixture processed after the reagents are added?

The mixtures are stirred with a stirring stick or rotated using a rotator for 1 to 2 minutes at 100 RPM to ensure proper mixing.

What happens if a rotator is not available for the test?

If a rotator is unavailable, the slide can be manually shaken to mix the reagents and serum.

What is observed to determine the result of the CRP test?

The presence of agglutination is observed to determine the result. If agglutination occurs, the result is positive for inflammation. If no agglutination occurs, the result is negative.

What does agglutination indicate in the CRP test?

Agglutination indicates that the CRP test result is positive, suggesting the presence of inflammation.

How long does the centrifugation step take during the CRP test?

The centrifugation step should be performed at 3000 RPM for 5 to 15 minutes to properly separate the serum from the blood cells.