Cara Membuat Preparat Basah untuk Pengamatan Mikroskop

Summary

This tutorial teaches how to make wet preparations for microscopic observation. It covers essential tools and methods, such as slide glass, cover glass, and pipettes, to prepare both plant and animal cell specimens. The process includes preparing onion epidermis, plant cells, and human cheek cells, offering clear, step-by-step instructions. Emphasis is placed on using liquid media for wet preparations, handling materials carefully, and cleaning up afterward. The video provides valuable insights for beginners looking to understand the practical aspects of sample preparation and microscopic observation.

Takeaways

• 😀 Wet preparations are one of the three types of microscope specimen preparations, along with dry and preserved preparations.
• 😀 Wet preparations are used to observe specimens in a liquid state, such as plant and animal cells or liquid samples like water and body fluids.
• 😀 Basic tools required for wet preparations include slide glass, cover glass, a dropper pipette, and a small beaker for liquid samples.
• 😀 When using a dropper pipette, it is important to press the rubber, dip it into the liquid, release the rubber to draw up the liquid, and then drop it carefully onto the slide.
• 😀 After adding the liquid to the slide, cover it slowly with a cover glass to avoid trapping air bubbles.
• 😀 Wet preparations can be observed directly under the microscope or stained with dye to enhance visibility.
• 😀 A simple example of plant cell observation is using red onion epidermis, where thin slices are placed on the slide with water before covering with a cover glass.
• 😀 For animal cell observation, a more complex process may be required, including cutting animal organs or using liquid samples like saliva or blood.
• 😀 Observing human cheek cells involves using a toothpick to collect residue from the inner cheek, placing it on the slide, adding water, and covering it with a cover glass.
• 😀 After use, all materials and tools should be cleaned properly to prevent contamination or damage. Slide glasses should be cleaned with soap, and cover glasses with alcohol.
• 😀 Wet preparations are temporary, and any excess water should be absorbed using blotting paper or tissue to prevent damage to the specimen.

Q & A

What are the three main types of preparations for observing under a microscope?

The three main types of preparations are dry preparations, wet preparations, and preserved preparations.

What is the difference between dry preparations and wet preparations?

Dry preparations are observed in a dry state and are typically used for dry organic and inorganic objects, while wet preparations are observed in a wet state and are used for specimens like plant cells, animal cells, or liquid samples such as water or body fluids.

What are preserved preparations, and how do they differ from dry and wet preparations?

Preserved preparations are the most complex, involving complicated preservation methods to ensure that specimens remain durable and can be observed multiple times. This differs from dry and wet preparations, which are temporary or simpler.

What tools are necessary for preparing a wet preparation?

For preparing a wet preparation, you need slide glass, cover glass, a dropper pipette, and a small beaker. It’s also useful to have a needle or toothpick to lower the cover glass carefully.

How should you use a dropper pipette when preparing a wet preparation?

To use a dropper pipette, press the rubber bulb, dip the tip into the liquid, release the bulb to draw the liquid into the pipette, and then carefully drop one drop of liquid onto the slide glass.

What should be done if the preparation is too wet after adding the liquid?

If the preparation is too wet, you can use absorbent materials such as blotting paper or tissue to absorb the excess water.

How do you prepare a red onion sample for observation under a microscope?

To prepare a red onion sample, split the onion, then cut a thin slice of the onion epidermis. Place it on the slide, drop water using a dropper, and carefully cover it with a cover glass.

What is the procedure for preparing animal cell samples for observation?

To prepare animal cell samples, you can use a tissue sample from an organ like the intestines, muscles, or kidneys. The animal should be anesthetized or ethically killed. Alternatively, you can use liquid samples such as saliva, blood, or urine, following the same procedure as wet preparations.

How can you prepare human cheek cells for observation under a microscope?

To prepare human cheek cells, use a toothpick to gently scrape the inside of the cheek, then apply the residue onto the slide. Add a drop of water, cover it with a cover glass, and absorb excess water if needed.

Why is it important to clean the tools after using them for wet preparations?

It is important to clean the tools after use because wet preparations are temporary, and cleaning ensures that the tools are ready for the next use and prevents contamination between different samples.