Bagian-Bagian Instrument KCKT (Kromatografi Cair Kinerja Tinggi) - STFI Edukasi

Summary

In this informative video, a pharmacy student from the School of Pharmacy Indonesia presents the components and functionality of the Waters HPLC model 1525. Key elements discussed include the inert mobile phase vessels, dual pumps for liquid mixing, the injector for sample introduction, and the C18 column for component separation. A UV detector identifies the sample components, while data processing occurs on a connected computer, producing chromatograms that illustrate separation. The student emphasizes the importance of laboratory safety, making this a valuable overview for those interested in analytical techniques.

Takeaways

• 😀 The video introduces the HPLC Waters 1525 and its key components.
• 🔍 The mobile phase can hold between 500 mL to 1 L and is made of inert glass.
• 💧 There are two pumps in the system: Pump A and Pump B, each drawing from a different mobile phase.
• 💉 Samples are injected using a glass syringe with a volume of 25 microliters, typically injecting 20 microliters.
• 🔄 The injector has a 'load' position for sample preparation before injection.
• 🧪 The column used is a C18 non-polar type, crucial for separating sample components.
• 📊 Components are detected via UV detectors, with wavelengths ranging from 200 to 400 nm.
• 🚮 After detection, the sample is disposed of in a waste channel.
• 💻 HPLC is connected to a computer for data processing using software that produces chromatograms.
• 🖨️ Results can be printed, showing peak separations on the chromatogram.

Q & A

What is the primary function of the HPLC Waters 1525?

The HPLC Waters 1525 is designed for high-performance liquid chromatography, which is used to separate and analyze components in a sample.

What materials are used for the mobile phase in the HPLC system?

The mobile phase is contained in inert glass vessels to ensure compatibility and avoid reactions with the solvent.

How many mobile phase reservoirs are used in the HPLC 1525, and what are they labeled as?

There are two mobile phase reservoirs in the HPLC 1525, labeled as A and B.

What role do the pumps play in the HPLC system?

The pumps draw the mobile phase from reservoirs A and B, mix them in a predetermined ratio, and then transport the mixture to the injector.

What is the purpose of the injector in the HPLC process?

The injector is used to introduce the sample into the HPLC system, typically using a glass syringe.

What type of column is used in the HPLC process, and what is its characteristic?

The column used is a C18 type, which is nonpolar and helps separate the components within the sample.

How does the detector function in the HPLC system?

The detector identifies the components of the sample by measuring their absorbance at UV wavelengths between 200 and 400 nm.

What happens to the sample after it passes through the detector?

After detection, the sample is disposed of through a waste outlet.

How is the data processed after the sample analysis?

The signals from the detector are processed by a recorder connected to a computer, allowing for immediate data analysis.

What is indicated by the peaks in the chromatogram?

The peaks in the chromatogram represent the separation of components in the sample, allowing for quantitative analysis.