Tutorial Pemeriksaan HDL Metode Direct Dengan Fotometer

Summary

In this video, the presenter from Universitas Negeri Makassar explains how to conduct an HDL (High-Density Lipoprotein) cholesterol test, a key procedure to measure HDL levels and prevent cardiovascular diseases. The process involves using various materials such as HDL kits, reagents, and micro-pipettes, along with a centrifuge and spectrophotometer to measure the sample's absorbance. The test aims to determine whether HDL levels are normal (above 45 mg/dL), crucial for removing harmful cholesterol from the body and reducing the risk of heart diseases like strokes. The presentation highlights the importance of HDL for heart health.

Takeaways

• 😀 The speaker introduces themselves as Ellison, a student from the Medical Laboratory Technology program at Universitas Negeri Makassar.
• 😀 The purpose of the examination is to measure HDL (high-density lipoprotein) levels in the body to prevent the formation of plaques or atherosclerosis that may lead to heart disease.
• 😀 The tools and materials used for the test include HDL cholesterol kit, reagents, micropipettes, test tubes, and a stopwatch.
• 😀 The first step of the procedure involves creating a precipitate by mixing HDL cholesterol and serum samples in a reaction tube and homogenizing the mixture.
• 😀 The sample is then centrifuged at 4000 RPM for 10 minutes to separate the precipitate and supernatant, with the supernatant being used for further testing.
• 😀 Cholesterol reagent is added to the tubes to optimize the enzymatic reaction, which will cause a color change that can be measured by a photometer.
• 😀 The photometer reads the absorbance of the blank, standard, and test samples, providing numerical values for HDL concentration.
• 😀 The standard reagent used is a known concentration of cholesterol to compare the test sample against.
• 😀 After incubation, the test sample is measured on the photometer, and the absorbance values are used to calculate the HDL level.
• 😀 The desired HDL value is above 45 mg/dL for normal health, and lower levels may lead to conditions like heart disease or stroke.
• 😀 The speaker concludes by emphasizing the importance of HDL in carrying harmful cholesterol from the body and preventing cardiovascular issues.

Q & A

What is the primary purpose of the HDL cholesterol test?

The primary purpose of the HDL cholesterol test is to measure the level of HDL (High-Density Lipoprotein) in the body. This helps prevent the formation of plaques and atherosclerosis, which could lead to heart disease.

What does HDL cholesterol do in the body?

HDL cholesterol helps carry harmful cholesterol away from the arteries to the liver, where it can be processed and eliminated from the body. This helps protect against heart disease and stroke.

What are the main tools and materials required for conducting the HDL test?

The main tools and materials required for the HDL test include an HDL test kit, micro pipettes (100-1000 microns and 10-100 microns), a test tube, cholesterol reagent, a spectrophotometer, a stopwatch, and tissue paper.

What is the first step in the HDL test procedure?

The first step is to prepare the sample by pipetting 500 microliters of HDL cholesterol into a test tube, followed by adding 500 microliters of the serum sample. The contents are then mixed thoroughly.

Why is centrifugation performed during the HDL test?

Centrifugation is performed to separate the precipitate (solid particles) from the supernatant (liquid), which is needed for the subsequent steps of the test. This ensures the accuracy of the test results.

What is the role of the cholesterol reagent in the HDL test?

The cholesterol reagent is used to optimize the enzymatic reaction in the test, leading to a color change, which can then be measured by the spectrophotometer to determine the HDL level.

How is the HDL test result measured?

The test result is measured by reading the absorbance values of the test, standard, and blank samples using a photometer. The device automatically calculates the HDL concentration based on these absorbance values.

What should the HDL level be for it to be considered normal?

A normal HDL level should be above 45 mg/dL. HDL levels lower than this may indicate an increased risk of heart disease and stroke.

What happens if the HDL level is low?

Low HDL levels can lead to the accumulation of harmful cholesterol in the arteries, which can increase the risk of heart disease, stroke, and other cardiovascular issues.

How long and at what temperature are the test samples incubated?

The test samples are incubated at 30°C for 5 minutes to allow the enzymatic reactions to occur and to achieve accurate measurement of the HDL concentration.