Materi Fitokimia - Praktikum Ekstraksi Metode Maserasi

Summary

This video outlines a step-by-step guide for extracting compounds from Jati Belanda leaves using a non-heating extraction method. The process involves finely grinding the leaves, mixing with a solvent, and allowing it to rest for 3-5 days, with regular stirring. After multiple rounds of soaking and filtering, the mixture is further processed using a rotary evaporator to separate the extract. The method preserves heat-sensitive compounds, making it ideal for extracting substances that degrade with heat. The technique is suitable for creating potent extracts while protecting the active compounds.

Takeaways

• 😀 Grind the Belanda teak leaves using a blender, then sift with a mesh sieve of 22 mesh size.
• 😀 Weigh the finely ground material, using 100 grams for the extraction process.
• 😀 Place the weighed material into a container and add a solvent to fully moisten it.
• 😀 Mix the material thoroughly, following a ratio of 1 part of the herb to 10 parts of solvent.
• 😀 Seal the container, keeping it in a dark place for 3-5 days and stir the mixture regularly.
• 😀 Ensure the container is shielded from light and air by covering it with black plastic.
• 😀 Continue stirring the mixture every 6 hours during the 3-5 day period.
• 😀 After extraction, filter the mixture using flannel cloth to separate small herb particles.
• 😀 Repeat the soaking process with the same solvent up to three times for thorough extraction.
• 😀 Use filter paper to perform an additional filtration to ensure no fine particles remain.
• 😀 After completing the extraction and filtration, use a rotary evaporator to remove excess solvent.
• 😀 Once the liquid extract is obtained, use a water bath to further evaporate the water content.
• 😀 This method of extraction is referred to as cold extraction, ideal for heat-sensitive compounds.

Q & A

What is the first step in the extraction process described in the script?

The first step is to finely grind the Jati Belanda leaves using a blender and then sift them to a size of 22 mesh.

How much Jati Belanda leaf is needed for the extraction process?

100 grams of the ground and sifted Jati Belanda leaves are needed for the extraction.

What is the correct ratio of leaves to solvent for the extraction?

The correct ratio is 1 part of the leaves to 10 parts of solvent.

How long should the mixture of leaves and solvent be left to steep?

The mixture should be left to steep for 3-5 days in a dark place, with periodic stirring every 6 hours.

Why is it important to cover the container with black plastic?

The black plastic is used to protect the mixture from light, air, and moisture, which could affect the extraction process.

What tool is used to filter the mixture after the steeping process?

A flannel cloth is used to filter the mixture and separate the solid particles from the liquid extract.

How many times should the mixture be soaked with the same solvent?

The mixture should be soaked and filtered up to three times using the same solvent.

What are the key steps involved in the solvent removal process?

The solvent is first removed using a rotary evaporator, followed by further evaporation using a water bath to remove any remaining water content.

What is the purpose of using a rotary evaporator in the process?

The rotary evaporator is used to efficiently remove most of the solvent, leaving behind a concentrated liquid extract.

Why is this extraction method referred to as a cold extraction process?

This method is considered a cold extraction because the samples are not exposed to heat, which is ideal for extracting heat-sensitive compounds.