Pengamatan SEL EPITEL Pipi Manusia

Summary

In this educational video, Ahmad Baihaqi, a biology teacher, walks through a practical exercise on cell observation, focusing on epithelial cells from the human mouth. The process involves two key stages: preparing the cell sample and observing it under a microscope. The preparation includes sterilizing equipment, applying a methylene blue stain, and creating a thin layer on a glass slide. The observation, using an Olympus microscope, starts with low magnification to locate the cells, followed by higher magnifications to clearly observe the cell's structure, including the nucleus and membrane.

Takeaways

• 😀 The script demonstrates the process of cell observation, specifically focusing on epithelial cells from the inner cheek.
• 😀 The session is conducted by Ahmad Baihaqi, a biology teacher, and the students are from class 11 IPA 1.
• 😀 The observation involves two main stages: cell preparation and observation using a microscope.
• 😀 In the first stage, tools such as a microscope, stainless pipette, methylene blue solution, and a beaker are required for the practice.
• 😀 Cell preparation includes sterilizing the tools, using a spatula to collect a small amount of cells, and placing them on an object glass slide.
• 😀 After spreading the cells, a methylene blue solution is applied to the slide to stain the cells for better observation.
• 😀 The second stage involves using a microscope for the observation, starting with an objective magnification of 4x to locate the cells.
• 😀 Once cells are located, the magnification is increased to 10x and later to 40x for more detailed observation.
• 😀 Adjusting the focus using a micrometer is essential for clear observation as different magnifications require different focus settings.
• 😀 The script concludes with the observation of the epithelial cells, highlighting the visibility of the nucleus, cell membrane, and structure, along with a reminder about adjusting the diaphragm for better contrast.

Q & A

What is the main focus of the practical activity described in the video?

The main focus of the practical activity is cell observation, specifically observing epithelial cells using a microscope, with a focus on preparing the slides and adjusting the microscope for clear observation.

Why is methylene blue solution used in the cell preparation?

Methylene blue is used to stain the cells, making the structure of the cells, including the nucleus, more visible under the microscope.

What tools are necessary for the cell observation practice?

The necessary tools for the practice include a microscope, a microscope camera, a flash, a stainless pipette, a glass slide, methylene blue solution, and a beaker.

What is the first step in the cell observation process?

The first step is cell preparation, which involves sterilizing the equipment with alcohol, using a spatula to collect cells, placing them on the glass slide, and staining them with methylene blue.

What role does the alcohol sterilization play in the preparation process?

Alcohol sterilization is used to ensure that all the tools are free of contaminants, preventing any interference with the observation and maintaining hygiene.

How is the focus adjusted during the observation?

The focus is adjusted using a micrometer to ensure that the cells are clearly visible, particularly when switching between objective lenses of different magnifications.

Why is it important to start with a 4x objective lens when observing the cells?

Starting with the 4x objective lens helps to locate the cells easily in the field of view before switching to higher magnifications for a more detailed observation.

What can be seen when using the 40x objective lens to observe the epithelial cells?

At 40x magnification, the structure of the epithelial cells becomes clearer, including the nucleus and cell membrane, as well as the irregular shape and thinness of the cells.

How does adjusting the diaphragm affect the observation?

Adjusting the diaphragm changes the contrast of the image, which allows for better visibility of the contours and structure of the cells.

What is the final outcome of the observation process?

The final outcome is the successful observation of epithelial cells with visible structures, such as the nucleus and membrane, and a clearer understanding of cell morphology.