Cara Kerja Pemeriksaan RF

Summary

This video explains the procedure for conducting a Rheumatoid Factor (RF) test. The process begins with blood collection via venipuncture, followed by centrifugation to separate the serum. A black slide is prepared, and 50 microliters of serum is added to the first well, with positive and negative controls placed in the second and third wells, respectively. Latex reagent is then added to each well, mixed, and agitated. The reaction is observed for agglutination, with a positive result indicating the presence of RF. This test helps assess the level of RF in the blood, which is important for diagnosing conditions like rheumatoid arthritis.

Takeaways

• 😀 The purpose of the Nerf test is to determine the amount of RF (Rheumatoid Factor) in the blood.
• 😀 The procedure involves using several materials, such as a micropipette, serum, tip, and various other items.
• 😀 Blood is first collected via venipuncture and then centrifuged at 3000 revolutions per minute for 5 to 15 minutes.
• 😀 A black slide is prepared, and 50 microliters of serum is placed in the first well.
• 😀 A drop of positive control is added to the second well, and a drop of negative control is added to the third well.
• 😀 Positive and negative control serums are treated with one drop of latex reagent.
• 😀 The mixtures are stirred using a stirring rod to ensure homogeneity.
• 😀 The slide is then rotated or shaken gently by hand to allow the reactions to occur.
• 😀 The presence or absence of agglutination is observed as the final result of the test.
• 😀 If agglutination occurs, the test result is positive for RF, and if no agglutination occurs, the result is negative.

Q & A

What is the purpose of the RF test described in the script?

The RF test aims to determine the level of rheumatoid factor (RF) present in the blood.

What materials are required for performing the RF test?

The materials needed include a micropipette, serum, tips, latex reagent, tissue, black slide, stirring rod, and a rotator.

How is the blood sample processed before starting the RF test?

The blood is drawn from a vein and then centrifuged at 3000 RPM for 5 to 15 minutes to separate the serum.

What is the first step in setting up the slide for testing?

The first step is to place 50 microliters of serum onto the first hole of the black slide.

How are the positive and negative control samples handled during the test?

One drop of positive control serum is placed in the second hole, and one drop of negative control serum is placed in the third hole on the slide.

What is added to each serum drop after placing them on the slide?

One drop of latex reagent is added to each serum drop (serum, positive control, and negative control).

How are the serum and reagent mixed?

The serum and latex reagent are mixed using a stirring rod until the mixture is homogeneous.

What is the next step after mixing the serum and latex reagent?

After mixing, the slide is either rotated using a rotator or shaken gently by hand.

How can the results of the test be interpreted?

If agglutination occurs, the result is positive. If there is no agglutination, the result is negative.

What does the presence of agglutination indicate in the RF test?

The presence of agglutination indicates a positive result, meaning rheumatoid factor is present in the blood.