Mechanism of Ripening

Students' Channel
3 Jan 202405:18

Summary

TLDRThe video script delves into the intricate process of fruit ripening, a natural and essential developmental stage for plants. It highlights the three phases of ripening, emphasizing its coordination with seed maturation and the role of thousands of genes. The script explains how ripening involves changes in fruit composition, such as starch to sugar conversion, color alteration, and softening. It also discusses external and internal factors influencing ripening, the role of ethylene in promoting fruit ripening and respiration, and the enzymatic processes leading to color and textural changes. The summary underscores the complex interplay of genetic, biochemical, and environmental elements that contribute to the ripening of fruits, affecting their flavor, aroma, and texture.

Takeaways

  • 🍇 Ripening is a natural process that transforms fruits into a state of desirable flavor, color, and texture, which is essential for seed dispersal.
  • 🧬 The ripening process is complex and involves a network of genes and signaling pathways that are unique to plants.
  • 📈 There are three phases of ripening, which are coordinated with the maturation of seeds and regulated by thousands of genes.
  • 🍌 Banana ripening serves as an example, showing changes in color, firmness, and odor as the fruit matures.
  • 🔄 Internal factors such as stage of development, moisture content, and tissue type, as well as external factors like temperature and humidity, affect the ripening process.
  • 🌡 Ethylene plays a crucial role in ripening by promoting its own biosynthesis and increasing respiration, especially in climacteric fruits.
  • 🌿 Chlorophyll degradation during ripening leads to color changes, mainly due to pH changes, oxidation, and the action of chlorophyllase.
  • 🍯 Hydrolysis of starch to sugar is a key biochemical change during ripening, increasing the fruit's sweetness.
  • 🥭 Textural softening occurs due to enzymatic degradation of cell wall components and loss of turgor pressure, affecting the fruit's firmness.
  • 🌬 Production of volatile compounds, such as esters and alcohols, contributes to the aroma and flavor of ripe fruits.
  • 🍏 Changes in organic acids, like malate and citrate, and the action of enzymes can influence the taste and quality of ripe fruits.
  • 🛠 Ethylene regulation techniques, including the use of ionizing radiation and bioregulators, can be employed to manage the ripening process.

Q & A

  • What is the definition of ripening in the context of fruits?

    -Ripening is the process by which fruits attain their desirable flavor, quality, color, palatable nature, and other textural properties.

  • What is unique about the ripening process in plants?

    -Ripening is unique to plants as it involves the development into a fruit, requiring an intricate web of interconnected genes and signaling channels.

  • How many phases does the ripening process consist of?

    -The ripening process consists of three separate phases.

  • What does the ripening process coincide with in fruits?

    -The ripening process coincides with seed maturation in fruits.

  • How many genes are involved in regulating the ripening process?

    -The ripening process is regulated by thousands of genes.

  • What changes are associated with fruit ripening?

    -Fruit ripening is associated with changes in composition, such as conversion of starch to sugar, change in color, firmness, odor, and the release of volatiles.

  • What are the internal factors affecting the ripening of fruits?

    -Internal factors affecting ripening include stage of development, moisture content, peel thickness, type of tissue, and respiration.

  • What are the external factors influencing fruit ripening?

    -External factors influencing ripening are temperature, radiation, air humidity, mechanical damage, and the concentration of O2 and CO2.

  • What role does ethylene play in the ripening process?

    -Ethylene promotes its own biosynthesis, increases respiration, and is involved in the climacteric rise in fruits, which is accompanied by an increased oxygen uptake.

  • What happens to chlorophyll during the ripening process?

    -During ripening, chlorophyll undergoes degradation due to pH changes, oxidation systems, and the action of chlorophyllase, leading to a color change from bright green to dull olive green.

  • How does the texture of fruit change during ripening?

    -Texture softening during ripening is the result of enzymatic degradation of structural and storage polysaccharides, leading to reduced cellular rigidity and loss of turgor pressure.

  • What are the major volatile compounds found in fruits?

    -Major volatile compounds in fruits are esters of aliphatic alcohols and short-chain fatty acids, contributing to the fruit's aroma.

  • What contributes to the taste of fruits during ripening?

    -Taste is provided by many nonvolatile components including sugars and acids present in fruits, as well as short-chain unsaturated aldehydes and alcohols and esters.

  • What changes occur in organic acids during fruit ripening?

    -Changes in organic acids like malate and citrate occur during fruit ripening, which can affect the taste, along with the possible influence of phenolic compounds and tannins.

  • How does the onset of ripening affect fruit properties?

    -The onset of ripening increases membrane permeability, reduces translocation from the leaves, and leads to the conversion of starch to sugar and an increase in fruit volume, diluting the acids.

  • What are some methods for regulating the ripening process?

    -Regulation of ripening can be achieved through the application of ethylene, control of O2 and CO2 concentrations, calcium application, using ionizing radiation, and bioregulators.

Outlines

00:00

🍌 Fruit Ripening Process and Mechanisms

This paragraph delves into the natural process of fruit ripening, which is essential for fruits to develop their desirable characteristics such as flavor, color, and texture. It highlights that ripening is a complex, genetically regulated process involving thousands of genes and three distinct phases that align with seed maturation. The paragraph explains the biochemical changes that occur during ripening, including the conversion of starch to sugar, softening of the fruit's texture due to enzymatic degradation of cell wall components, and the production of volatile compounds that contribute to the fruit's aroma. It also discusses the role of ethylene, a hormone that promotes ripening by increasing respiration and its own biosynthesis, leading to irreversible changes in the fruit. The summary underscores the importance of internal factors like development stage, moisture content, and tissue type, as well as external factors such as temperature and mechanical damage, in influencing the ripening process.

05:00

🔬 Regulation of Fruit Ripening

The second paragraph focuses on the regulation of the fruit ripening process. It mentions the use of ethylene, a key hormone in ripening, and how its regulation can impact the ripening process. The paragraph also discusses various methods to control ripening, such as the application of calcium, using ionizing radiation, and employing bioregulators. These techniques can help manage the ripening process to ensure fruits maintain their quality and are ready for consumption or sale at the desired time. The summary emphasizes the importance of managing ethylene levels and other factors to optimize the ripening process for different fruits, taking into account their unique requirements and the desired outcomes for consumers.

Mindmap

Keywords

💡Ripening

Ripening is the natural process by which fruits develop their full flavor, color, and texture. It is a complex and highly coordinated developmental process that aligns with seed maturation. In the context of the video, ripening is depicted as an intricate web of genes and signaling pathways that lead to the transformation of a fruit into a palatable and desirable product. For example, the video mentions that ripening involves changes such as starch to sugar conversion and softening of the fruit's texture.

💡Chlorophyll

Chlorophyll is a green pigment found in plants, essential for photosynthesis. During the ripening process, as described in the video, chlorophyll degradation occurs, leading to a change in the fruit's color. This degradation is primarily due to pH changes, oxidation systems, and the action of chlorophyllase, which results in the replacement of the magnesium atom in chlorophyll with a hydrogen atom under acidic conditions, causing a color change from bright green to dull olive green.

💡Starch Hydrolysis

Starch hydrolysis refers to the breakdown of starch into simpler sugars. The video script explains that this process is a key part of fruit ripening, as it increases the concentration of sugars within the fruit, contributing to its sweetness. In climacteric fruits, the starch content generally increases during development but decreases during storage due to its use for metabolic purposes.

💡Textural Softening

Textural softening is the process by which a fruit's texture becomes less firm as it ripens. The video describes this as being controlled by the enzymatic degradation of structural and storage polysaccharides, which leads to a breakdown of cell wall adhesion and a reduction in cellular rigidity. This softening is a desirable quality in ripe fruits, making them more palatable.

💡Ethylene

Ethylene is a plant hormone that plays a crucial role in the ripening process. The video script highlights its function as a promoter of fruit ripening, including its own biosynthesis and the increase in respiration. Ethylene is particularly important in climacteric fruits, where it is associated with an increase in oxygen uptake and is largely predetermined by the fruit's genetics. It is also mentioned that external ethylene can enhance ripening.

💡Volatile Compounds

Volatile compounds are substances that easily evaporate and contribute to the aroma of fruits. The video script notes that these compounds, such as esters of aliphatic alcohols and short-chain fatty acids, originate from proteins, carbohydrates, lipids, and vitamins within the fruit. They are significant contributors to the fruit's aroma and are produced during the ripening process.

💡Color Changes

Color changes are a visual indicator of the ripening process. The video script discusses the transformation of chlorophyll into colorless products and the formation of anthocyanin in the vacuole and epidermal layer, which contribute to the change in a fruit's color from green to more ripe hues. These color changes are a result of various biochemical reactions, such as chlorophyll degradation and the masking effect of chlorophyll.

💡Pigments

Pigments are substances that give color to living organisms. In the context of the video, pigments such as anthocyanin are mentioned as they play a role in the color changes that occur during fruit ripening. The formation of these pigments in the vacuole and epidermal layer contributes to the visual appeal of ripe fruits.

💡Cell Wall Degradation

Cell wall degradation is the breakdown of the structural components of a plant cell's wall. The video script explains that this process is part of textural softening during fruit ripening. The enzymatic degradation of structural polysaccharides leads to a loss of cell wall mechanical strength and cell-to-cell adhesion, resulting in a softer fruit texture.

💡Respiration

Respiration is a metabolic process in which organic molecules are broken down to produce energy. The video script mentions that during the ripening process, respiration increases, particularly in response to ethylene. This increase in respiration is associated with the climacteric peak, a period of intense metabolic activity that coincides with fruit ripening.

💡Bioregulators

Bioregulators are substances that regulate biological processes. In the video script, the use of bioregulators such as calcium application and ionizing radiation is mentioned as a means to regulate the ripening process. These substances can influence the rate and quality of fruit ripening, affecting factors such as texture, color, and flavor.

Highlights

Ripening is a natural process by which fruits attain desirable flavor, color, and textural properties.

The ripening process requires a complex interplay of genes and signaling pathways.

Fruit ripening is a coordinated developmental process that coincides with seed maturation.

Thousands of genes regulate the ripening process, controlling softening, sugar accumulation, and volatile release.

Banana ripening color chart illustrates the changes associated with ripening.

Ripening involves changes in fruit composition, including starch to sugar conversion and color changes.

Internal factors such as stage of development and moisture content affect the ripening process.

External factors like temperature and air humidity play a role in ripening.

Ethylene is crucial in ripening as it promotes its own biosynthesis and increases respiration.

Chlorophyll degradation during ripening is due to pH changes and oxidation systems.

Hydrolysis of starch to sugar increases sugar concentration in fruits.

Textural softening is a result of enzymatic degradation of structural polysaccharides.

Production of volatiles such as esters and aliphatic alcohols contributes to fruit aroma.

Changes in organic acids and phenolic compounds can affect the taste of ripening fruits.

Increase in fruit volume during ripening can lead to dilution of acids.

Ethylene regulation and the application of calcium and ionizing radiation are methods to control ripening.

Ripening involves a complex interaction of internal and external factors with genetic predispositions.

Transcripts

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mechanisms of

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ripening mechanisms a natural or

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established process by which something

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takes place or is brought about ripening

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is the process by which fruits attain

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their desirable flavor quality color

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palatable nature and other textural

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properties what is

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ripening unique to plants the process of

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developing into a fruit an intricate web

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of interconnected genes and signaling

play00:30

channels is needed for it there are

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three separate phases that freshly fruit

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houses

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through ripening is a highly coordinated

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developmental process that coincides

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with seed maturation the ripening

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process is regulated by thousands of

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genes that control Progressive softening

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and or

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orifices accumulation of sugars acids

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pigments and release of volatiles banana

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ripening color chart ripening is

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associated with change in composition

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conversion of starch to sugar change in

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color change in firmness odor or smell

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factors affecting

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ripening internal Factor one stage of

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development two moisture content three

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peel thickness four type of tissue five

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volatil six transpiration s respiration

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external Factor one temperature two

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radiation three air humidity four

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mechanical damage five O2 and Co to

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concentration six growth

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substances role of ethylene in

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ripening promotes its own biosynthesis

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external ethylene hen climacteric

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accompanied by an increased oxygen

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uptake this process is not reversed

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external ethylene increase respiration

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ethylene production is largely

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predetermined in both time and amount by

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genetics of the

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fruit changes during

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ripening chlorophyll degradation

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hydrolysis of starch to sugar textural

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softening cell wall degradation

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production of volatiles compound changes

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in amino acids and protein involvement

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of enzyme color changes

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the chlorophyll degradation is mainly

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due to pH changes oxidation systems

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chlorophyllase formation of katene in

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plastid formation of anthocyanin in vacu

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and in epidermal layer masking effect of

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chlorophyll degradation of chlorophyll

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into colorless

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product mechanism of chlorophyll

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degradation replacement of magnesium

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atom in the chlorophyll by hydrogen atom

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under acidic condition with the

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formation of fopen fopen formation a

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color change from bright green to dull

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olive green hydrolysis of chlorophyll to

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chlorophyllase followed by a replacement

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of magnesium atom with

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hydrogen hydrolysis of starch to

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sugar increase in the concentration of

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sugars either by hydrolysis of starch

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within the fruit or by continued import

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of sugars from other part of the plant

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in climacteric fruits the starch content

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generally increases during development

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but circus decrease during storage due

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to its usage for

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metabolic textural

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softening texture is controlled by the

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wall to wall adhesion of cells breakdown

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of starch breakdown of pectic substance

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reduced cellular rigidity softening it

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is the result of enzymatic degradation

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of structural as well as storage

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polysaccharides ripening probably

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depends on the species change in cell

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wall composition especially cell wall

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mechanical strength and cellto cell

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adhesion loss of turer pressure

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degradation of starch production of

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Aroma

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volatiles Esters of aliphatic alcohols

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and short chain fatty acids dot dot in

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fruits major volatile compounds are

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Isomil acetate aldah and turpenoid

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compounds volatiles originate from

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proteins carbohydrates lipids and

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vitamins taste is provided by many

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nonvolatile components including sugars

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and acids present in fruits short chain

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unsaturated aldah and alcohol and Esters

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are important contributors to Aroma

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changes in organic acids malate and

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citrate are more common among fruits

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also phenolic compounds and Tannis may

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affect the taste onset of ripening

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increase membrane permeability reduction

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in translocation from the leaves

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conversion of starch to sugar acids are

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used in respiration

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increase in volume of fruit leading to

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dilution of acids regulation in

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ripening ethylene regulation regulation

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of o2 and CO2 calcium application using

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ionizing radiation bioregulators

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Related Tags
Fruit RipeningEthylene RoleChlorophyll DegradationStarch HydrolysisTextural ChangesFlavor DevelopmentRespiration RatePigment FormationVolatile CompoundsBioregulators