Praktikum Farmakologi : Uji Analgetik

Summary

This video demonstrates an experiment comparing the analgesic effects of paracetamol and acetosal (aspirin) on mice. The study uses acetic acid to induce pain and observes the animals' response. Three groups are treated: one with paracetamol, another with acetosal, and the last as a control group. Pain response is measured by counting wriggles over an hour. Results show that paracetamol provided higher pain relief than acetosal, aligning with previous studies. The video highlights the importance of proper treatment and methods in achieving accurate experimental results.

Takeaways

• 😀 The experiment is focused on comparing the analgesic effects of Paracetamol and Acetosal (Aspirin) using mice as subjects.
• 😀 The pain induction for the experiment is achieved using a 1% acetic acid solution injected intraperitoneally into the mice.
• 😀 Three groups of mice are involved in the experiment: one treated with Paracetamol, one with Acetosal, and one control group receiving only CMC-Na.
• 😀 The procedure includes suspending the medication in a liquid form and administering it slowly to ensure proper absorption.
• 😀 After the first treatment, a 5-minute pause is observed before the pain induction with acetic acid takes place.
• 😀 The mice are observed for pain reactions, specifically noting wriggling behavior, which is used to quantify pain response.
• 😀 The wriggling behavior is measured every 5 minutes for 60 minutes to track the pain response over time.
• 😀 The data from the number of wriggles is used to calculate the analgesic effectiveness using a formula based on the control group's response.
• 😀 The results suggest that the Paracetamol group had a higher analgesic effect compared to the Acetosal group.
• 😀 The experiment concludes with a discussion about the various factors that can influence the results, such as treatment methods and external conditions, which could affect the outcome.

Q & A

What is the primary focus of the experiment described in the video?

The primary focus of the experiment is to compare the analgesic effects of paracetamol and acetosal (aspirin) using pain induction in mice.

How is pain induced in the test animals during the experiment?

Pain is induced using 1% acetic acid, which is injected intraperitoneally into the mice.

What groups of mice were used in the experiment?

There are three groups of mice: Group 1, treated with paracetamol; Group 2, treated with acetosal (aspirin); and Group 3, the control group, which does not receive treatment.

What materials are used in the experiment for administering the treatments?

The treatments are administered using oral squid with an ideal size of 27 alcohol cotton, along with a suspension of either paracetamol or acetosal, and 1% acetic acid for pain induction.

How is the test substance administered to the mice?

The test substances (paracetamol and acetosal) are given orally as a suspension to the mice.

What is the role of the control group in this experiment?

The control group is used as a baseline to compare the effects of the analgesic treatments. This group only receives a suspension of CMC-Na (carboxymethyl cellulose) and no pain treatment.

What observations are made after administering acetic acid to the mice?

The mice are observed for signs of discomfort, such as stretching or pulling their bodies forward, which indicates the induction of pain due to the acetic acid.

What is the duration of the observation period after the pain induction?

The mice are observed for 60 minutes, with measurements taken every five minutes during this time.

How is the analgesic effect measured in the experiment?

The analgesic effect is measured by counting the number of wriggles or movements made by the test animals and calculating the percentage of pain relief using the formula: 100 - (E-perkap × 100), where E is the number of wriggles in the treatment group and perkap is the number of wriggles in the control group.

What were the results regarding the analgesic effects of paracetamol and acetosal?

The results showed that acetosal (aspirin) provided a higher analgesic effect than paracetamol, which aligns with findings in related journals and literature.