Hemoglobin Metode Sahli [Hb Sahli] - TUTORIAL LENGKAP DISETIAP TAHAP ! SAMPAI TUNTAS !!

Summary

In this video, the presenter introduces the Sahli method for hemoglobin testing, detailing the necessary equipment and procedures. The process involves mixing a blood sample with hydrochloric acid, achieving a brownish color that indicates hemoglobin levels. After incubation, distilled water is added dropwise until the sample matches a color standard. The hemoglobin concentration is read from a scale, with normal ranges specified for both men and women. The session concludes with students required to fill out a laboratory report and properly clean up their work area, emphasizing the importance of procedural accuracy in hematology practices.

Takeaways

• 😀 The aim of the Sahli method is to determine the hemoglobin concentration in blood.
• 🧪 The Sahli method is a professional technique using a hemometer and standard color solutions.
• 💧 The procedure involves mixing blood with 0.1N HCl until a brown color, indicating acid lewatin, is achieved.
• 🔬 Blood is pipetted after homogenization, ensuring accurate measurement.
• ⏳ After mixing blood and HCl, the sample is incubated for 5 minutes for optimal reaction.
• 🌊 Aquades is added drop by drop until the color matches the standard reference.
• 🔄 Continuous mixing ensures uniformity of the sample during the procedure.
• 📏 The hemoglobin level is read from the scale on the Sahli tube, indicating the concentration in grams per deciliter.
• 📊 Normal hemoglobin levels are between 13.5-17.5 g/dL for men and 11.5-15.5 g/dL for women.
• 📝 After the experiment, students must complete a report and properly clean their equipment.

Q & A

What is the purpose of the Sahli method for hemoglobin testing?

The Sahli method is used to determine the concentration of hemoglobin in the blood.

What materials are needed for the Sahli hemoglobin test?

The test requires a color standard, a pipette, a Sahli tube, a stirring rod, and an aspirator.

What is the principle behind the Sahli hemoglobin test?

The principle involves mixing blood with 0.1 N hydrochloric acid until it changes to a brown color, known as acid hematin.

How is the blood sample prepared before testing?

The blood sample is homogenized before being pipetted into the Sahli tube.

What should be done after adding hydrochloric acid to the blood sample?

After adding hydrochloric acid, the mixture should be incubated for 5 minutes.

How is distilled water used in the Sahli method?

Distilled water is added drop by drop until the color of the solution matches the standard comparison color.

What indicates that the hemoglobin concentration is measured correctly?

The hemoglobin concentration is indicated by the scale on the Sahli tube, which shows the value in grams per deciliter.

What are the normal hemoglobin levels for males and females?

Normal hemoglobin levels for males range from 13.5 to 17.5 grams per deciliter, while for females, it ranges from 11.5 to 15.5 grams per deciliter.

What should students do after completing the practical session?

Students are required to fill out a daily practical report and clean the equipment according to the regulations.

What information must be included in the practical report?

The practical report should include the date, name, student ID, type of test, method used, principles, materials, procedure, results, reference values, conclusions, and discussion.