Pemeriksaan Analisis Sperma - Rahmat Azhari Kemal, M.Si

Summary

In this educational video, Rahmat Azhari Kemal, a lecturer from the Faculty of Medicine at the University of Riau, demonstrates a semen analysis practical. The video covers both macroscopic and microscopic evaluations of semen, including color, odor, pH, volume, liquefaction, and viscosity. It also highlights sperm motility and concentration analysis using various lab equipment like microscopes and pipettes. The video provides step-by-step instructions on how to assess sperm quality, including methods for counting motility categories and calculating sperm concentration, aimed at enhancing understanding of semen analysis in a medical context.

Takeaways

• 😀 The video introduces a practical session on semen analysis, conducted by Rahmat Azhari Kemal from the Faculty of Medicine, Universitas Riau.
• 😀 The session covers both macroscopic and microscopic semen analysis procedures.
• 😀 Key tools used in the analysis include a microscope, cell counter, pipettes, glass slides, and sperm samples.
• 😀 Macroscopic observations involve checking semen color, odor, volume, pH, liquefaction, and viscosity.
• 😀 Normal semen color should be pearly white; deviations in color (clear, red, or cloudy) may indicate specific issues such as low sperm count or infections.
• 😀 Semen has a characteristic smell, and unusual odors (like sour or foul) can suggest infection or excessive time after collection.
• 😀 The semen volume is measured using a graduated cylinder, with the volume noted for accuracy in analysis.
• 😀 pH is tested with a universal pH strip, and the typical pH range of semen is between 7 and 8.
• 😀 The liquefaction and viscosity are assessed to ensure proper semen consistency after collection.
• 😀 Microscopic analysis focuses on sperm motility and concentration. Motility categories include progressive, slow-moving, non-progressive, and immotile sperm.
• 😀 To measure sperm concentration, the sample is diluted and analyzed under the microscope. Special methods like using a counting chamber help assess sperm count accurately.

Q & A

What is the purpose of the semen analysis in this practical?

The purpose of the semen analysis in this practical is to evaluate both the macroscopic and microscopic characteristics of the semen sample, such as color, volume, pH, motility, and concentration of spermatozoa.

What equipment is required for this semen analysis practical?

The equipment required includes a microscope, glass slides, cover glass, micropipette, graduated cylinder, microtube, Newbauer chamber, and a computer with connected software for sperm motility and concentration analysis.

How is the color of semen analyzed in the macroscopic examination?

The color of semen is analyzed by observing it on a white paper. Healthy semen should appear pearl white. Variations in color can indicate potential issues: clear semen suggests a low sperm count, red semen indicates the presence of blood, and cloudy semen could indicate leukocytes or infection.

What does the smell of semen indicate during the macroscopic examination?

The smell of semen can suggest different conditions: a characteristic smell from spermine oxidation is normal, while a fishy or sharp odor might indicate an infection, and a foul odor suggests the semen was collected more than six hours prior.

What is the procedure for measuring the volume of semen?

To measure the volume of semen, the sample is transferred from the collection container to a graduated cylinder using a pipette. The volume is then recorded, for example, 1.6 cc in the script.

How is the pH of semen measured?

The pH of semen is measured using a universal pH indicator paper. A strip is dipped into the semen sample, and the pH level is observed, typically ranging between 7-8.

What are the criteria for evaluating semen liquefaction and viscosity?

Semen liquefaction is considered complete when the sample is fully liquid without any clumps or coagulation. Viscosity is evaluated by observing the rate at which semen drops from the end of a pipette.

What steps are involved in the microscopic examination of sperm motility?

For sperm motility analysis, the semen sample is stirred to homogenize it, then a drop is placed on a glass slide and covered with a cover glass. The slide is observed under a microscope to assess four categories of motility: progressive linear, slow or circular, localized, and immotile.

How is sperm concentration measured in this practical?

Sperm concentration is measured by diluting the semen sample with a George solution (1:20 dilution), mixing it, and then placing a drop on a counting chamber. The sperm count is observed under the microscope after allowing the sample to settle.

What is the method used for calculating sperm concentration using the Newbauer chamber?

In the Newbauer chamber, sperm concentration is calculated by counting the sperm cells within a specific large square on the grid. If there are 10-40 spermatozoa in the square, the counting is done in 25 large squares; if there are more than 40, 5 large squares are counted.