Praktikum Pembuatan Medium

Summary

This transcript describes a laboratory simulation for preparing and sterilizing both synthetic and non-synthetic media. The process covers making Potato Dextrose Agar (PDA) and Tofu Extract Agar (TEA). It details the steps from preparing the main ingredients—potatoes, dextrose, agar, and water—for PDA, to sterilizing the medium in an autoclave. Similar steps are followed for the TEA medium, involving tofu extract. The video also touches on sterilization techniques, ensuring accurate measurements, and maintaining the appropriate conditions for successful medium preparation in laboratory settings.

Takeaways

• 😀 The experiment involves two main tasks: preparation of synthetic media and sterilization.
• 😀 For non-synthetic media, Potato Dextrose Agar (PDA) and Toge Extract Agar (TEA) will be prepared.
• 😀 Potato Dextrose Agar (PDA) requires 250g of potatoes, 5g of dextrose, and 3g of agar in 255 ml of water.
• 😀 The process begins with boiling the potatoes in water for 15-20 minutes before straining and mixing in additional ingredients.
• 😀 Once ingredients are added to an Erlenmeyer flask, it is topped off with water and homogenized.
• 😀 The flask is then covered with cotton and sealed with paper, followed by sterilization in an autoclave at 121°C for 15 minutes.
• 😀 For Toge Extract Agar (TEA), 100g of soybeans, 2g of dextrose, and 1.2g of agar are used to create 100 ml of medium.
• 😀 Soybeans are boiled until wilted, then combined with dextrose and agar in a similar process as PDA preparation.
• 😀 After homogenizing, the TEA mixture is sealed and sterilized in an autoclave at 121°C for 15 minutes.
• 😀 The script also describes a compound medium, which requires 5g of medium powder dissolved in 250 ml of water, followed by sterilization at 121°C for 15 minutes.

Q & A

What is the purpose of this laboratory simulation?

The simulation aims to demonstrate the process of creating synthetic and non-synthetic media, as well as sterilizing them, specifically by making Potato Dextrose Agar (PDA) and Toge Extract Agar (TEA).

What are the main ingredients needed to prepare Potato Dextrose Agar (PDA)?

To prepare Potato Dextrose Agar (PDA), the main ingredients are potatoes, dextrose (5g), agar (3g), and water.

How long should the potatoes be heated to prepare PDA?

The potatoes should be heated for 15 to 20 minutes to ensure they are properly softened for medium preparation.

How much potato is required to make 255 mL of Potato Dextrose Agar?

To make 255 mL of Potato Dextrose Agar, 250 grams of potatoes are needed.

What is the procedure for adding dextrose and agar to the Erlenmeyer flask when preparing PDA?

After boiling the potatoes, dextrose (5g) and agar (3g) are added to the Erlenmeyer flask. The solution is then topped off to the correct volume (255 mL) to maintain accuracy.

Why is it important to ensure that the Erlenmeyer flask is filled up to the correct level?

It is important to fill the Erlenmeyer flask up to the correct level to ensure accurate measurements and prevent errors in the composition of the medium.

How is the PDA medium sterilized after preparation?

The PDA medium is sterilized by placing it in an autoclave at 121°C for 15 minutes.

What ingredients are needed to make Toge Extract Agar (TEA)?

To make Toge Extract Agar (TEA), the ingredients required are 100 grams of soybean sprouts (toge), 2 grams of dextrose, 1.2 grams of agar, and water.

What is the process for preparing the Toge Extract Agar medium?

To prepare Toge Extract Agar, the soybean sprouts are boiled until soft. Then, the sprouts are placed in an Erlenmeyer flask along with dextrose and agar. The flask is filled up to the required volume, homogenized, and sterilized in an autoclave at 121°C for 15 minutes.

Why should the Erlenmeyer flask be covered with paper and rubber bands during sterilization?

The Erlenmeyer flask is covered with paper and rubber bands to prevent contamination and ensure the sterilization process is effective, while also protecting the contents from external elements.