Pemeriksaan kadar Hb dengan metode sahli

Summary

This educational video demonstrates how to measure hemoglobin (HB) levels using the Sahli method. The process involves using a Sahli hemometer, hydrochloric acid, and a blood sample to compare the color change in the solution to a standard. The procedure includes cleaning and preparing equipment, mixing blood with HCl, and adjusting the solution with aquades until the color matches the standard. The results are then read visually to determine the hemoglobin concentration. The video also discusses normal and abnormal hemoglobin levels and the implications for health, such as anemia or dehydration.

Takeaways

• 😀 The Sahli method is used to determine hemoglobin (HB) levels based on the color change when hemoglobin is converted to acid hematin by hydrochloric acid (HCl).
• 😀 A hemometer is used as the standard tool to compare the color of the solution in the reaction tube with the standard color of hemoglobin.
• 😀 The main tools required for this experiment are the Sahli hemometer, pipettes, mixing stick, HCl solution (0.1 N), distilled water (aquades), and blood samples.
• 😀 Before taking blood samples, ensure all equipment is properly cleaned with alcohol and tissue to avoid contamination.
• 😀 The blood sample is taken using a Sahli pipette, which is connected to a rubber tube. The pipette should be filled with 0.02 ml of blood before being added to the reaction tube.
• 😀 After the blood is added to the reaction tube containing HCl, mix the contents gently using a stirring rod to ensure the solution is homogeneous.
• 😀 The solution is incubated for 3 minutes at room temperature before distilled water (aquades) is added slowly while stirring to match the color of the solution to the standard.
• 😀 The color of the solution in the reaction tube is compared to the standard hemometer by viewing it at eye level to ensure proper alignment with the color scale.
• 😀 The result of the hemoglobin concentration is determined based on the final color match and is read from the hemometer scale, where each mark represents 0.2 grams of hemoglobin per deciliter.
• 😀 Normal hemoglobin levels for women are between 12-16 g/dL and for men between 14-18 g/dL. Low hemoglobin levels may indicate anemia, while high levels may be linked to dehydration, COPD, or heart failure.

Q & A

What is the principle behind the Sahli method for determining hemoglobin concentration?

The principle of the Sahli method involves measuring the hemoglobin concentration based on the color change that occurs when hemoglobin is converted into acid hematin by 0.1 N hydrochloric acid (HCl). The resulting color is then visually compared with a standard on a hemometer.

What are the main materials needed for the Sahli method in hemoglobin determination?

The key materials required for the Sahli method include a Sahli hemometer, a pipette, a stirring rod, a blood sample, 0.1 N HCl, aquades (distilled water), and a Sahli pipette along with a hose for drawing the blood.

How do you prepare the hemometer for the hemoglobin measurement?

To prepare the hemometer, first place the Sahli test tube into the hemometer standard. Then, carefully pour 0.1 N HCl into the test tube until it reaches the second line on the tube.

How is blood drawn for the test using the Sahli method?

Blood is drawn using a Sahli pipette attached to a hose. Before using the pipette, it should be cleaned with alcohol and dried. Blood is then slowly drawn into the pipette until it reaches the 0.02 mL mark.

What steps are involved in mixing the blood with the hydrochloric acid solution?

Once the blood is drawn into the Sahli pipette, it is transferred into the test tube containing the HCl solution. The mixture is then stirred gently using a stirring rod to ensure proper homogenization.

Why is it necessary to incubate the mixture of blood and HCl?

The mixture of blood and HCl is incubated at room temperature for 3 minutes to allow for proper reaction and color development, which is essential for accurate comparison with the hemometer standard.

How do you adjust the color of the blood-HCl mixture to match the hemometer standard?

After the incubation, you add aquades (distilled water) drop by drop while stirring to adjust the color of the mixture. The goal is to make the color of the solution in the test tube match the color of the hemometer standard.

How do you determine when the color of the solution matches the hemometer standard?

You compare the color of the mixture in the test tube with the hemometer standard by positioning your eyes at the same level as the bottom curve (meniscus) of the liquid. When the color matches, the reading is taken.

What is the normal range for hemoglobin levels in adults, and how does it relate to the Sahli method?

The normal hemoglobin levels are 12-16 grams per deciliter for women and 14-18 grams per deciliter for men. The Sahli method is used to measure the hemoglobin concentration, which can help identify abnormal levels that might indicate conditions like anemia.

What factors could cause abnormal hemoglobin levels, as mentioned in the script?

Abnormal hemoglobin levels can be caused by various factors. Low hemoglobin (anemia) can result from kidney disease, excessive intravenous fluid administration, or certain medications. High hemoglobin levels can occur due to dehydration, chronic obstructive pulmonary disease (COPD), congestive heart failure, or burns.