K3 (Kesehatan dan Keselamatan Kerja) Laboratorium Teknologi Pangan dan Hasil Pertanian
Summary
TLDRThis video script emphasizes laboratory safety and health protocols for research smoothness. It advises reading lab rules, understanding chemical reagent properties and safety data, and using protective gear. It also covers the importance of cleaning work areas, knowing safety equipment locations, handling volatile chemicals in fume hoods, and proper waste disposal. Additionally, it highlights the need for sterilization of equipment and media in autoclaves, careful handling of glassware, and regular equipment checks to prevent accidents, concluding with the reminder to prioritize safety.
Takeaways
- 📚 Read and follow all laboratory safety protocols before starting work.
- 🧪 Always review the Material Safety Data Sheet (MSDS) for each chemical reagent used.
- 👔 Properly wear laboratory coats and ensure they are securely fastened to protect your body and clothing.
- 🧤 Use additional personal protective equipment (PPE) as needed, such as gloves, safety glasses, and masks.
- 👟 Wear comfortable and recommended closed-toe footwear to protect from chemical spills.
- 🧼 Always clean your work area before and after conducting experiments.
- 🚨 Be aware of the location of safety equipment such as fire extinguishers and first aid kits.
- 🌫 Use fume hoods properly when handling volatile chemicals to prevent inhalation.
- 🔥 Keep flammable solvents away from open flames like Bunsen burners.
- 🗑 Properly dispose of research waste according to its classification, such as non-hazardous, hazardous, and glass waste.
- 🔬 Be cautious when using glassware to prevent accidents and dispose of broken glass in designated containers.
- 💡 Ensure that gas cylinders are safely secured with regulators, bodies, and pressure checks before and after use.
- 🔌 Perform visual inspections on electrical equipment before use and avoid using unsafe equipment.
- 📝 Always update the logbook with details of equipment used and their condition after use.
Q & A
What is the primary purpose of the video?
-The primary purpose of the video is to demonstrate the application of K3 (Health, Safety, and Environment) principles in a laboratory setting to ensure personal safety and support smooth research operations.
What is the first thing one should do before starting work in a laboratory?
-Before starting work in a laboratory, one should read and pay attention to every safety regulation present in that laboratory.
Why is it important to read the Material Safety Data Sheet (MSDS) before working with chemical reagents?
-Reading the MSDS is important to understand the hazardous chemical symbols, properties, and incompatible materials of the reagents to ensure safety while handling them.
What should one do before using laboratory equipment?
-One should read the equipment's operating instructions carefully before using any laboratory equipment.
What is the requirement for laboratory coats when working in a lab?
-Laboratory coats must be worn correctly at all times while working in the lab, with all fasteners securely closed to protect the body and clothing effectively.
What additional personal protective equipment might be required in a laboratory?
-Additional personal protective equipment may include gloves to protect from organic solvents, heat-resistant gloves, and masks for those using kerudung (headscarves). Hair should be tied back neatly if not covered by a kerudung.
Why is it necessary to clean the work area before and after working in a laboratory?
-Cleaning the work area before and after working is necessary to prevent accidents and contamination, ensuring a safe and sterile working environment.
What should be considered when disposing of research waste?
-Research waste should be separated according to its type, such as non-hazardous waste, hazardous waste, and glass waste, and disposed of properly.
What precautions should be taken when using flammable solvents near open flames?
-Flammable solvents should be kept away from open flames like a Bunsen burner to prevent fires or explosions.
What is the procedure for using an autoclave?
-Before using an autoclave, ensure there is enough water, close the autoclave tightly, set the temperature and time according to the needs, and turn on the machine. After autoclaving is complete, check that the pressure has returned to normal and the temperature has reached approximately 50 degrees Celsius before handling the sterilized items with heat-resistant gloves.
Why is it important to log the use of equipment in a logbook?
-Logging the use of equipment in a logbook is important for tracking the equipment's usage, maintenance, and condition, which aids in maintaining safety and functionality.
What is the final reminder given in the video regarding safety in the laboratory?
-The final reminder is to always prioritize workplace safety when working in a laboratory.
Outlines
🧪 Lab Safety and Health Measures
This paragraph emphasizes the importance of adhering to safety protocols in the laboratory to ensure personal safety and support smooth research processes. It outlines the necessity of reading and following laboratory rules, understanding the properties of chemical reagents, and wearing appropriate personal protective equipment (PPE) such as lab coats, gloves, and masks. Additionally, it stresses the importance of cleaning the workspace before and after work, knowing the location of safety equipment, and handling volatile chemicals with care. The paragraph also covers the proper use of equipment like autoclaves and the segregation of research waste.
🔬 Safe Laboratory Practices
The second paragraph focuses on specific safety measures to be observed in the lab. It advises against using damaged glassware and disposing of broken pieces properly. It also highlights the importance of checking the safety of gas cylinders and electrical equipment before use. The paragraph stresses the need to keep the workspace tidy and safe to prevent accidents and to always update the equipment log book with personal data and the condition of the equipment used. It concludes with a reminder to prioritize workplace safety in the laboratory.
Mindmap
Keywords
💡K3
💡Laboratory Safety
💡Material Safety Data Sheet (MSDS)
💡Personal Protective Equipment (PPE)
💡Chemical Compatibility
💡Autoclave
💡Laminar Flow
💡Waste Disposal
💡Glassware
💡Electrical Safety
💡Log Book
Highlights
Welcome to the safety and health at work video by the Department of Occupational Safety and Health.
Demonstrates the implementation of K3 in the laboratory to ensure personal safety and support smooth research.
Each laboratory has its own rules that must be followed.
Read and pay attention to each rule before starting work in the laboratory.
Ensure to read the Material Safety Data Sheet (MSDS) before working with chemical reagents.
Pay attention to the hazardous chemical symbols on the reagents.
Consider the properties of the chemical substances, including incompatible chemicals.
Read the equipment instructions carefully before using laboratory tools.
Laboratory coats must be worn properly during work.
Protect your body and clothes by wearing additional personal protective equipment as needed.
Tie back hair neatly and wear comfortable, closed shoes to protect from chemical spills.
Always clean the work area before and after working.
Be aware of the location of safety equipment in the laboratory.
When using volatile reagents, perform evaporation in a fume hood.
Ensure all equipment and body parts entering the laminar flow hood are sterilized with disinfectant.
Keep flammable solvents away from open flames like Bunsen burners.
Dispose of research waste according to its category: non-hazardous, hazardous, and glass waste.
Before starting the autoclave, ensure there is enough water and close the autoclave tightly.
Set the temperature and time according to the needs and turn on the autoclave.
After autoclaving is complete, ensure the pressure has returned to normal and the temperature has reached approximately 50 degrees Celsius.
Use heat-resistant gloves to handle equipment and media that have been autoclaved.
Do not dispose of microbial culture waste directly but autoclave it first at 121 degrees Celsius for 20 minutes.
In the laboratory, pay attention to the use of glassware to prevent work accidents.
Ensure the safety of gas cylinders such as regulators, bodies, and pressure before and after use.
For electrical equipment, perform a visual inspection before use and do not use equipment that is unsafe.
Ensure that tools and materials in the work area are tidy and safe to prevent work accidents.
After using tools, always fill in the logbook with personal data and the last condition of the tools used.
Always prioritize safety when working in the laboratory.
Transcripts
Hai
[Musik]
selamat datang di video keselamatan dan
kesehatan kerja di departemen tphp video
ini menampilkan contoh-contoh penerapan
K3 di laboratorium untuk menjaga
keselamatan diri dan mendukung
kelancaran riset yang akan dilakukan
[Musik]
setiap laboratorium
memiliki tata tertib yang harus diikuti
oleh karena itu sebelum memulai bekerja
bacalah dan perhatikan setiap tata
tertib yang ada di laboratorium tersebut
sebelum bekerja dengan reagen kimia
pastikan untuk membaca material safety
data sheet atau MSDS untuk masing-masing
reagen
perhatikan simbol-simbol
kimia berbahaya yang ada pada reagen
tersebut perhatikan juga sifat-sifat
bahan kimia tersebut termasuk
bahan-bahan kimia lain yang incompatible
[Musik]
bacalah instruksi kerja peralatan dengan
cermat sebelum menggunakan
peralatan-peralatan yang ada di
laboratorium
[Musik]
jas laboratorium wajib dipakai dengan
benar selama bekerja di laboratorium
kancingkan jas secara sempurna jangan
membukanya sebagian agar tubuh dan
pakaian terlindungi dengan baik
pakailah alat pelindung diri lainnya
sesuai kebutuhan seperti memakai sarung
tangan pelindung dari pelarut organik
sarung tangan tahan panas Google ataupun
masker bagi yang menggunakan kerudung
masuk Hai kain kerudung ke dalam jas
laboratorium dengan rapi dan bagi yang
tidak menggunakan kerudung rambut harus
diikat dengan rapi
gunakan alas kaki yang nyaman dan
dianjurkan tertutup untuk melindungi
diri dari tetesan bahan kimia
[Musik]
jangan lupa selalu membersihkan area
kerja baik sebelum dan sesudah bekerja
perhatikan letak peralatan keselamatan
sebelum bekerja di laboratorium seperti
lokasi syur dan wastafel
kotak P3K
dan apa
[Musik]
saat menggunakan reagen yang mudah
menguap penuangan reagen dilakukan dalam
lemari asap Nyalakan blower dan buka
pintu lemari diam saja untuk mencegah
uap terhirup
jika kita bekerja dalam laminar air flow
tol AF pastikan seluruh bagian peralatan
dan tubuh yang masuk di dalam lab telah
disterilkan dengan disinfektan
jauhkan solvent yang mudah terbakar dari
penggunaan api terbuka seperti bunsen
[Musik]
pisahkan limbah sisa penelitian sesuai
tempatnya seperti limbah non B3
limbah B3
dan limbah kaca
sebelum memulai autoklaf pastikan air
dalam autoklaf cukup tambahkan sampai
batas yang disyaratkan Bila perlu
Hai alat dan media yang akan di otoklaf
harus terbungkus atau tertutup rapat
masukkan alat dan media ke dalam otoklaf
dan tutup autoklaf rapat-rapat
atur suhu dan waktu sesuai kebutuhan dan
Nyalakan mesin otoklaf
setelah selesai autoklaf pastikan
tekanan sudah kembali ke normal dan suhu
telah mencapai kurang lebih 50 derajat
Celcius
gunakan sarung tangan tahan panas untuk
mengambil peralatan dan media yang sudah
diautoklaf
[Musik]
limbah kultur mikrobia tidak boleh
langsung dibuang tetapi harus diautoklaf
terlebih dahulu pada suhu 121 derajat
Celcius selama 20 menit
di
dalam bekerja dilaboratorium kita juga
perlu memperhatikan beberapa hal sebagai
berikut seperti penggunaan alat gelas
dengan hati-hati untuk mencegah
kecelakaan kerja jangan menggunakan alat
gelas yang sudah cacat dan buang pecahan
ke wadah limbah kaca
untuk pemakaian tabung gas pastikan
keamanan tabung seperti regulator gunoz
bodi dan tekanan gas sebelum dan sesudah
menggunakannya
untuk peralatan listrik lakukan
pemeriksaan visual sebelum
menggunakannya jangan gunakan peralatan
yang sudah tidak aman
[Musik]
pastikan juga peralatan dan bahan-bahan
di area kerja tertata rapi dan aman
untuk mencegah kecelakaan kerja setiap
setelah menggunakan alat jangan lupa
selalu isi log book peralatan dengan
mengisikan data diri dan kondisi
terakhir alat yang digunakan
[Musik]
Ayo menurutmu
[Musik]
ingat ya selalu Utamakan keselamatan
kerja saat bekerja di laboratorium
semoga bermanfaat
[Musik]
hai hai
[Musik]
Hai dulu ya
[Tepuk tangan]
hai hai
[Musik]
hai hai
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