Praktikum Teknologi Sediaan Farmasi Liquid dan Semisolid: Pembuatan Sediaan Emulsi Minyak Ikan

Summary

In this video, Fitria Nugrahainy demonstrates the preparation of pharmaceutical liquid and semi-solid formulations. She explains the process of creating liquid suspensions, emulsions, and semi-solid creams, highlighting their respective evaluations. The video also covers the proper lab attire and safety protocols, including the use of lab coats, gloves, and face masks. The key focus is on making an emulsion with fish oil and evaluating it through microscopy to determine whether it’s an oil-in-water or water-in-oil emulsion. The video aims to provide practical knowledge on pharmaceutical preparation techniques and their quality evaluations.

Takeaways

• 😀 Always wear a lab coat, mask, hand gloves, and closed-toe shoes when entering the laboratory.
• 😀 The preparation of liquid suspensions and emulsions involves different methods, with specific equipment and steps for each.
• 😀 For making an emulsion, add water to a beaker, then add the emulsifying agent and mix at 3000rpm for 5 minutes.
• 😀 After preparing the emulsifying agent, add fish oil and mix again at 3000rpm for 10 minutes to form the emulsion.
• 😀 The evaluation of emulsions includes determining the type of emulsion by using a microscope and methylene blue solution.
• 😀 To determine the emulsion type, place a drop of the emulsion on a glass slide, add methylene blue, and observe under the microscope.
• 😀 Emulsions can be classified as either oil-in-water or water-in-oil based on how they interact with methylene blue.
• 😀 Always ensure that the equipment is properly cleaned and sterilized before starting any preparation.
• 😀 During the evaluation of suspensions and emulsions, specific criteria like appearance, stability, and particle size are important.
• 😀 This tutorial provides practical demonstrations of how to prepare pharmaceutical emulsions and suspensions, ensuring proper formulation and quality control.

Q & A

What is the focus of the laboratory session discussed in the video?

The session focuses on the preparation and evaluation of liquid suspensions, emulsions, and semi-solid creams in pharmaceutical practice.

What is the first safety step when entering the laboratory?

The first safety step is to wear a lab coat properly, followed by wearing a mask, gloves, a headset, and closed-toe footwear.

What equipment is needed for preparing an emulsion?

To prepare an emulsion, you will need a beaker, emulsifying agent, water, fish oil, a homogenizer, and a microscope for evaluation.

How should the emulsifying agent be incorporated into the preparation of an emulsion?

The emulsifying agent should be added into the water in the beaker and then mixed using an ultrasonic homogenizer at 3000 rpm for 5 minutes.

What is the next step after mixing the emulsifying agent with water in the preparation of an emulsion?

After mixing the emulsifying agent with water, the next step is to add fish oil and continue mixing with the homogenizer at 3000 rpm for 10 minutes.

What is the purpose of the methylene blue test in evaluating an emulsion?

The methylene blue test is used to determine the type of emulsion (oil-in-water or water-in-oil) by observing the interaction between the emulsion and the dye under a microscope.

What is the difference between evaluating a suspension and an emulsion?

While both suspensions and emulsions require evaluation, the main difference is that emulsions require the determination of the emulsion type, which is done using the methylene blue test, while suspensions focus more on their physical stability and properties.

What is the significance of using a microscope in the emulsion evaluation process?

The microscope is used to visually confirm whether the emulsion is oil-in-water or water-in-oil, by examining the behavior of the methylene blue dye.

What additional safety precautions should be taken when working in the laboratory?

In addition to wearing appropriate safety gear, hands should be sanitized using hand gloves, and proper laboratory techniques should be followed to ensure safety during preparation and evaluation.

How long should the ultrasonic homogenizer be operated when preparing the emulsion?

The ultrasonic homogenizer should be operated at 3000 rpm for 5 minutes when mixing the emulsifying agent with water and for 10 minutes when incorporating the fish oil into the mixture.