PRAKTIKUM ENZIM KATALASE pada HATI AYAM (BIOLOGI KLS XII)
Summary
TLDRIn this educational video, the instructor guides students through a practical experiment on the enzyme catalase, focusing on factors affecting its activity. Using blended chicken liver, the experiment tests the enzyme's reaction to various pH levels and temperatures by observing the breakdown of hydrogen peroxide into water and oxygen. The presence of oxygen is confirmed by a glowing ember. The video highlights the enzyme's efficiency in neutral conditions and its inactivity under acidic conditions or high temperatures, emphasizing the importance of liver function in detoxifying the body.
Takeaways
- 🧪 This practical session is about the enzyme catalase, part of the metabolic process discussed in Chapter 2.
- 🦴 The experiment will test factors affecting catalase activity found in the liver.
- 🍗 Materials used include blended chicken liver, distilled water, HCl, H2O2, test tubes, test tube clamps, dropper pipettes, a Bunsen burner, and wooden sticks.
- 🌡️ The first test involves adding 20 drops of chicken liver solution to a neutral pH solution at room temperature.
- 🧴 The second test uses a basic pH solution by adding 5 drops of NaOH, mixed with liver solution.
- 🥽 The third test involves an acidic pH solution by adding 5 drops of HCl to the liver solution.
- 🔥 The fourth test heats the liver solution to high temperatures until boiling.
- 🫧 H2O2 is added to each test tube to observe the reaction, indicated by bubble formation.
- 🧯 Oxygen production is tested by seeing if a glowing stick ignites in the presence of bubbles.
- 💡 The results show catalase activity: effective in neutral and basic pH, but not in acidic pH or high temperatures.
Q & A
What is the main topic of the practical lesson discussed in the script?
-The main topic of the practical lesson is testing the factors that affect the activity of the enzyme catalase.
What are the primary materials used in the experiment?
-The primary materials used in the experiment include blended chicken liver, distilled water, HCl, H2O2, test tubes, test tube holders, pipettes, a Bunsen burner, and wooden splints.
What is the purpose of blending the chicken liver in the experiment?
-Blending the chicken liver is done to obtain a homogenized mixture that contains the enzyme catalase.
What role does H2O2 (hydrogen peroxide) play in the experiment?
-H2O2 is used to test the activity of the catalase enzyme. Catalase breaks down H2O2 into water and oxygen.
How is the effect of pH on catalase activity tested?
-The effect of pH on catalase activity is tested by adding NaOH to create a basic solution and HCl to create an acidic solution, and then observing the enzyme activity in these different pH conditions.
What observation indicates that oxygen is being produced in the reaction?
-The production of bubbles in the reaction indicates that oxygen is being produced, and this can be further confirmed by a glowing splint test, where the splint reignites in the presence of oxygen.
How does temperature affect the activity of catalase in this experiment?
-The experiment tests the effect of temperature by heating one of the test tubes containing the liver mixture. Heating denatures the enzyme, preventing it from breaking down H2O2, which is indicated by the absence of bubbles.
What conclusion can be drawn about the enzyme catalase's activity at different pH levels?
-Catalase is active at a neutral pH but becomes inactive in highly acidic or basic conditions.
What is the practical implication of catalase activity in the human body mentioned in the script?
-The practical implication is that catalase helps detoxify hydrogen peroxide, a harmful byproduct of metabolism, by converting it into water and oxygen.
What does the script suggest about the stability of catalase at high temperatures?
-The script suggests that catalase becomes denatured and inactive at high temperatures, as indicated by the lack of bubbles when the heated liver mixture is tested with H2O2.
Outlines
🔬 Introduction to Catalase Enzyme Experiment
The video introduces a practical experiment on catalase enzyme, part of a metabolic chapter. The experiment aims to investigate factors affecting catalase activity in chicken liver. The materials used include blended chicken liver, hydrochloric acid (HCl), hydrogen peroxide (H2O2), basic solution (KOH), test tubes, a Bunsen burner, and other laboratory tools. The experiment tests catalase activity under different pH conditions and temperatures, using a neutral pH solution, a basic solution with KOH, and an acidic solution with HCl. The hydrogen peroxide is added to test tubes containing the catalase enzyme from chicken liver, and the formation of oxygen bubbles is observed.
💨 Observing Oxygen Production in Catalase Activity
The second paragraph describes the observation of oxygen production by the catalase enzyme. The experiment verifies oxygen release by relighting a glowing splint, indicating the presence of oxygen. The production of bubbles shows that catalase converts hydrogen peroxide into water and oxygen, detoxifying the harmful substance. The experiment further examines the enzyme's activity in different pH environments. It reveals that catalase is active in neutral and basic conditions, producing bubbles, but does not work in acidic conditions, as no bubbles or oxygen production is observed. Additionally, the experiment explores the impact of temperature, showing that heating denatures the enzyme, rendering it inactive in converting hydrogen peroxide.
Mindmap
Keywords
💡Enzyme
💡Catalase
💡Hydrogen Peroxide (H2O2)
💡pH
💡Temperature
💡Denaturation
💡Metabolism
💡Substrate
💡Reaction Rate
💡Bunsen Burner
Highlights
Introduction to the practical session on enzyme catalase, part of Chapter 2 on metabolism.
Overview of the materials needed: blended chicken liver, distilled water, HCl, H2O2, test tubes, test tube holders, dropper pipettes, Bunsen burner, and splints.
Demonstration of neutral pH reaction at room temperature using 1 ml (20 drops) of the chicken liver solution.
Experiment to observe the effect of a basic pH by adding 5 drops of KOH to the chicken liver solution.
Experiment to observe the effect of an acidic pH by adding 5 drops of HCl to the chicken liver solution.
Heating a test tube to observe the effect of high temperature on the enzyme catalase activity.
Addition of H2O2 to the test tubes containing the chicken liver solution to observe the formation of oxygen bubbles.
Verification of oxygen production by introducing a glowing splint, which reignites in the presence of oxygen.
Confirmation that enzyme catalase breaks down H2O2 into water and oxygen, reducing its toxicity.
Observation that the enzyme catalase functions at a neutral pH as indicated by bubble formation.
Observation that the enzyme catalase also functions in a basic environment, as shown by bubble formation.
Failure of the enzyme catalase to function in an acidic environment, as indicated by the absence of bubbles.
Confirmation that high temperatures denature the enzyme catalase, preventing it from breaking down H2O2.
Conclusion emphasizing the importance of liver enzymes in detoxifying the body by breaking down H2O2.
Encouragement to fill out the provided data forms to complete the practical session.
Transcripts
hai sahabat alaikum warahmatullah
wabarakatuh anak-anakku sekalian kali
ini kita akan praktikum tentang enzim
katalase masuk ke dalam bab 2 di bab
metabolik nah yang akan kita lakukan
hari ini adalah menguji faktor-faktor
yang mempengaruhi kerja enzim katalase
yang ada di dalam hati ia yakin oke apa
saja bahan-bahannya
ayo kita lihat di atas menjadi ini sudah
kesekian ada hati ayam yang sudah
diblender dan sudah diberikan sedikit
lahir kemudian disediakan aku ha
kemudian disediakan hcl h2o 2 kemudian
dengan alat beberapa tabung reaksi
penjepit tabung reaksi dan pipet tetes
kemudian ada pembakar bunsen kemudian
ada lidi membangkakng sekarang kita akan
memasukkan sekitar satu milih yaitu 20
tetes dari larutan hati ayam akan
ingatkan aku untuk yang ph netral dengan
suhu ruang kemudian satu yang kedua klub
yang sudah ditambahkan dengan ph basa
yaitu dalam hal ini kokoh maka kita akan
tambahkan lima tetes kuhd dalamnya dan
kita kocok kemudian kita masukkan hcl
juga sama sebanyak lima tetes untuk
menunjukkan bahwa campurannya ph asam
kemudian 1tabung lagi akan kita bakal
kita tadi kita
[Musik]
hai hongkong kupu-kupu ini kamu jadi
suhu tinggi dengan kita panaskan mungkin
ih anak tapi jadi ini kita panaskan
dipanaskan gini sampai mendidih hingga
akhirnya protein yang ada di dalam
karena sudah tadi kita isi 4 kabun kita
akan isi h2 o2 h2o ini adalah hidrogen
peroksida yang ada di dalam sel-sel
hasil dari metabolisme nah kita isi sama
sebanyak empat tahun di
curah yang sama sekitar 20 tetes atau 1
miliar ini sudah ada empat buah ya
tabung berisi h2o 2 dan 4 buah tabung
yang berisi enzim katalase dari hanya ya
maafin saya untuk megang apa namanya
menahan oksigen dan h2o yang nanti
berupa gelembung udara yang akan
terbentuk dari peristiwa kita masukkan
h2 o2 ke dalam tekan yang berisi tapi
[Musik]
hai hai
hai kya keh sudah mulai kelihatan disitu
membentuk gelembung terbentuk gelembung
dan sekarang kita akan coba apakah benar
menghasilkan oksigen nah oksigen itu
udah sekali terbakar maka kita akan
lihat dari bara api yang ada di lido ini
menyala ya nah menyala ini artinya bahwa
memang benar di dalam gelembung ini
dihasilkan oksigen h2o 2 ya yang diubah
oleh enzim katalase menjadi h2o dan
oksigen artinya h2o 2 ini bisa diubah
menjadi tidak beracun h2 o2 adalah sisa
metabolisme yang dihasilkan oleh tubuh
kita yang bersifat racun yang dapat
tekan bu sangat lengket-lengket sekarang
kita ingin membuktikan apakah dengan
kondisi lingkungan yang basah enzim ini
tetap masih bisa bekerja kita lihat
sekarang sama h2o 2 kita akan tuang
kedalam larutan yang sudah berisi enzim
katalase dari hati air nilailah mungkin
ternyata memang benar ada gelembung yang
rapat ya bukanya kita bisa lihat ini
juga akan menyebabkan nyala menyalanya
didih yang ada berani cuma ini hal-hal
dur senter bentuk enggak punya bisanya
apinya menyala padahal saya masukkan
adalah barat nah ini menandakan bahwa
enzim katalase yang bekerja pada ph
sekarang lanjut bagaimana dengan ph asam
kita akan lihat dengan ditambahkan hcl
akan terbentuk gelembung juga seperti
pada sampai hanya terang dan ph kita
tamat lutut tetapi percuma ya ditutup
juga tidak ada artinya apa artinya
anjing ini ternyata tidak bisa bekerja
pada ph ke
40 kutersenyum nah sekarang kita akan
lihat apakah faktor sungguh juga
mempengaruhi kerja untuk mengetahui
apakah dengan suhu tinggi maka enzim ini
[Musik]
[Musik]
kayaknya sudah dipanaskan ternyata
[Musik]
dengan dipanaskan enzim katalase yang
ada di hati ayam tidak bisa bekerja
mengubah h2o 2 menjadi air dan oksigen
dalam hal ini h2o 2 berarti masih tetap
dalam pengungkapan
ngomong hero permanen kita harus
bersyukur allah menciptakan hati dalam
tubuh kita satu nyatanya fungsi untuk
demikian mudah-mudahan demonstrasi
praktikum enzim katalase kali ini
bermanfaat silahkan isi datanya sudah
saya kirimkan ke
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